Product Details

Uniprot No.

O60716

Target Names

CTNND1

Alternative Names

Catenin delta-1 (Cadherin-associated Src substrate) (CAS) (p120 catenin) (p120(ctn)) (p120(cas)), CTNND1, KIAA0384

Species Reactivity

Human

Immunogen

A synthesized peptide from human CTNND1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6F11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CTNND1, also known as p120 catenin or catenin delta-1, serves as a critical regulator of cell-cell adhesion and epithelial integrity. This armadillo repeat protein stabilizes cadherin complexes at adherens junctions and modulates Rho GTPase signaling, making it essential for understanding epithelial homeostasis, cancer progression, and metastatic potential. Alterations in CTNND1 expression or localization have been implicated in various malignancies, positioning it as a valuable marker for researchers investigating tumor biology and cellular adhesion dynamics.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human CTNND1, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target.

Validation across multiple platforms demonstrates genuine experimental flexibility. Immunohistochemistry studies in paraffin-embedded human cervical cancer and placenta tissues at 1:100 dilution reveal clear target detection using standard citrate buffer antigen retrieval protocols. Immunofluorescence analysis in A549 lung adenocarcinoma cells shows distinct cellular localization patterns, while flow cytometry validation in BxPC-3 pancreatic cancer cells confirms robust detection of intracellular CTNND1 following permeabilization.

Whether you are examining cadherin-catenin complex dynamics, investigating epithelial-mesenchymal transition mechanisms, or characterizing adhesion molecule expression in tumor samples, this antibody provides a reliable tool for multiple detection platforms. Its demonstrated performance across both tissue sections and cultured cell lines supports diverse experimental approaches in cancer biology and cell adhesion research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.

