Product Details

Uniprot No.

Target Names

DISC1

Alternative Names

C1orf136 antibody; DISC1 antibody; DISC1_HUMAN antibody; Disrupted in schizophrenia 1 antibody; Disrupted in schizophrenia 1 protein antibody; KIAA0457 antibody; RP4-730B13.1 antibody; SCZD9 antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human DISC1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7E11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Disrupted in schizophrenia 1 (DISC1) represents one of the most compelling risk factors for major psychiatric disorders, with genetic disruptions linked to schizophrenia, bipolar disorder, and major depression. This multifunctional scaffolding protein plays essential roles in neurodevelopmental processes including neuronal migration, neurite outgrowth, and synaptic regulation, making it a critical target for researchers investigating the molecular underpinnings of mental illness and brain development.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human DISC1, offers the reproducibility and consistency that demanding neuroscience research requires. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring your experimental conditions remain constant across projects spanning months or years.

Validation studies demonstrate reliable performance across multiple experimental platforms. In western blot applications, the antibody detects DISC1 in diverse human cell lines including HeLa, U-251MG glioblastoma, HEK293T, HL-60, and SW620 cells, as well as rat brain tissue lysate, confirming cross-species reactivity valuable for translational studies. The observed 115 kDa Western Blot band (larger than the predicted 100 kDa) for the recombinant monoclonal anti-DISC1 antibody reflects recognition of the actual expressed DISC1 isoform—either a longer variant via alternative splicing (inserting extra exons) or a post-translationally modified (e.g., glycosylated) form—whose increased mass (115 kDa) exceeds the theoretical 100 kDa full-length/precursor size. Flow cytometry validation in Jurkat cells demonstrates clear positive signal separation from isotype controls, supporting intracellular detection applications.

Whether you are mapping DISC1 protein interaction networks, investigating its role in neural progenitor cell biology, or exploring psychiatric disease mechanisms, this antibody provides a dependable tool for advancing your understanding of this pivotal neurodevelopmental regulator.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Inhibits ATF4 transcription factor activity in neurons by disrupting ATF4 dimerization and DNA-binding. Plays a role, together with PCNT, in the microtubule network formation.

