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CRABP2 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA566916A0HU
Size US$210
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  • Western Blot
    Positive WB detected in: HT-29 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg)
    All lanes: CRABP2 polymerase beta antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa
    Exposure time：2min
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Product Details

Uniprot No.
P29373
Target Names
CRABP2
Alternative Names
Cellular retinoic acid-binding protein 2 (Cellular retinoic acid-binding protein II) (CRABP-II), CRABP2
Species Reactivity
Human
Immunogen
A synthesized peptide from human CRABP2 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
12C12
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Cellular retinoic acid-binding protein 2 (CRABP2) serves as a critical intracellular carrier that shuttles retinoic acid from the cytoplasm to nuclear retinoic acid receptors, directly influencing gene transcription programs involved in cell differentiation, proliferation, and apoptosis. This regulatory role makes CRABP2 a protein of significant interest in developmental biology, cancer research, and studies of retinoid signaling pathways, where its expression levels often correlate with cellular differentiation states and therapeutic responses to retinoid-based treatments.

This recombinant monoclonal antibody, clone 12C12, offers the reproducibility and consistency that demanding research applications require. Because it is produced from a defined sequence in rabbit host cells, you can expect uniform performance across experiments and between lot numbers, eliminating the variability that can complicate long-term studies or multi-site collaborations. The antibody is raised against a synthetic peptide derived from human CRABP2 and has been validated for human sample detection.

Western blot validation demonstrates reliable detection of CRABP2 in human cell lysates, with clear bands observed at the expected 16 kDa molecular weight in both HT-29 colorectal adenocarcinoma and SH-SY5Y neuroblastoma cell lines. These results, obtained at 1:1000 dilution with standard exposure times, confirm robust performance across different cellular contexts. The antibody is also validated for ELISA applications, with recommended western blot dilutions ranging from 1:500 to 1:2000, allowing flexibility to optimize signal intensity for your specific experimental conditions.