Gene References into Functions

pathogenic variants are described in four genes encoding components of the p120-catenin complex (CTNND1, PLEKHA7, PLEKHA5) and an epithelial splicing regulator (ESRP2), in addition to the known Cleft lip/Palate-associated gene, CDH1, which encodes E-cadherin. PMID: 29805042
CTNND1 binds to CDH1 and controls the stability of the complex. PMID: 29348693
miR-298 suppresses hepatocellular carcinoma progression at least partially by targeting CTNND1-mediated Wnt/beta-catenin signaling. PMID: 29990836
these data indicated under cigarette smoke condensate treatment; losing of membrane p120ctn could upregulate surface NEP protein level and thus facilitate BEAS-2B cell migration. PMID: 30249887
Data show that p120-catenin interacts with kinesin family member 23 (MKLP1) to regulate focused rhoA GTP-binding protein (RhoA) activity during cytokinesis. PMID: 28004812
Results provide evidence that 90K interacts with the E-cadherin-p120-catenin complex and induced its dissociation, altering the phosphorylation status of p120-catenin. PMID: 29207493
Results show that head and neck squamous cell carcinoma tumors with low P120CTN and PI3K pathway mutations have higher levels of MMP1 compared to tumors with high P120CTN and no PI3K pathway mutations demonstrating that P120CTN downregulation and PIK3CA mutations promote MMP1-driven invasion. PMID: 28637905
In 11 BCD patients from eight families, we identified five CDH1 deleterious missense mutations and three CTNND1 truncating mutations. PMID: 28301459
Our combined data indicate that as HPMECs achieve confluence and CD31 ectodomains become homophilically engaged, multiple SFKs are activated to increase tyrosine phosphorylation of p120ctn, which in turn, functions as a cross-bridging adaptor molecule that physically couples NEU1 to CD31, permitting NEU1-mediated desialylation of CD31. PMID: 28343945
Studied interactions between protein kinase C alpha (PKCalpha), FOXC2, and p120-catenin (CTNND1) in breast cancer, cell migration/ invasion; found PKCalpha acts as an upstream regulator of FOXC2, which in turn represses the expression of p120-catenin, in both in endocrine resistant ER+breast cancer and basal A triple negative breast cancer PMID: 29216867
Studied the association between genetic polymorphisms in the CTNND1 gene and risk of pancreatic carcinoma in Chinese population. PMID: 27565611
Results found that CTNND1 expression was significantly up-regulated in hepatocellular carcinoma (HCC) cancer lesions compared with paired normal liver tissues and, could promote cell proliferation, migration, and invasion in vitro and in vivo. The study provides evidence that CTNND1 functions as a novel tumor oncogene in HCC. PMID: 27193094
These results suggest that stabilization of delta-catenin by Hakai is dependent on Src. PMID: 28069439
These results uncover a new role for p120 catenin bound to the N-cadherin precursor ensuring its trafficking through the biosynthetic pathway towards the cell surface. PMID: 27254316
recent results describing actions of p120-catenin in different phases of this pathway PMID: 28276699
The mTOR-dependent, epithelial phenotype of TSC astrocytes suggests TSC1/2 and mTOR tune the phosphorylation level of catenin delta-1 by controlling PKCe activity, thereby regulating the mesenchymal-epithelial-transition (MET) PMID: 27516388
Src-dependent phosphorylation of p120(ctn) can respond rapidly to negative pressure and contribute to E-cadherin downregulation. PMID: 27220534
p120 participates in the progress of gastric cancer through regulating Rac1 and Pak1. PMID: 26324182
The overexpression of P120ctn led to a decrease in both invasion and migration capacity of HN12 cells accompanied by a decrease in EMT markers. Knockdown of P120ctn led to an increase in both invasion and migration capacity accompanied by an increase in EMT markers. PMID: 27146732
OGT inhibited the formation of the Ecadherin/catenin complex through reducing the interaction between p120 and Ecadherin. PMID: 26707622
Immunohistochemical panel of CDX2, p120ctn, c-Myc, and Jagged1 proteins would be to distinguish between low/high grade dysplasia in histologically challenging cases of Barrett's esophagus. PMID: 26926447
p120ctn improves the BBB dysfunction and inflammatory responses induced by LPS through the inhibition of NF-kappaB activation. PMID: 26097613
expression of catenin-delta1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion PMID: 26992637
expression of E-cadherin, and p120 negatively correlated with the tumor differentiation of oral squamous cell carcinoma. PMID: 26464646
Survival time of colorectal cancer patients with positive deltacatenin expression was shorter than that of patients with negative deltacatenin expression. PMID: 26062780
results indicate an anti-inflammatory effect of p120 in bronchial epithelial cells through its modulation of NF-kappaB signaling depending on RhoA/ROCK pathway. PMID: 25693631
Phosphorylation and isoform use in p120-catenin during development and tumorigenesis. PMID: 26477567
PLEKHA7 localization to adherens junctions is E-cadherin and p120 dependent. PMID: 26302406
Pro-Tumorigenic Phosphorylation of p120 Catenin is associated with Renal and Breast Cancer. PMID: 26067913
the modulation of HPV-16 E6/E7 expression remarkably influenced cell proliferation, migration, and invasion, as well as the protein levels of E-cadherin and P-cadherin in cervical cell lines. PMID: 26093522
The expression, redistribution and disassociation of junction proteins in ventilator-induced lung injury were all restored with p120-catenin overexpression. PMID: 25986488
localization of p120 catenin in the cytoplasm rather than the membrane correlates with poor prognosis in esophageal squamous cell carcinoma PMID: 25785604
Enforced expression of miR-29s in gastric cancer cells inhibited cell invasion in vitro and in vivo by directly targeting CTNND1. PMID: 25634213
C6orf106 promotes invasion in NSCLC cells. Finally, C6orf106 upregulates vimentin, and downregulates E-cadherin and P120ctn. PMID: 25736925
p120ctn down-regulation and EGFR overexpression are able to mimic human ESCC in a culture model PMID: 25529795
MiR-145 inhibits invasion of gastric cancer cells not only by down-regulating cytoplasmic catenin-delta1 expression but also by inducing the translocation of catenin-delta1 PMID: 25470111
We conclude that delta-catenin tends to overexpress in breast carcinoma and promotes the malignant phenotype PMID: 25273174
uncovering a critical role for CTNND2 in neuronal development and an intimate connection to chromatin biology; data contribute to the understanding of the genetic architecture of autism PMID: 25807484
our study supports the regulatory role of p120 in airway inflammation and reveals that p120 may modulate NF-kappaB signaling partially through RhoA. PMID: 24995336
E-cadherin and P120 catenin cocktail immunostain can be used to differentiate ductal carcinoma in situ from lobular carcinoma in situ. PMID: 24966968
Overexpression of delta-catenin reduces the expression of E-cadherin and alters the balance between E-cadherin and p120ctn, which in turn affects the formation of intercellular adhesions and promotes invasion and metastasis in Colorectal cancer. PMID: 23423910
Transfection of in H1299 cells expressing low p120ctn levels. PMID: 24505377
these data suggest that PTP-PEST affects epithelial cell motility by controlling the distribution and phosphorylation of p120 and its availability to control Rho GTPase activity PMID: 24284071
P120ctn plays a pivotal role in the proliferation. PMID: 23073801
Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene. PMID: 23981612
P120 catenin ARM domains 1, 3-5, and 8 mediate interactions between p120 catenin and MUC1. PMID: 24371222
The optimized knockdown with p120 and Kaiso siRNAs further expands the size of HCEC monolayers without endothelial mesenchymal transition (EMT) via selective activation of p120/Kaiso signaling that requires the RhoA-ROCK-noncanonical BMP-NFkB signaling. PMID: 24474278
these results indicate that c-Src can enhance the oncogenic function of delta-catenin in prostate cancer cells PMID: 24412473
Overexpression of NLBP promotes the cell proliferation of lung adenocarcinoma through interacting with p120ctn. PMID: 23839039
p120ctn delocalization/loss of expression could be an independent prognostic marker in oral squamous cell carcinoma PMID: 23706919

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Involvement in disease

Blepharocheilodontic syndrome 2 (BCDS2)

Subcellular Location

Cell junction, adherens junction. Cytoplasm. Nucleus. Cell membrane.; [Isoform 1A]: Nucleus.; [Isoform 2A]: Nucleus.; [Isoform 3A]: Nucleus.

Protein Families

Beta-catenin family

Tissue Specificity

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and gener

Database Links

HGNC: 2515

OMIM: 601045

KEGG: hsa:1500

STRING: 9606.ENSP00000382004

UniGene: Hs.166011

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Code	CSB-RA268308A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA268308A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA268308A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA268308A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing BxPC-3 cells stained with CSB-RA268308A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CTNND1 Recombinant Monoclonal Antibody

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