Gene References into Functions

Significant effect of truncated hDISC1 on glial identity cells along the rostrocaudal axis and their oligodendrocytes specification; appearance of hindbrain oligodendrocyte lineage cells and their premature differentiation may affect cerebrocortical organization and contribute to the pathophysiology of SZ. PMID: 28981898
Study shows that DISC1-mutant cerebral organoids display disorganized structural morphology and impaired proliferation, which is phenocopied by WNT agonism and rescued by WNT antagonism. PMID: 29643329
Results suggest that DISC1 may be involved in the regulation of lactate production in astrocytes to support neuronal activity and associated behaviors. PMID: 29643356
This study showed several gene expression profiles, including DISC1, are associated with age-related changes in White Matter disconnectivity. PMID: 28946750
DISC1 overexpression and misassembly is associated with deficits in long-term memory and attention-related behavior in a tgDISC1 rat model. PMID: 29107702
Findings showed that NOS1AP (rs348624, rs12742393 and rs1415263), DISC1 (rs821633 and rs1000731), DAOA (rs2391191) and GSK3B (rs6438552) SNPs had no association with development of early-onset schizophrenia; however, our finding suggested statistically significant role of the interaction of NOS1AP, DISC1, DAOA and GSK3B polymorphisms in schizophrenia susceptibility. PMID: 29100974
Meta-analysis found that DISC1 polymorphisms increased a risk of schizophrenia, especially in the Chinese population. PMID: 29031911
DISC1 a key molecular lead in psychiatry and neurodevelopment. PMID: 27595595
The nervous system developmental pathway is a potential pathogenesis of sporadic amyotrophic lateral sclerosis, among them, the polymorphism of rs3737597 in DISC1 might play some roles. PMID: 27052956
meta-analysis analyzed the association between DISC1 SNPs rs3738401 and rs821616 with schizophrenia. We found that rs3738401 did not show association with schizophrenia in the Caucasian, Asian or Japanese subgroups PMID: 29410289
DISC1 affects glioblastoma cell development via mitochondria dynamics partly by down regulation of Drp1. PMID: 27852062
In combination with molecular modeling, data support predictions regarding the three-dimensional fold of a DISC1 segment as well as its steric arrangement in complex with a llama heavy chain antibody. PMID: 29324815
Authors found that three pathways that include the homologs of Drosophila Dys, Trio, and Shot were downregulated by introducing a C-terminal truncated mutant DISC1. PMID: 28472294
disrupting DISC1/Ndel1 complex formation prolongs mitotic length and interferes with cell-cycle progression in human cells, and it causes cell-cycle deficits of radial glial cells in the embryonic mouse cortex and human forebrain organoids PMID: 29103808
Study reports sex-specific influence of common disrupted-in-schizophrenia-1 variants on volumes of the basal ganglia, the amygdala and on the cortical surface area. PMID: 27369464
Study found a significant association between the DISC1 gene polymorphism rs6675281 alone, and the combination of rs6675281 and rs821616, and differences in long-term cortical thickness growth in patients with a first-episode of psychosis; and observed an overall difference in cortical thickness, as well as heightened disparities in the frontal and temporal brain regions. PMID: 26209938
HTT forms a ternary protein complex with the scaffolding protein DISC1 and cAMP-degrading phosphodiesterase 4 (PDE4) to regulate PDE4 activity. PMID: 28263187
This theme highlights the importance of understanding precisely how DISC1 can regulate intracellular trafficking, and suggests that a novel approach to the treatment of psychiatric disorders could be provided by targeting this protein and the trafficking machinery with which it interacts. PMID: 27121900
Findings indicate that disrupted-in-schizophrenia 1 (DISC1) and close homolog of L1 may engage in physical and functional interaction in neural development, supporting the notion that DISC1 regulates neurite outgrowth with a receptor belonging to the neural cell adhesion molecules. PMID: 27346367
DISC1 increased the risk for late-onset Alzheimer's disease in northern Han Chinese population. PMID: 27023224
Findings suggest that synapse-associated protein of 97-kDa molecular weight and disrupted in schizophrenia 1 contribute to maintaining Wnt/beta-catenin signaling activity within a homeostatic range by regulating glycogen synthase kinase 3 beta phosphorylation. PMID: 27026592
DISC1 has distinctly folded regions, which are bisected by mental illness-related mutations PMID: 28249940
These results should contribute to reveal DISC1 physiological function and potential pathogenic role in severe mental illnesses PMID: 27466199
This population-based case-control study was carried out to determine whether polymorphisms in DISC1 and NRG1 genes could be associated with schizophrenia in the Chinese population. PMID: 27236031
This study demonstrated that the Increased density of DISC1-immunoreactive oligodendroglial cells in fronto-parietal white matter of patients with paranoid schizophrenia. PMID: 26315603
The present findings, at least in part, provide some clues for further investigating the association of DISC1 variants with SCZ susceptibility PMID: 26945459
DISC1 may be involved in sleep regulation PMID: 27354230
Polymorphisms in DISC1 play a role in vulnerability to opioid dependence in a Polish sample. PMID: 26997180
Hypoxic preconditioning decreases NF-kappaB activity via DISC1. PMID: 26615762
It is anterogradely transported to the neurite tips, together with Lis1, and functions in neurite extension via suppression of GSK3beta activity. PMID: 27333658
DISC1 disruption affects expression of neural cell fate markers and Wnt signaling. PMID: 26299970
These results uncover an unexpected role for DISC1 in normal beta-cell physiology and suggest that DISC1 dysregulation contributes to T2D independently of its importance for cognition. PMID: 26546129
DISC1 gene variations may affect the course of cognitive deficits found in patients suffering from the first episode of non-affective psychosis PMID: 26443054
DISC1 acts as an important regulator of mitochondrial dynamics in both axons and dendrites. PMID: 26553875
An association was found with DISC1 genetic variants and susceptibility to schizophrenia in a Han Chinese population. PMID: 26162659
Results with immature neurons from patients with schizophrenia and bipolar disorder showed common alterations in DISC1 levels and its cellular distribution, cAMP homeostasis, and cell migration PMID: 25620115
results demonstrate that DISC1 protein levels in human lymphocytes are correlated with the diagnosis of schizophrenia independent of smoking and thus present a potential biomarker PMID: 25218871
The results of this student findings provide consistent evidence that the DISC1 Ser704Cys polymorphism influences the thalamic-prefrontal circuits in humans. PMID: 24146131
Transient neonatal disruption of signaling via the C-terminal domain of "disrupted in schizophrenia 1" (DISC1)-a molecule implicated in psychiatric disorders-resulted in a lack of long-term potentiation (LTP) (persistent strengthening of synapses) and experience-dependent potentiation in adulthood. PMID: 26206934
We demonstrated that Ser704Cys-genotype of DISC1 influences (para)hippocampal structure and functioning the dorsal PFC during executive planning, most prominently in unaffected controls PMID: 25533973
Our results revealed association between rare haplotypes and affective disorder providing further support to the hypothesis that DISC1 sequence variations may be related to AD susceptibility PMID: 24090488
These results suggested a possible role of the DISC1 genotype in the early neurodevelopment of human brains, but failed to show its specific role in the neurodevelopmental pathology of schizophrenia. PMID: 25092219
In Disc1-knockout mice, recombinant human DISC1 regulates the dendritic transport of Itpr1 mRNA by directly interacting with its mRNA. PMID: 25821909
In the adult mammalian brain DISC1 plays a critical role in neurite outgrowth and neuronal migration, particularly in the hippocampus. PMID: 25043320
DISC1 knockdown leads to a reduction of VGF in neurons. PMID: 24934694
These findings highlight the importance of the coordinated expression of DISC1-interactome genes for normal cognitive function. PMID: 24940743
This study provided that DISC1 genetic variants reveals a relationship with the structure and functional connectivity of the precuneus in schizophrenia. PMID: 24909300
Expression of the DB7 fusion gene may reduce protein translation to impair brain functions and thereby contribute to the pathogenesis of major psychiatric disorders. PMID: 24908665
DISC1 forms a complex with dysbindin and increases its stability in association with a reduction in ubiquitylation. PMID: 25635053
The results reinforce the role of DISC1 in the association of schizophrenia PMID: 23855403

Hide All

Involvement in disease

Schizophrenia 9 (SCZD9)

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Mitochondrion. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, synapse, postsynaptic density.

Tissue Specificity

Ubiquitous. Highly expressed in the dentate gyrus of the hippocampus. Also expressed in the temporal and parahippocampal cortices and cells of the white matter.

Database Links

HGNC: 2888

OMIM: 604906

KEGG: hsa:27185

STRING: 9606.ENSP00000355593

UniGene: Hs.13318

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Code	CSB-RA297176A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(20µg), U-251MG whole cell lysate(20µg), HEK293T whole cell lysate(20µg), HL-60 whole cell lysate(20µg), SW620 whole cell lysate(20µg), Rat brain tissue lysate(20µg) All lanes: DISC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 100 kDa Observed band size: 115 kDa Exposure time：1min Overlay Peak curve showing jurkat cells stained with CSB-RA297176A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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DISC1 Recombinant Monoclonal Antibody

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Target Background