DCTN3 Recombinant Monoclonal Antibody

DCTN3, also known as dynactin subunit 3 or p22, serves as an essential component of the dynactin complex, a multiprotein assembly that plays a critical role in cytoplasmic dynein-mediated transport processes. This 22 kDa subunit contributes to the structural integrity of the dynactin complex, facilitating the movement of organelles, vesicles, and chromosomes along microtubules during cell division and intracellular trafficking. Understanding DCTN3 function provides valuable insights into cellular transport mechanisms and their dysregulation in various pathological conditions.

This recombinant monoclonal antibody, clone 3D5, offers researchers the reproducibility and consistency that comes with sequence-defined production. Generated against a synthetic peptide derived from human DCTN3, the rabbit host-derived IgG undergoes affinity chromatography purification to ensure high specificity for your target. The recombinant format eliminates the lot-to-lot variability often encountered with traditional hybridoma-produced antibodies, allowing you to maintain experimental consistency across long-term studies.

Validation data demonstrates reliable performance across multiple detection platforms. Immunohistochemistry staining has been successfully performed in paraffin-embedded human brain tissue and human endometrial cancer samples at 1:100 dilution using citrate buffer antigen retrieval, revealing clear target localization. Immunofluorescence studies in A-431 cells show distinct staining patterns when used at 1:50 dilution, while flow cytometry analysis in HeLa cells confirms robust detection with clear separation from isotype controls.

This versatility across tissue sections, adherent cell lines, and suspension-based flow cytometry applications makes this antibody a practical choice for cell biology research investigating intracellular transport, mitotic processes, and dynactin complex function in both normal and disease contexts.