Code | CSB-RA190088A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IF | 1:20-1:200 |
The process of producing an EIF4A1 recombinant antibody consists of four main steps. First, the EIF4A1 monoclonal antibody gene is sequenced, followed by cloning it into a plasmid vector. The recombinant vector is then introduced into a host cell line. Once the antibody is expressed by the host cell, it is purified from the cell culture supernatant using affinity chromatography. Finally, the purified antibody is tested and characterized. The EIF4A1 monoclonal antibody is obtained from EIF4A1 antibody-producing hybridomas, and a synthesized peptide from human EIF4A1 is used as the immunogen during its generation. The resulting EIF4A1 recombinant monoclonal antibody is ideal for ELISA, WB, and IF applications in detecting human EIF4A1 protein.
EIF4A1 is an RNA helicase protein that is involved in various aspects of RNA metabolism. Its main function is to unwind the secondary structure of RNA molecules during translation initiation, a crucial step in the process of protein synthesis. EIF4A1 is a component of the eukaryotic translation initiation factor 4F (eIF4F) complex, which also contains other initiation factors such as eIF4E and eIF4G. This complex binds to the 5' cap structure of mRNA and facilitates the recruitment of ribosomes for translation initiation. In addition to its role in translation initiation, EIF4A1 has been implicated in other RNA-related processes, such as RNA splicing and degradation. It also plays a role in cellular stress response pathways and has been shown to be important for cell survival under stress conditions.
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