EEF2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA053731A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate(30µg), HEK293 whole cell lysate, NIH/3T3 whole cell lysate, HeLa whole cell lysate(30µg) All lanes: eEF2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 95 kDa Observed band size: 115 kDa Exposure time: 10s Immunofluorescence staining of Jurkat cell with CSB-RA053731A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA053731A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P13639

Target Names

EEF2

Alternative Names

EEF 2 antibody; Eef2 antibody; EF-2 antibody; EF2 antibody; EF2_HUMAN antibody; Elongation factor 2 antibody; Eukaryotic translation elongation factor 2 antibody; Polypeptidyl tRNA translocase antibody; SCA26 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human EEF2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Eukaryotic elongation factor 2 (EEF2) serves as an essential component of the protein synthesis machinery, catalyzing the GTP-dependent translocation of the ribosome along messenger RNA during translation. This highly conserved protein plays a critical role in cellular growth and proliferation, making it a significant target in studies of translational control, cancer biology, and cellular stress responses. EEF2 activity is tightly regulated through phosphorylation by EEF2 kinase, positioning it at the intersection of nutrient sensing and protein synthesis pathways.

This recombinant monoclonal antibody, generated from clone 8B4 in rabbit host, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced recombinantly, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis confirms robust detection in both human cell lines, including THP-1, HepG2, Jurkat, HEK293, and HeLa, as well as mouse NIH/3T3 cells, providing valuable cross-species reactivity for comparative studies. The observed band at approximately 115 kDa, higher than the predicted 95 kDa molecular weight, reflects the known post-translational modifications of EEF2, including glycosylation and phosphorylation events. Immunofluorescence staining in Jurkat cells reveals cytoplasmic localization consistent with EEF2's role in ribosomal function, while flow cytometry data demonstrates clear separation from isotype controls.

Whether investigating translational regulation, exploring EEF2's emerging connections to neurodegenerative conditions like spinocerebellar ataxia type 26, or examining metabolic stress responses, this antibody provides reliable detection across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Gene References into Functions

our study indicates that expression of eEF2 protein is a potential biomarker for evaluating prostate cancer PMID: 28913610
eEF2 may be activated in a positive feedback cycle through inactivation of eEF2K via the PI3K/Akt/mTOR pathway. PMID: 30060827
the direct interaction of AKT2 and EF2 was found to be dynamically regulated in embryonic rat cardiomyocytes PMID: 23823123
These results suggest that binding of eEF2 to the ribosome alters its conformation, resulting in a weakened affinity of eIF5A and impairment of this interplay compromises cell growth due to translation elongation defects. PMID: 27115996
nuclear localization of active eEF-2 depends upon its interaction with p53, as cells lacking p53 contain less active eEF-2 in the nuclear compartment PMID: 23542375
High serum eukaryotic elongation factor 2 level is associated with non-small cell lung cancer. PMID: 24377563
Results indicated that the eEF2 gene is overexpressed in the majority of several types of cancers and plays an oncogenic role in cancer cell growth. PMID: 24589652
The expression levels of three putative HA-regulated proteins (TALDO, ANXA1 and EF2) in control, H2O2-, HA- and HA/H2O2-treated OA chondrocytes were verified by Western blotting and the results indeed support the notion that HA acts in anti-oxidation PMID: 24480285
Thiopental inhibits global protein synthesis by repression of eukaryotic elongation factor 2 and protects from hypoxic neuronal cell death. PMID: 24167567
A single amino acid substitution in eukaryotic elongation factor 2 co-segregates with the disease phenotype of spinocerebellar ataxia 26. PMID: 23001565
EEF2 phosphorylation by cyclin A-cyclin-dependent kinase 2 (CDK2) on a novel site, serine 595 (S595), directly regulates T56 phosphorylation by eEF2K. PMID: 23184662
Burn induces prolonged activation of eEF2K and eEF2 in pediatric patients. PMID: 22269896
PHD2 modulated eEF2 activity and protein translation under acute hypoxia. PMID: 22308030
NH125 induces eEF2 phosphorylation (peEF2) through multiple pathways in cancer cells PMID: 22020937
Training in the fasted state, compared with identical training with ample carbohydrate intake, facilitates post-exercise dephosphorylation of eEF2. PMID: 21132439
results suggest that eEF2 is an anti-apoptotic marker in lung adenocarcinoma PMID: 21554491
eEF-2 is activated by both lithium and GSK-3, whereas, lithium treatment and inhibition of GSK-3 have opposing effects on eEF-2. PMID: 20708687
melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
inhibition of protein synthesis under high pressure occurs independent of the phosphorylation of eEF-2 PMID: 15534876
Levels of p-eEF2K were significantly increased, and total eEF2 significantly decreased in Alzheimer disease PMID: 16098202
Data show that overproduction of elongation factor 2 blocks HIV-1 viral protein R-induced cell death both in fission yeast and human cells. PMID: 16520893
Hypoxia inhibits protein synthesis through a 4EBP1 and EEF2 pathway controlled by mTOR and unbcoupled in breast cancer cells. PMID: 16648488
Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type. PMID: 19036825
Overexpressed eEF2 in gastrointestinal cancers promoted G2/M progression and enhanced their cell growth in vitro and in vivo. PMID: 19360331

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Involvement in disease

Spinocerebellar ataxia 26 (SCA26)

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-G/EF-2 subfamily

Database Links

HGNC: 3214

OMIM: 130610

KEGG: hsa:1938

STRING: 9606.ENSP00000307940

UniGene: Hs.515070

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

AMPK signaling Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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