Product Details

Uniprot No.

Target Names

Alternative Names

Eukaryotic initiation factor 4A-I (eIF-4A-I) (eIF4A-I) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-1), EIF4A1, DDX2A EIF4A

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human eIF4A1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EIF4A1, also known as eukaryotic initiation factor 4A-I, serves as a critical ATP-dependent RNA helicase that plays an essential role in translation initiation. By unwinding secondary structures in the 5' untranslated region of mRNAs, this enzyme enables ribosome scanning and efficient protein synthesis. Given its involvement in regulating gene expression at the translational level, EIF4A1 has become an important target for researchers investigating cellular growth, stress responses, and the mechanisms underlying aberrant protein synthesis in disease states.

This recombinant monoclonal antibody, clone 7C3, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit, you can expect uniform performance across lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation testing demonstrates reliable detection across multiple experimental platforms. In western blot applications, the antibody successfully identifies EIF4A1 in K562, HEK293, and RAW264.7 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 50 kDa runs slightly higher than the predicted molecular weight of 40-47 kDa, which likely reflects post-translational modifications such as glycosylation or phosphorylation events common to this protein. Immunofluorescence studies in HeLa cells confirm cytoplasmic localization patterns consistent with EIF4A1's known distribution, with recommended dilutions between 1:20 and 1:200.

Whether you are exploring translational control mechanisms, investigating stress granule dynamics, or examining broader questions in epigenetics and nuclear signaling, this antibody provides a dependable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Gene References into Functions

Star-PAP-specific polyadenylation sites usage regulates the expression of the eukaryotic translation initiation factor EIF4A1, the tumor suppressor gene PTEN and the long non-coding RNA NEAT1. PMID: 28911096
our data demonstrate that the common effects of eIF4A1 and eIF4E on translation are mediated by the coding region and 3'UTR PMID: 27879264
our data identify the eIF4F complex as an important upstream regulator of TORC1, which acts via TSC2 to inactivate TORC1 upon withdrawal of amino acids PMID: 26988032
Our results demonstrate that miR-133a plays a pivotal role in colorectal cancer by inhibiting cell proliferation, invasion, and migration by targeting oncogenic eukaryotic translation initiation factor 4A1, which acts as a tumor suppressor and may provide a new potential therapeutic target in colorectal cancer PMID: 28466778
miR-1284 can function as a new regulator to reduce gastric cancer multidrug resistant cells by targeting EIF4A1. PMID: 26936591
Studies indicate a developing focus on targeting eukaryotic initiation factor 4A eIF4A1 and eIF4A2 as cancer therapy. PMID: 26614665
Immunohistochemical analysis was performed on over 3000 breast tumors to investigate the relationship among expression of eIF4A1, the helicase-modulating proteins eIF4B, eIF4E and PDCD4, and clinical outcome. PMID: 25611378
Collectively, these data suggested that a potential role of NS4A in antagonizing host antiviral defense is by recruiting eIF4AI and escaping the translation inhibition mediated by PKR. PMID: 25866185
eIF4A functions as an adenosine triphosphate-dependent processive helicase when complexed with two accessory proteins, eIF4G and eIF4B. PMID: 26113725
demonstrate that DAP5 associates with eIF2beta and eIF4AI to stimulate IRES-dependent translation of cellular mRNAs PMID: 25779044
Studies indicate that eukaryotic initiation factor 4AI (EIF4AI) undergoes large conformational transitions, while unwinding RNA hairpins. PMID: 24909782
Data indicate that caspase recruitment domain family, member 11 protein CARD11 has complex 5'UTRs and is sensitive to eIF4A RNA helicase inhibition. PMID: 25320244
Increased Expression of EIF4A1 is associated with poor response to Brachytherapy in Cervical Cancer. PMID: 24844222
report of an eIF4A RNA helicase-dependent mechanism of translational control that contributes to oncogenesis and underlies the anticancer effects of silvestrol and related compounds PMID: 25079319
The E186 residue was found to be of significance for RNA-dependent ATPase activity for eIF4AI alone and in the presence of eIF4AI-binding domains of eIF4GI, binding between eIF4AI and eIF4GI domains were also significantly influenced by mutation of E186. PMID: 24471916
Findings implicate phosphorylation of eIF4G1(Ser1232) by Cdk1:cyclin B and its inhibitory effects on eIF4A helicase activity in the mitotic translation initiation shift. PMID: 24248602
Eukaryotic translation initiation factor 4A1 (eIF4A1) is a direct target of miR-US25-2-3p. PMID: 23747307
Regulation of MUC1-C expression is mediated by the PI3K/AKT pathway and the eIF4A RNA helicase. PMID: 22689062
The results indicated that eIF4AI and eIF4AII expression are linked and that the two protein isoforms exhibit functional differences. PMID: 22589333
Duplex unwinding and ATPase activities of the DEAD-box helicase eIF4A are coupled by eIF4G and eIF4B. PMID: 21840318
study found Burkholderia pseudomallei BPSL1549 acted as a potent cytotoxin against eukaryotic cells; it promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation PMID: 22076380
analysis of the tandem MA-3 region of Pdcd4 protein and characterization of its interactions with eIF4A and eIF4G PMID: 21454508
Studies indicate that eIF4A (DDX2), together with its accessory proteins eIF4B and eIF4H, is thought to act as a helicase that unwinds secondary structures in the mRNA 5' UTR. PMID: 21427765
EIF4A and eIF4G proteins are not responsible for the selective translation of viral mRNAs and the translational shut-off of cellular protein synthesis. PMID: 21377182
Inhibition of eIF4A reduced the synthesis of APP and tau. PMID: 20927385
These findings identify PKP1 as a regulator of translation and proliferation via modulation of eIF4A1 activity. PMID: 20156963
Comparative characterization of two DEAD-box RNA helicases in superfamily II: human translation-initiation factor 4A and hepatitis C virus non-structural protein 3 (NS3) helicase. PMID: 11903057
Results indicate that not only binding to eIF4A but also prevention of eIF4A binding to the MA-3 domain of eIF4Gc contributes to the mechanism by which Pdcd4 inhibits translation. PMID: 15082783
analysis of cells of eIF4GIf molecules lacking either the PABP-binding site, the eIF3-binding site, the middle domain eIF4A-binding site, or the C-terminal segment that includes the second eIF4A-binding site PMID: 17130132
Results report that 15d-PGJ2 blocks translation through inactivation of translational initiation factor eIF4A. PMID: 18034160
the PDCD4 MA3 domains compete with the eIF4G MA3 domain and RNA for eIF4A binding. PDCD4 inhibits translation initiation by displacing eIF4G and RNA from eIF4A. PMID: 18296639
The interaction of eIF4AI with two accessory factors, eIF4B and eIF4H, was studied. PMID: 18719248
Study reports the topology of the eIF4A/4G/4H helicase complex, which is built from multiple experimentally observed domain-domain contacts. PMID: 19203580

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Protein Families

DEAD box helicase family, eIF4A subfamily

Database Links

HGNC: 3282

OMIM: 602641

KEGG: hsa:1973

STRING: 9606.ENSP00000293831

UniGene: Hs.129673

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Code	CSB-RA190088A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate All lanes: eIF4A1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 47 kDa Observed band size: 50 kDa Immunofluorescence staining of Hela Cells with CSB-RA190088A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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EIF4A1 Recombinant Monoclonal Antibody

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