Product Details

Uniprot No.

Target Names

CTTN

Alternative Names

Amplaxin antibody; CTTN antibody; EMS 1 antibody; EMS1 antibody; FLJ34459 antibody; Mammary tumor and squamous cell carcinoma associated antibody; Oncogene EMS1 antibody; p80/85 src substrate antibody; Src substrate cortactin antibody; SRC8_HUMAN antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human CTTN

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cortactin, encoded by the CTTN gene, serves as a critical actin-binding protein that orchestrates cytoskeletal dynamics at the leading edge of migrating cells. As a key substrate of Src kinase, cortactin plays essential roles in cell motility, invasion, and membrane trafficking processes. Its frequent overexpression in various carcinomas, including breast and head and neck cancers, has established cortactin as an important marker in cancer biology research, particularly in studies investigating metastatic potential and tumor progression.

This recombinant monoclonal antibody offers the reproducibility that demanding research requires. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format, produced from clone 13E1 against a synthetic peptide derived from human cortactin, delivers consistent performance across your experimental timeline.

Validation data demonstrates robust detection across multiple platforms and species. Western blot analysis confirms specific recognition of the expected 80 kDa band in diverse human cell lines including HeLa, A549, COLO-205, HT-29, PC-3, and U251-MG, as well as mouse NIH/3T3 cells, supporting the cross-species reactivity with human, mouse, and rat samples. Flow cytometry validation in HeLa cells shows clear separation from isotype control, enabling intracellular protein quantification in single-cell analyses. The antibody also performs in ELISA applications, providing flexibility across detection platforms.

Whether investigating cytoskeletal remodeling, Src signaling pathways, or tumor cell invasion mechanisms, this antibody provides a reliable tool for cortactin detection in your cancer biology and cell migration research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane.

