CRMP1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA970319A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate(20µg),Mouse brain tissue lysate(20µg) All lanes: CRMP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time：2min IHC image of CSB-RA970319A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA970319A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q14194

Target Names

CRMP1

Alternative Names

Collapsin response mediator protein 1 antibody; CRMP 1 antibody; CRMP-1 antibody; Crmp1 antibody; Dihydropyrimidinase like 1 antibody; Dihydropyrimidinase related protein 1 antibody; Dihydropyrimidinase-related protein 1 antibody; DPYL1_HUMAN antibody; DPYSL1 antibody; DRP 1 antibody; DRP-1 antibody; DRP1 antibody; ULIP-3 antibody; Ulip3 antibody; Unc-33-like phosphoprotein 3 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CRMP1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17E7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CRMP1, also known as dihydropyrimidinase-related protein 1, plays a fundamental role in neuronal development and axon guidance. As a member of the collapsin response mediator protein family, CRMP1 mediates semaphorin signaling pathways that direct growth cone collapse and neuronal migration during nervous system formation. Beyond development, CRMP1 has emerged as a protein of interest in neurodegenerative disease research and studies of neuronal plasticity, making reliable detection tools essential for investigators working in these areas.

This recombinant monoclonal antibody, generated from clone 17E7, offers the consistency and reproducibility that demanding neuroscience research requires. Because the antibody sequence is defined and produced recombinantly in rabbit host, you can expect uniform performance across experiments and between lot numbers, eliminating a common source of variability in longitudinal studies. The monoclonal nature ensures recognition of a single epitope on the CRMP1 protein, providing specificity that supports confident data interpretation.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis detects CRMP1 at the expected 62 kDa molecular weight in both human SH-SY5Y neuroblastoma cells and mouse brain tissue lysates, confirming cross-species reactivity and suitability for comparative studies. Immunohistochemistry on paraffin-embedded human brain tissue reveals clear staining patterns, while flow cytometry analysis in SH-SY5Y cells shows distinct positive signal separation from isotype controls, enabling quantitative assessment of CRMP1 expression at the single-cell level.

Whether investigating axon guidance mechanisms, characterizing neuronal differentiation, or exploring CRMP1 dysregulation in neurological disorders, this antibody provides the versatility and reliability needed to advance your research with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance. During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone. Involved in invasive growth and cell migration. May participate in cytokinesis.

Gene References into Functions

Study provides a novel insight into the relevance of collapsin response mediator protein 1, a key molecule in semaphorin-3A signaling during neurodevelopment, in the molecular mechanism of action of lithium, and in the pathophysiology of bipolar disorder. PMID: 29666369
Study shows that CRMP1 acts an EMT and metastasis suppressor in prostate cancer cells via its regulation of actin polymerization. PMID: 27321179
Identify CRMP-1 as a novel regulator of actin filament elongation and reveal a surprisingly important role for CRMP-1, EVL, and actin polymerization in maintaining the structural integrity of epithelial sheets. PMID: 28630144
The present study reports that miR-187 was significantly overexpressed in gastric cancer tissues compared to that in non-tumor tissues and was associated with malignant clinical factors such as depth of invasion (P = 0.005), tumor size (P = 0.024), lymph node metastasis . It was found that collapsin response mediator protein 1 (CRMP1), a tumor suppressor, was a direct downstream target of miR-187. PMID: 27864146
miR200a-3p promotes the proliferation of human esophageal cancer cells by post-transcriptionally regulating CRMP1. PMID: 28025999
CRMP1 interacted with Spy1 which would disturb the association of CRMP1 with actin and was involved in the collapse of growth cones induced by Sema3A and regeneration after sciatic nerve crush. PMID: 25526860
These results demonstrate an important role for CRMP-1 in Listeria actin comet tail formation and open the possibility that CRMP-1 controls cell motility by modulating Arp2/3 activation. PMID: 26598519
Human CRMP-1 comprises a tetrameric assembly. PMID: 26249678
Results show that expression of CRMP1 is downregulated in medulloblastoma (MB) tumors. its overexpression inhibits proliferation, migration and invasion of cells suggesting that CRMP1 is implicated in MB pathogenesis. PMID: 26009886
Aberrant upregulation of CRMP-1 expression may link to the mechanism of developing early-onset preeclampsia. PMID: 25194153
CRMP1 targets aggregation-prone, N-terminal HTT fragments and suppresses their spontaneous self-assembly. PMID: 25908449
The mitochondrial fragmentation protein DRP1 play a role in the development of malignant melanoma. PMID: 26032958
in pilocarpine-induced animal model of epilepsy, CRMP-1 dynamically decreased in a range of 2 months. Thus, our results indicate that CRMP-1 may be involved in the development of TLE PMID: 22359051
CRMP1 is a novel candidate protein for schizophrenia traits PMID: 22798627
GSK3beta-dependent phosphorylation of LCRMP-1 provides an important mechanism for regulation of LCRMP-1 on cancer cell invasiveness and clinical outcome. PMID: 22363707
LCRMP-1 and CRMP-1 have opposing functions in regulating cancer cell invasion and metastasis PMID: 21747164
Data demonstrate that CRMP1 tyrosine 504 is a primary target of the Src family of tyrosine kinases (SFKs), specifically Fyn. PMID: 20506281
LCRMP-1 was a cancer invasion enhancer that could be a novel prognostic biomarker in non-small cell lung cancer. PMID: 19362386
CRMP-1 and CRMP-2 have a role in RhoA-dependent signaling, through interaction with and regulation of ROKalpha PMID: 12482610
Collapsin response mediator protein-1: a novel invasion-suppressor gene. PMID: 12650609
CTGF inhibits metastasis and invasion of human lung adenocarcinoma by a CRMP-1-dependent mechanism. PMID: 14996858
CRMP1 and EVC genes are located near WFS1, the Wolfram syndrome type 1 gene. PMID: 15492864
The CRMP1 protein is characterizing as modulators of Wnt protein signaling of Axin-1. PMID: 16169070
GSK3 alters phosphorylation of CRMP-1, -2, and -4 isoforms PMID: 16611631
The collapsin response mediator protein 1 and the promyelocytic leukemia zinc finger protein interact with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE). PMID: 17118363
Transcription of the invasion suppressor, CRMP-1, is reciprocally regulated at the promoter region by C/EBPalpha and Sp1. PMID: 18524846
ChIP and EMSA demonstrated that p50 binds to a kappaB site residing between -1753 and -1743 of the CRMP-1 promoter region. PMID: 18782567
loss of CRMP1 contribute to the increased invasive phenotype of human Glioblastoma multiforme brain tumors expressing mutant EGFRvIII. PMID: 19903856

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cell projection, growth cone. Cytoplasm, cytoskeleton. Perikaryon.

Protein Families

Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family

Tissue Specificity

Brain.

Database Links

HGNC: 2365

OMIM: 602462

KEGG: hsa:1400

STRING: 9606.ENSP00000321606

UniGene: Hs.135270

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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