ERLIN2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA215137A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), U251 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: Erlin 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time：120s IHC image of CSB-RA215137A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA215137A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA215137A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

O94905

Target Names

ERLIN2

Alternative Names

C8orf2 antibody; Chromosome 8 open reading frame 2 antibody; Endoplasmic reticulum lipid raft associated protein 2 antibody; Endoplasmic reticulum lipid raft-associated protein 2 antibody; ER lipid raft associated 2 antibody; ERLIN 2 antibody; Erlin-2 antibody; ERLIN2 antibody; ERLN2_HUMAN antibody; HGNC:1356 antibody; MGC87072 antibody; NET32 antibody; SPFH 2 antibody; SPFH domain containing protein 2 antibody; SPFH domain family member 2 antibody; SPFH domain-containing protein 2 antibody; SPFH2 antibody; SPG18 antibody; Stomatin prohibitin flotillin HflC/K domain containing protein 2 antibody; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ERLIN2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

24F7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ERLIN2, also known as endoplasmic reticulum lipid raft-associated protein 2, plays a critical role in ER-associated degradation pathways and cholesterol homeostasis. This protein has garnered significant attention in neuroscience research due to its association with hereditary spastic paraplegia type 18 (SPG18), making it a valuable target for investigators studying neurodegenerative mechanisms and ER stress responses.

This recombinant monoclonal antibody, clone 24F7, offers the reproducibility and consistency that demanding research protocols require. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format, raised against a synthesized peptide from human ERLIN2, has been affinity-purified to ensure high specificity for your target.

Validation data demonstrates robust performance across multiple applications, providing flexibility for diverse experimental workflows. Western blot analysis confirms detection of the expected 38 kDa band across an extensive panel of human cell lines, including HeLa, HepG2, A431, HEK293, PC-3, U251, and THP-1 lysates at 1:1000 dilution. For tissue-based studies, immunohistochemistry has been validated in paraffin-embedded human kidney and liver cancer sections using citrate buffer antigen retrieval at 1:100 dilution. Flow cytometry applications have been confirmed in MCF-7 cells, enabling quantitative analysis of ERLIN2 expression at the single-cell level.

Whether investigating ER membrane organization, lipid metabolism, or the molecular basis of hereditary spastic paraplegia, this antibody provides a reliable tool for advancing your understanding of ERLIN2 biology in human experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1. Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex. Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex.

Gene References into Functions

Novel Mutations in Endoplasmic Reticulum Lipid Raft-associated Protein 2 Gene Cause Pure Hereditary Spastic Paraplegia Type 18 PMID: 27824013
Erlin-2 and the related erlin-1 were found to negatively regulate cholesterol and fatty acid biosynthesis in cultured cell models when the proteins were depleted by RNAi. The proteins also selectively bound cholesterol. PMID: 24217618
ERLIN2 was found to be responsible for causing hereditary spastic paraplegia in a Saudi family. PMID: 23085305
ERLIN2 may confer a selective growth advantage for breast cancer cells by facilitating a cytoprotective response to various cellular stresses associated with oncogenesis. PMID: 22681620
ERLIN2 loss on cell growth may advance understanding of the mechanism behind motor neuron degeneration in primary lateral sclerosis PMID: 23109145
a novel brain gamma-secretase associated protein , erlin-2, that resides in detergent resistant membranes and affects amyloid beta-peptide production. PMID: 22771797
a novel role for ERLIN2 in supporting cancer cell growth by promoting the activation of the key lipogenic regulator SREBP1c and the production of cytosolic lipid droplets. PMID: 22690709
study describes an extended consanguineous Saudi family in which hereditary spastic paraplegia is linked to SPG18, an autosomal recessive locus, and show it is associated with a nullimorphic deletion of ERLIN2 PMID: 21796390
The gene encodes endoplasmic reticulum (ER) lipid raft-associated protein 2 that mediates the ER-associated degradation of activated inositol 1,4,5-trisphosphate receptors and other substrates. PMID: 21330303
SPFH2 as a key endoplasmic reticulum associated degradation pathway component and suggest that it may act as a substrate recognition factor. PMID: 17502376

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Involvement in disease

Spastic paraplegia 18, autosomal recessive (SPG18)

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Note=Associated with lipid raft-like domains of the endoplasmic reticulum membrane.

Protein Families

Band 7/mec-2 family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 1356

OMIM: 611225

KEGG: hsa:11160

STRING: 9606.ENSP00000276461

UniGene: Hs.705490

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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