Whether investigating retinoid signaling mechanisms, exploring CRABP2 as a potential biomarker, or characterizing differentiation pathways in cancer models, this antibody provides a dependable tool for your research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
Gene References into Functions
  1. Semiquantitative and quantitative analyses of the markers RARA and CRABP2 indicate their potential as biomarkers for tumor progression and their participation in nephroblastoma tumorigenesis PMID: 29378601
  2. These data suggest a retinoic acid-independent, non-tumor suppressor role of CRABP2 for the survival of Malignant peripheral nerve sheath tumor (MPNST) cells in vitro. Targeting CRABP2 overexpression may represent a unique approach for the treatment of human MPNST. PMID: 28502478
  3. A high expression ratio between FABP5 and CRABPII may be related to CP tumor recurrence and ATRA could be a potential therapeutic agent for CP chemotherapy. PMID: 27418530
  4. reducing CRABP2 levels may enhance the therapeutic index of Retinoic acid in glioblastoma multiforme patients PMID: 26893190
  5. in esophageal squamous carcinogenesis by significantly inhibiting cell growth, inducing cell apoptosis and blocking cell metastasis both in vitro and in vivo PMID: 26839961
  6. The anticarcinogenic activities of CRABP2 are mediated by both HuR and RAR. PMID: 25320093
  7. CBX3 and CRABP2 expression was markedly increased in lung cancer tissues PMID: 24751108
  8. Reengineering of cellular retinoic acid binding protein II (CRABPII) to be capable of binding retinal as a protonated Schiff base is described PMID: 24059243
  9. CRABP2 controls mRNA stabilization by HuR. PMID: 24687854
  10. overexpression of CRABP-II is a late event of pancreatic carcinogenesis, and it could be used as a diagnostic marker PMID: 24709110
  11. all three proteins (RDH10, RALDH2, and CRABP2) appeared to be required for ATRA production induced by activation of PPARgamma PMID: 23833249
  12. E-FABP showed high exp ression in NSCLC, and the increased E-FABP expression may involved in the occurrence and development of NSCLC PMID: 23327868
  13. Hypermethylation in the CpG island of the CRABP2 gene is associated with astrocytic gliomas. PMID: 22275178
  14. Aberrant methylation in CRABP-II reduces the expression of CRABP-II that in turn confers retinoic acid resistance in medulloblastoma cells. PMID: 22153617
  15. Low CRABP2 is associated with pancreatic ductal adenocarcinoma. PMID: 22010213
  16. Nuclear translocation of cellular retinoic acid-binding protein II is regulated by retinoic acid-controlled SUMOylation PMID: 21998312
  17. Trophoblast spheroids cocultured with endometrial cells overexpressing CRABP2 are defective in expansion and exhibit apoptosis, suggesting that CRABP2 is involved in abnormal endometrium-trophoblast interaction, leading to implantation failure. PMID: 21605859
  18. Data show that NIPER-4 induces cIAP1-mediated ubiquitylation of CRABP-II, resulting in the proteasomal degradation. PMID: 21414315
  19. Individual variation in ALDH1A2/CRABP2 genes may account for subtle variations in retinoic acid-dependent human embryogenesis. PMID: 20308937
  20. Data sghow a higher frequency of CRABP2 and MX1 hypermethylation in primary HNSCC when compared with lymphocytes from healthy individuals. PMID: 20019841
  21. Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest. PMID: 11909957
  22. Cyclin D3 is a cofactor of retinoic acid receptors, modulating their activity in the presence of this protein PMID: 12482873
  23. In this study, we excluded mutations within the complete coding region and the promoter of LMNA and the CRABP II gene in HIV-1 infected patients with lipodystrophy PMID: 12844477
  24. CRABP-II has a role in wound re-epithelialisation by dermal fibroblasts PMID: 14766225
  25. Phosphorylation/dephosphorylation of retinoic x receptor alpha via protein kinase C may be involved in the regulation of CRABP-II gene expression. PMID: 15225641
  26. Data suggest that the ligand-controlled nuclear localization signal of CRABP-II may represent a general mechanism for posttranslational regulation of the subcellular distribution of a protein. PMID: 15866176
  27. Transient transfection of either all-trans-retinoic acid (ATRA) receptor alpha or estrogen receptor alpha expression vectors increased CRABPII expression in MDA-MB-231 cells PMID: 15870697
  28. CRABP-II overexpression in CAKI-2 RCC cells did not significantly influence RA associated antiproliferative actions. PMID: 16215318
  29. MycN binds to the promoter of CRABP-II and induces CRABP-II transcription directly in neuroblastoma. PMID: 16912187
  30. Results describe the structure of apo-wild type (WT) CRABPII at 1.35 A and show that apo- and holo-CRABPII share very similar structures. PMID: 16979656
  31. AP2 factors regulate CRABPII expression in human mammary epithelial cells and breast cancer cells PMID: 17187826
  32. results show that the CRABP2 gene is associated with increased plasma LDL-C concentrations. Carriers of the C allele had significantly higher LDL-C in healthy males, HIV-infected patients, and patients with familial hypercholesterolemia PMID: 17484622
  33. Retinoic acid and retinol regulate expression of cellular retinol binding protein 1 and cellular RA binding protein 2 in fibroid and myometrial cells. PMID: 18343808
  34. MycN and DNA methylation are responsible for CRABP-II expression in pediatric tumors and demethylation of CRABP-II may be an early event in tumor development. PMID: 18955045
  35. Derangement/lack of a critical factor necessary for RARalpha function induces epigenetic repression of a RA-regulated gene network downstream of RARalpha, with major pleiotropic biological outcomes. PMID: 19173001

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Subcellular Location
Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
Protein Families
Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

HGNC: 2339

OMIM: 180231

KEGG: hsa:1382

STRING: 9606.ENSP00000357204

UniGene: Hs.405662

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