Product Details

Uniprot No.

Target Names

Alternative Names

Dihydropyrimidinase-related protein 2 (DRP-2) (Collapsin response mediator protein 2) (CRMP-2) (N2A3) (Unc-33-like phosphoprotein 2) (ULIP-2), DPYSL2, CRMP2 ULIP2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human DPYSL2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20D8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DPYSL2, also known as collapsin response mediator protein 2 (CRMP-2), plays a central role in neuronal development and axonal guidance by mediating semaphorin signaling pathways. This cytosolic phosphoprotein regulates microtubule dynamics and is essential for growth cone collapse and neurite outgrowth. Beyond its developmental functions, DPYSL2 has garnered significant research attention due to its altered expression in neurodegenerative conditions and its emerging roles in cancer biology, making it a valuable target for investigators studying both neurological disorders and tumor progression.

This recombinant monoclonal antibody, clone 20D8, offers the reproducibility and consistency that demanding research applications require. Generated through recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The antibody was raised against a synthetic peptide derived from human DPYSL2 and purified by affinity chromatography, ensuring high specificity for your target.

Validation studies demonstrate reliable performance across multiple experimental platforms. Immunohistochemistry testing in paraffin-embedded human breast cancer tissue reveals clear staining at dilutions between 1:50 and 1:200, providing flexibility to optimize signal intensity for your specific samples. Immunofluorescence and flow cytometry applications have been validated using SH-SY5Y neuroblastoma cells, a particularly relevant model given DPYSL2's neuronal expression pattern. The flow cytometry data shows distinct separation between specific staining and isotype control populations, confirming robust detection of intracellular DPYSL2.

Whether you are investigating axonal regeneration mechanisms, characterizing CRMP-2 phosphorylation states in disease models, or exploring its unexpected roles in cancer tissues, this antibody provides a dependable tool for advancing your neuroscience research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA029079A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA029079A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.35% DAB. Immunofluorescence staining of SH-SY5Y with CSB-RA029079A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 506-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA029079A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

DPYSL2 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background