CTBP2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA257842A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:A549 whole cell lysate(30µg), HT29 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HELA whole cell lysate(30µg), U251 whole cell lysate(30µg), A431 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain lysate(30µg) All lanes: CtBP2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time：10s IHC image of CSB-RA257842A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA257842A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA257842A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA257842A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P56545

Target Names

CTBP2

Alternative Names

C terminal binding protein 2 antibody; C-terminal-binding protein 2 antibody; CtBP2 antibody; CTBP2_HUMAN antibody; ribeye antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human CTBP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

C-terminal binding protein 2 (CTBP2) serves as a transcriptional corepressor with critical roles in developmental processes and cellular differentiation. This protein functions as a key regulator of gene expression by interacting with sequence-specific DNA-binding proteins, and its dysregulation has been implicated in various pathological conditions, making it a valuable target for researchers investigating transcriptional control mechanisms and disease biology.

This recombinant monoclonal antibody against CTBP2 offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, clone 8D8 delivers the lot-to-lot reproducibility essential for longitudinal studies and multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human CTBP2, has been affinity-purified to ensure high specificity for your target.

Extensive validation across multiple platforms demonstrates this antibody's versatility in your research applications. Western blot analysis confirms detection of the expected 49 kDa band across diverse human cell lines including A549, HT29, COLO205, HeLa, U251, and A431, with cross-species reactivity validated in mouse and rat brain tissue lysates. Immunohistochemistry studies have successfully visualized CTBP2 in paraffin-embedded human lung tissue and breast cancer specimens using citrate buffer antigen retrieval. Immunofluorescence staining in HeLa cells reveals clear subcellular localization patterns, while flow cytometry analysis demonstrates robust detection suitable for single-cell studies.

The broad application compatibility spanning western blot, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA provides researchers with flexibility to investigate CTBP2 across multiple experimental contexts, from protein expression analysis to tissue localization studies in cancer and developmental biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.; Isoform 2 probably acts as a scaffold for specialized synapses.

Gene References into Functions

the present study indicated that CtBP2 reduced the susceptibility of ECA109 cells to cisplatin by regulating the expression of apoptosis-related proteins, suggesting that it may be a promising therapeutic target in esophageal squamous cell carcinoma in the future. PMID: 29658564
Results indicated that CTBP2 was direct target of miR-338-5p in glioma cells. CTBP2 silencing can rescued the phenotype changes induced by miR-338-5p inhibitor on cell proliferation and invasion in glioma. PMID: 28826173
this study shows that CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis PMID: 28404932
these findings provide insight into the role CtBP2 plays in promoting proliferation and migration in breast cancer by the inhibition of p16INK4A. PMID: 28412731
CtBP2 ameliorated palmitic acid-induced insulin resistance via ROS-dependent JNK pathway. PMID: 28111233
CtBP2 is a druggable transforming oncoprotein critical for the evolution of neoplasia driven by Apc mutation. PMID: 28414304
CtBP2 may be a potential target to suppress tumorigenesis in neuroblastoma PMID: 28179207
This Gene-based tests suggest evidence of association with related genes, ZEB2, RND3, MCTP1, CTBP2, and beta EEG PMID: 28040410
Our results indicated that CCNH/CDK7-CtBP2 axis may augment ESCC cell migration, and targeting the interaction of both may provide a novel therapeutic target of esophageal squamous cell carcinoma . PMID: 25820824
Releasing the key adipogenic regulator C/EBPalpha from CtBP2 binding. PMID: 25895816
Enhanced CtBP2expression promoted HCC xenograft growth and induced EMT. PMID: 25686837
results show how CtBP2 contributes to prostate cancer progression by modulating AR and oncogenic signaling PMID: 25228652
High CTBP2 expression is associated with prostate cancer. PMID: 24332637
Overexpression of CtBP2 is associated with breast carcinoma. PMID: 24522810
CtBP2 might contribute to the progression of esophageal squamous cell carcinoma through a negative transcriptional regulation of p16(INK4A). PMID: 23255392
CtBP2 is over-expressed in prostate cancer.CtBP2 promotes prostate cancer cell proliferation through c-Myc signaling. PMID: 24835310
BRCA1 expression is epigenetically repressed in sporadic ovarian cancer cells by overexpression of C-terminal binding protein 2.CtBP2 is an ovarian cancer oncogene. PMID: 23730208
CtBP2 directly targeted stem cell core genes resulting in increased cancer cell stemness and increasing metastatic and tumorigenic potential. PMID: 24012420
E2F7 recruits the co-repressor C-terminal-binding protein (CtBP) and that CtBP2 is essential for E2F7 to repress E2F1 transcription. PMID: 23853115
CTBP2 is a transcriptional cofactor for RXR-alpha/RAR-alpha. PMID: 23775127
We propose that CtBP2 is an ovarian cancer oncogene that regulates gene expression program by modulating HDAC activity. PMID: 22945647
these data demonstrate that CHIP regulates the steady-state level of CtBP2 as an E3 ubiquitin ligase and determines the expression levels of CtBP2 target genes. PMID: 23410750
Interaction with cyclin H/cyclin-dependent kinase 7 (CCNH/CDK7) stabilizes C-terminal binding protein 2 (CtBP2) and promotes cancer cell migration PMID: 23393140
Doubly transgenic zebrafish exhibit a startle response and typical swimming behavior, indicating that there is no gross disruption of either hearing or vestibular function useful in the study of ribbon synapse development of the hair cell. PMID: 21334379
This study demonstrates that ataxin-1 occupies the promoter region of E-cadherin in vivo and that ataxin-1 activates the promoter in a CtBP2-mediated transcriptional regulation manner. PMID: 21315774
we demonstrate that it is the interaction of CtBPs with transcriptional regulators and/or chromatin-modifying enzymes in the cell nucleus, rather than their role in Golgi fission, which is critical for the maintenance of mitotic fidelity. PMID: 21057548
CtBP2 proteins are ubiquitously expressed in all lines and tumour samples. PMID: 20964627
CtBP2 selectively down-regulates Th2 cytokines, therefore it is a potential target for the treatment of allergic diseases. PMID: 20523059
Data suggest that ARF antagonism of CtBP repression of Bik and other BH3-only genes may have a critical role in ARF-induced p53-independent apoptosis and tumor suppression. PMID: 19798104
roles of acetylation in regulating subcellular localization and transcriptional activity of CtBP2. PMID: 16356938
analysis of the CtBP2 corepressor complex induced by E1A and modulation of E1A transcriptional activity by CtBP2 PMID: 17023432
E1A may gain access to cellular promoters through conservved sequence-dependent interaction with CtBP2. PMID: 17546044
Study shows that transcription corepressor CtBP2 directly binds acinus, which is regulated by nerve growth factor (NGF), inhibiting its stimulatory effect on cyclin A1, but not cyclin A2, expression in leukemia. PMID: 19668232
CtBP2 monomer interacts with a major CtBP-dependent repressor ZEB and HDAC and that the interaction of the two factors with the CtBP2 monomer was mutually exclusive. PMID: 19754958
Genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 18264096

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Subcellular Location

Nucleus. Cell junction, synapse.

Protein Families

D-isomer specific 2-hydroxyacid dehydrogenase family

Tissue Specificity

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

Database Links

HGNC: 2495

OMIM: 602619

KEGG: hsa:1488

STRING: 9606.ENSP00000311825

UniGene: Hs.501345

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Notch signaling pathway Wnt signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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