Product Details

Uniprot No.

Target Names

CLIC4

Alternative Names

Chloride intracellular channel 4 antibody; Chloride intracellular channel 4 (mitochondrial) antibody; Chloride intracellular channel 4 like antibody; Chloride intracellular channel protein 4 antibody; Clic4 antibody; CLIC4_HUMAN antibody; CLIC4L antibody; DKFZP566G223 antibody; FLJ38640 antibody; H1 antibody; HUH1 antibody; Intracellular chloride ion channel protein p64H1 antibody; MC3S5 antibody; mtCLIC antibody; p64H1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CLIC4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16D7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CLIC4, or chloride intracellular channel protein 4, plays a multifaceted role in cellular physiology, participating in chloride ion transport, apoptosis regulation, and cellular differentiation. This protein has garnered significant research attention due to its involvement in TGF-β signaling pathways and its altered expression patterns in various disease states, including cancer progression and cardiovascular conditions. Understanding CLIC4 function provides valuable insights into membrane dynamics and intracellular signaling mechanisms.

This recombinant monoclonal antibody, clone 16D7, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically to the last, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host origin combined with monoclonal specificity delivers high-affinity binding to human CLIC4, with the antibody raised against a synthetic peptide derived from the human protein sequence.

Validation across multiple experimental platforms demonstrates this antibody's versatility in your research workflow. Western blot analysis confirms specific detection at the expected 29 kDa molecular weight across diverse human cell lines, including HeLa, HEK293, HEK293T, COLO-205, SW620, and HepG2, with recommended dilutions ranging from 1:500 to 1:2000. Immunofluorescence studies in HeLa cells reveal clear intracellular localization patterns, while flow cytometry analysis in U251 glioblastoma cells shows distinct positive population shifts compared to isotype controls.

This antibody serves researchers investigating chloride channel biology, epithelial-mesenchymal transition, tumor microenvironment dynamics, and mitochondrial function, providing a dependable tool for exploring CLIC4's diverse cellular roles.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).

Gene References into Functions

Results provide evidence that the G4 structure formed in the CLIC4 promoter region may act as a regulatory element in regulating CLIC4 gene transcription. PMID: 30201851
CLIC4 and Ihh could serve as biological markers for the progression, metastasis and/or invasiveness of pancreatic ductal adenocarcinoma. PMID: 28205343
in malignant pleural mesothelioma, the gene expressions of CLIC3 and CLIC4 were significantly increased compared to controls PMID: 26445368
CLIC1 and CLIC4 are overexpressed in specific tumor types or their corresponding stroma and change localization and function from hydrophilic cytosolic to integral transmembrane proteins. (Review) PMID: 25546839
CLIC4 knockdown decreases cell-matrix adhesion, cell spreading and integrin signaling, whereas it increases cell motility. PMID: 25344254
This study investigated the proteome modulated by oncogenic KRAS in immortalized airway epithelial cells. PMID: 24503901
Increased CLIC4 expression is an early manifestation and mediator of endothelial dysfunction in pulmonary hypertension. PMID: 24503951
CLIC4 increases tumor cell migration and invasion in a TGF-beta-dependent manner. PMID: 23416981
In addition to CLIC1 and TPM1, which were the proteins initially discovered in a xenograft mouse model, CLIC4, TPM2, TPM3, and TPM4 were present in ovarian cancer patient sera at significantly elevated levels compared with controls. PMID: 23792823
Our data indicate that CLIC4 protein may be a key element in the apoptotic response to oxidative stress. PMID: 23380911
These results demonstrate that CLIC4 nuclear translocation is an integral part of the cellular response to starvation. PMID: 22761775
Reduced CLIC4 expression and nuclear residence detected in cancer cells is associated with the altered redox state of tumor cells and the absence of detectable nuclear CLIC4 in cancers contributes to TGF-beta resistance and enhances tumor development. PMID: 22387366
CLIC4 may not be responsible for benign familial infantile seizures (BFIS) in a Chinese family affected with BFIS. PMID: 20374090
Data suggest that CLIC4 is regulated by RhoA to be targeted to the plasma membrane, where it may function not as an inducible chloride channel but rather by displaying Cys-dependent transferase activity toward a yet unknown substrate. PMID: 19776349
CLIC4 functions to promote endothelial cell proliferation and to regulate endothelial morphogenesis, and is thus involved in multiple steps of in vitro angiogenesis. PMID: 19247789
CLIC4 is differentially regulated in fibroblasts and its expression contributes to a myofibroblast phenotype PMID: 12163372
CLIC4 has alternate cellular functions that are distinct from their proposed roles as chloride channels PMID: 14569596
subcellular localization of CLIC4 in endothelial cells was dependent on whether cells were engaged in proliferation or tube formation PMID: 16239224
up-regulation of mitochondrial CLIC4, together with a reduction in Bcl-2 and Bcl-xL, sensitizes Myc-expressing cells to the proapoptotic action of Bax. PMID: 16316993
preliminary crystallographic analysis PMID: 16842122
CLIC4 in tumor stroma has a role in myofibroblast conversion in human neoplasms PMID: 17200346
Nuclear CLIC4, possibly by altering the Cl(-) and pH of the nucleus, contributes to cell cycle arrest and the specific gene expression program associated with keratinocyte terminal differentiation. PMID: 17636002
Data showed that CLIC1 and CLIC5, but not CLIC4, were strongly and reversibly inhibited (or inactivated) by F-actin. PMID: 18028448
ROS-initiated CLIC4 up-regulation is required for TGF-beta1-induced fibroblast-to-myofibroblast transdifferentiaton in ovarian cancer. PMID: 19639201
S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho-extracellular signal-regulated kinase and chloride intracellular channel 4. PMID: 19713532

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane; Single-pass membrane protein. Nucleus matrix. Cell membrane; Single-pass membrane protein. Mitochondrion. Cell junction. Note=Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.

Protein Families

Chloride channel CLIC family

Tissue Specificity

Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.

Database Links

HGNC: 13518

OMIM: 606536

KEGG: hsa:25932

STRING: 9606.ENSP00000363500

UniGene: Hs.440544

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Code	CSB-RA056014A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(20µg),HEK293 whole cell lysate(20µg), HEK293T whole cell lysate(20µg), COLO-205 whole cell lysate(20µg),SW620 whole cell lysate(20µg),HepG2 whole cell lysate(20µg) All lanes: CLIC4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time：3min Immunofluorescence staining of Hela cell with CSB-RA056014A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing U251 cells stained with CSB-RA056014A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CLIC4 Recombinant Monoclonal Antibody

Product Details

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Target Background