ELP4 Recombinant Monoclonal Antibody

ELP4 serves as an essential subunit of the Elongator complex, a multifunctional assembly that plays critical roles in transcriptional elongation and tRNA modification. This protein contributes to histone acetylation processes that regulate chromatin accessibility and gene expression, making it a significant target for researchers investigating epigenetic mechanisms and nuclear signaling pathways.

This recombinant monoclonal antibody offers the reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it delivers consistent performance across experiments and eliminates the lot-to-lot variability that can complicate long-term studies. The rabbit host and monoclonal nature ensure high specificity for ELP4 detection across human, mouse, and rat samples, providing valuable cross-species reactivity for comparative studies.

Validation data demonstrates robust performance across multiple applications. Western blot analysis confirms detection of a 47 kDa band matching the predicted molecular weight, with successful results obtained in human cell lines including A431, HEK293, A549, A2780, and Jurkat, as well as mouse and rat brain tissue lysates. This broad sample compatibility supports diverse experimental designs from cell culture models to tissue-based investigations. Immunofluorescence staining in MCF-7 cells reveals clear subcellular localization patterns when counter-stained with DAPI, while flow cytometry analysis in HeLa cells shows distinct positive signal separation from isotype controls.

The antibody's versatility extends to immunohistochemistry applications, offering researchers flexibility to examine ELP4 expression and distribution in fixed tissue sections. For investigators studying transcriptional regulation, tRNA modification pathways, or broader epigenetic mechanisms, this antibody provides a reliable tool for characterizing ELP4 across multiple experimental platforms.