Gene References into Functions

Cortactin (CTTN) silencing in megakaryocyte (MK) phenocopies histone deacetylase 6 (HDAC6) inactivation and knockdown leads to a strong proplatelet formation (PPF) defect. PMID: 29176689
Cortactin expression in carcinoma cells and its known involvement in the EGFR pathway suggest a role for this protein as a target for laryngeal squamous cell carcinoma therapy. PMID: 28885660
Cortactin depletion in HMEC-1 cells results in increased stress fibre contractility and endothelial barrier destabilisation. Secretion of the barrier-stabilising hormone adrenomedullin, which activates Rap1 and counteracts actomyosin contractility, was reduced in supernatants of cortactin-depleted endothelium. Cortactin acts in controlling actomyosin contractility with consequences for endothelial barrier integrity. PMID: 27357373
Data show that cortactin-mediated p21Cip1 nuclear export and degradation facilitating MCP1-induced human aortic smooth muscle cell (HASMC) proliferation. PMID: 27363897
Mena(INV) promotes invadopodium maturation by inhibiting normal dephosphorylation of cortactin at tyrosine 421 by the phosphatase PTP1B. PMID: 27824079
CTTN expression increases EGFR protein levels and enhances the activation of the MAPK signaling pathway. CTTN expression also inhibits the ubiquitin-mediated degradation of EGFR by suppressing the coupling of c-Cbl with EGFR. PMID: 27903975
the study revealed that PTBP1 facilitates colorectal cancer migration and invasion activities by inclusion of cortactin exon 11 PMID: 28404950
Overall, the authors find that p27 directly promotes cell invasion by facilitating invadopodia turnover via the Rac1/PAK1/Cortactin pathway. PMID: 28287395
Tyrosine dephosphorylation of the cytoskeletal scaffold, cortactin, recruits the RhoA antagonist SRGAP1 to relax adherens junctions in response to HGF. PMID: 28983097
cortactin binds to E-cadherin, and that a posttranslational modification of cortactin, RhoA-induced phosphorylation by protein kinase D1 (PKD1; also known as PRKD1) at S298, impairs adherens junction assembly and supports their dissolution. PMID: 27179075
Unique role for PBF in regulating CTTN function to promote endocrine cell invasion and migration. PMID: 27603901
AMPK phosphorylation of cortactin followed by SIRT1 deacetylation modulates the interaction of cortactin and cortical-actin in response to shear stress. Functionally, this AMPK/SIRT1 coregulated cortactin-F-actin dynamics is required for endothelial nitric oxide synthase subcellular translocation/activation and is atheroprotective. PMID: 27758765
These data suggest a model in which cortactin promotes exosome secretion by stabilizing cortical actin-rich multivesicular late endosome docking sites. PMID: 27402952
Cortactin may have an important role in the development of oral tumors in human PMID: 27148699
a dynamic TIP150-cortactin interaction orchestrates directional cell migration via coupling dynamic microtubule plus ends to the cortical cytoskeleton. PMID: 27451391
Amplification of 11q13 resulting in overexpression of CTTN/CCND1 was the most prominent finding, which was observed in 13 of 19 ESCC cases. PMID: 27877079
The cortactin Tyr421 residue is required to promote cell proliferation both in vitro and in vivo. PMID: 27805253
the expression of CTTN, Exo70 and MMP-9 in HCC cells was detected and their relations with the ability of migration and invasion of hepatoma carcinoma cells were evaluated PMID: 27025610
miR-542-3p inhibits the invasion of colorectal cancer cells by targeting CTTN. PMID: 26952924
Cortactin expression in epithelial ovarian carcinoma was closely related to malignant biological behavior PMID: 26243395
the MCP1-induced cortactin phosphorylation is dependent on PLCb3-mediated PKC activation, and siRNA-mediated down-regulation of either of these molecules prevents cortactin interaction with WAVE2 PMID: 26490115
findings reveal that Keap1 regulates cell migration by affecting the subcellular localization and activity of cortactin independently of its role in oxidant stress responses. PMID: 26602019
Host cortactin, PKD1 and actin are recruited by Trypanosoma cruzi extracellular amastigotes based on experiments in fixed and live cells by time lapse confocal microscopy. PMID: 26096820
Study highlights the potential role of CCM3 in regulating tight junction complex organization and brain endothelial barrier permeability through CCM3-ERK1/2-cortactin cross-talk PMID: 26385474
We identified and confirmed that Fra-1 affected the expression level of CTTN and EZR in vitro through LC-MS/MS analyses and western blot technology. PMID: 26330014
acetylation-mimicking mutants of alpha-tubulin and cortactin counteract HDAC6-induced ciliary disassembly. PMID: 26246421
cortactin is crucial in invasion and migration of glioma cells, which may promote the migration and invasion of glioma cells by regulating lamellipodia formation, a process requiring the combination of cortactin and the Arp2/3 complex PMID: 26238396
Findings show CTTN significantly upregulated in colon cancer, while its ectopic expression promotes cell proliferation and tumorigenicity through EGFR-ERK pathway regulation suggesting that CTTN plays an important role in colon cancer progression. PMID: 26151562
The results indicate that cortactin is involved in the regulation of apoptosis induced by VacA in gastric cells. PMID: 26289258
Studied the expression of cortactin in HCT116 cells treated with one of the major components of Chansu, Cinobufagin. PMID: 26134506
These findings suggest that this common cortactin variant may functionally contribute to ALI predisposition by impeding endothelial wound healing. PMID: 26361873
These data suggest a model in which phosphatidylinositol 3,5-bisphosphate binding removes cortactin from late endosomal branched actin networks and thereby promotes net actin turnover. PMID: 26323691
Dysregulation of cortactin and HDAC6 is implicated in the invasiveness and migration of prostate cancer cells. PMID: 26112958
These results implicate that the role of MTSS1 suppresses cell migration and invasion by inhibiting expression of CTTN and as a prognosis biomarker in Glioblastomas PMID: 25385572
Upregulation of CTTN is critical for Vascular endothelial growth factor-C- mediated tumor growth and metastasis of esophageal squamous cell carcinoma. PMID: 25212831
The cortactin SH3 domain contributes to podosome assembly while fascin actin bundling is a master regulator of podosome disassembly in THP-1 macrophages and dendritic cells. PMID: 25601713
Integrated omic analysis of oropharyngeal carcinomas reveals human papillomavirus-dependent regulation of the AP-1 pathway and elevated cortactin protein levels. PMID: 25271301
This review focuses on recently published evidence that endothelial ABPs, such as cortactin, myosin, or alpha-actinin, regulate leukocyte extravasation by controlling actin dynamics, biomechanical properties of endothelia, and signaling pathways PMID: 25848070
c-src-mediated phosphorylation requires RTPAalpha PMID: 24652832
Cortactin, another player in the Lyn signaling pathway, is over-expressed and alternatively spliced in leukemic cells from patients with B-cell chronic lymphocytic leukemia. PMID: 24532043
Cortactin expression promoted the migration, invasion and proliferation of SGC-7901 cells both in vivo and in vitro PMID: 24696610
These findings show that overexpression of PODXL enhanced invadopodia formation and tumor metastasis by inducing Rac1/Cdc42/cortactin signaling network. PMID: 24970760
Mechanistic investigations showed that VEGF-C increased CTTN expression by downregulating Dicer-mediated maturation of miR326, thereby relieving the suppressive effect of miR326 on CTTN expression. PMID: 25205106
association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700464
Data suggest that curcumin is an activator of non-receptor type 1 protein tyrosine phosphatase (PTPN1) and can reduce cell motility in colon cancer via dephosphorylation of pTyr(421)-cortactin (CTTN). PMID: 24465712
Overexpression of cortactin (CTTN) was observed in 126/198 (63.6%) of esophageal squamous cell carcinoma cases and was significantly associated with lymph node metastasis (P = 0.000), pathologic stage (P = 0.000) and poor survival (P<0.001). PMID: 24551190
The present study demonstrated that bladder cancer cells with cortactin knockdown have a reduced capacity to extravasate into the lung from the circulation, due to the decreased invasive character of invadopodia. PMID: 24549769
Studies show that IQGAP1 functions as a hub linking HGF-induced signaling to microtubules and actin remodeling via EB1-IQGAP1-cortactin interactions. PMID: 25022754
expression of a charge-neutralizing cortactin mutant inhibited contraction and actin dynamics during contractile activation. PMID: 24920679
Two amino acid residues confer different binding affinities of Abelson family kinase SRC homology 2 domains for phosphorylated cortactin. PMID: 24891505

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Subcellular Location

Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, dendrite. Cell projection. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, podosome. Cell junction. Cell junction, focal adhesion. Membrane, clathrin-coated pit. Cell projection, dendritic spine. Cytoplasm, cell cortex.

Database Links

HGNC: 3338

OMIM: 164765

KEGG: hsa:2017

UniGene: Hs.596164

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Code	CSB-RA548109A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(20µg), NIH/3T3 whole cell lysate(20µg), A549 whole cell lysate(20µg), COLO-205 whole cell lysate(20µg), HT-29 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), U251-MG whole cell lysate(20µg) All lanes: Src substrate cortactin antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time：1min Overlay Peak curve showing Hela cells stained with CSB-RA548109A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CTTN Recombinant Monoclonal Antibody

Product Details

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Target Background