Code | CSB-RA010418A04me2HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
The production of the di-methyl-Histone H3.1 (K4) recombinant monoclonal antibody is a meticulous process that initiates with the cloning of genes responsible for encoding the HIST1H3A antibody, encompassing both heavy and light chains. These cloned genes are then incorporated into expression vectors, which are subsequently introduced into host cells via transfection. The host cells are then tasked with producing and secreting the antibody. To ensure its purity and efficacy, the antibody undergoes a stringent purification process utilizing affinity chromatography. Following purification, the antibody is rigorously tested for functionality in a range of applications, including ELISA, WB, ICC, and IF, making it a versatile tool for accurately detecting the human and mouse HIST1H3A proteins di-methylated at K4.
Di-methylation of Histone H3.1 at lysine 4 (K4) primarily functions in transcriptional activation, chromatin accessibility, developmental regulation, epigenetic memory, coordinated gene regulation, and has implications in various diseases.
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