Product Details

Uniprot No.

Q16555

Target Names

DPYSL2

Alternative Names

DPYSL2 Recombinant Monoclonal Antibody

Species Reactivity

Human

Immunogen

Recombinant Human DPYSL2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1A1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DPYSL2, also known as collapsin response mediator protein 2 (CRMP-2), plays a central role in neuronal development and axon guidance by mediating semaphorin signaling pathways. This cytosolic phosphoprotein regulates microtubule dynamics and is essential for establishing neuronal polarity during brain development. Beyond its neurological functions, DPYSL2 has emerged as a protein of interest in cancer research, where altered expression patterns have been observed across multiple tumor types, making it a valuable marker for investigators studying both neurobiology and oncology.

This recombinant monoclonal antibody, clone 1A1, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced from a stable expression system, you can expect consistent performance across experiments and between lot numbers, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The rabbit host and affinity-purified IgG format ensure high specificity for human DPYSL2 detection.

Validation studies demonstrate reliable performance in immunohistochemistry applications across diverse tissue contexts. Testing on paraffin-embedded human brain tissue confirms detection in DPYSL2's primary site of expression, while additional validation in human colorectal cancer and breast cancer specimens demonstrates utility for researchers investigating this protein's role in malignancy. A working dilution range of 1:50 to 1:200 provides flexibility for optimization across different sample types and detection systems, with validated protocols using citrate buffer antigen retrieval at pH 6.0.

This antibody serves researchers exploring neuronal development, axon regeneration, and the emerging connections between CRMP-2 dysregulation and cancer progression, providing a dependable tool for immunohistochemical analysis of human tissues.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA614975MA1HU
Size	US$210
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Image	IHC image of CSB-RA614975MA1HU diluted at 1:150 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA614975MA1HU diluted at 1:150 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA614975MA1HU diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
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DPYSL2 Recombinant Monoclonal Antibody

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