ERP29 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA909705A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA909705A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA909705A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA909705A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing K562 cells stained with CSB-RA909705A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P30040

Target Names

ERP29

Alternative Names

ERP29 antibody; C12orf8 antibody; ERP28Endoplasmic reticulum resident protein 29 antibody; ERp29 antibody; Endoplasmic reticulum resident protein 28 antibody; ERp28 antibody; Endoplasmic reticulum resident protein 31 antibody; ERp31 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ERP29 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23C9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Endoplasmic reticulum protein 29 (ERP29) serves as a critical molecular chaperone within the secretory pathway, facilitating the proper folding and processing of nascent proteins destined for secretion or membrane insertion. This protein plays essential roles in ER stress responses and has garnered significant attention in cancer research, where altered expression patterns have been linked to tumor progression and therapeutic resistance mechanisms.

This recombinant monoclonal antibody, clone 23C9, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically to the last, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host origin combined with monoclonal specificity provides excellent signal-to-noise characteristics across multiple detection platforms.

Validation studies demonstrate robust performance in immunohistochemistry, where the antibody successfully detects ERP29 in both normal human liver tissue and liver cancer specimens at 1:100 dilution using citrate buffer antigen retrieval. This dual-tissue validation is particularly valuable for researchers investigating ERP29 expression changes in hepatic malignancies. Immunofluorescence applications have been confirmed in HeLa cells, revealing characteristic ER localization patterns when counterstained with DAPI. Flow cytometry validation in K562 cells demonstrates clear population separation from isotype controls, supporting intracellular staining protocols for suspension cell analysis.

The antibody arrives affinity-purified and ready for use across ELISA, immunohistochemistry, immunofluorescence, and flow cytometry workflows. For investigators exploring ER stress pathways, protein secretion mechanisms, or the emerging connections between ERP29 and cancer biology, this antibody provides a dependable tool for advancing signal transduction research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Gene References into Functions

Downregulation of ERp29 was commonly found in GC tissues and highly correlated with more aggressive phenotypes and poorer prognosis. PMID: 28874138
The anti-aggregation peptide ReACp53 significantly decreased ERP29 expression and suppressed the chemoresistant effect. These findings highlight a role of ERP29 in the acquired chemoresistance of cancer cells expressing the aggregating p53 mutant Arg282Trp. PMID: 28534505
Results of further analyses by using a CNX mutant imply that ERp29 and ERp57 recognize the same domain of CNX, whereas the mode of interaction with CNX might be somewhat different between them. PMID: 28456374
High ERP29 expression is associated with pancreatic ductal adenocarcinomas. PMID: 26887611
our studies prove a novel function of ERp29\MGMT in cancer cell survival against radiation. Targeting ERp29\MGMT axis may be useful for providing better treatment efficacy in combination with radiotherapy in breast cancer. PMID: 26420420
Endoplasmic reticulum protein 29 attenuates CSE-induced ER stress and enhances cell viability and barrier integrity of RPE cells, and therefore may act as a protective mechanism for RPE survival and activity. PMID: 26431474
CLIC4, ERp29, and Smac/DIABLO integrated into a novel panel based on cancer stem-like cells in association with metastasis stratify the prognostic risks of colorectal cancer. PMID: 24916695
overexpression of ERp29 may play a key role in apoptosis in HTR-8/SVneo cells via activation of p38, which may participate in the pathogenesis of intrahepatic cholestasis of pregnancy PMID: 24780196
ERp29 directs ENaC toward the Golgi, where it undergoes cleavage during its biogenesis and trafficking to the apical membrane. PMID: 24944201
Apoptosis related proteins ERp29, PRDX6 and MPO were differentially expressed in placentas of pregnant women with intrahepatic cholestasis and in healthy pregnant women. PMID: 24391750
S1P1 overexpression or ERp29 absence is related to the carcinogenesis and progression, and may be potential biomarkers for early detection of gallbladder adenocarcinoma. PMID: 23558074
ERp29 is a novel molecule that regulates MET and epithelial cell integrity in breast cancer cells. PMID: 22543584
Overexpression of ERp29 increases the radioresistance in nasopharyngeal carcinoma. PMID: 21479953
Suggest that ERp29 associates with radioresistance in nasopharyngeal carcinoma and may be potential biomarker for predicting response to radiotherapy. PMID: 22160175
Eurycomanone affects the transcription of Erp28, annexin 1 and prohibitin resulting in reduced expression of these proteins. PMID: 21903368
interplay between p38 phosphorylation and p58(IPK) upregulation has key roles in modulating ERp29-induced cell-growth arrest and survival PMID: 22064321
ERp29 is a 4PBA-regulated ER chaperone that regulates WT-CFTR biogenesis and can promote DeltaF508-CFTR trafficking in CF epithelial cells PMID: 21525008
Results identify ERp29 as a novel regulator of PERK and provide evidence for the role of ER resident factors in the regulation of chemotherapeutic efficacy. PMID: 21419175
High expression of ERp29 inversely correlates to tumor progression. PMID: 20920593
Expression and tissue distribution of ERp29 [review] PMID: 12362325
ERp29 is an unusual redox-inactive member of the thioredoxin family [review] PMID: 16677078
ERP29 is expressed in a subset of basal-cell carcinoma of the skin, with the infiltrating carcinomas exhibiting the highest incidence of immunopositivity. PMID: 17012915
Results found that ERp29 comprises significant alpha-helical structure. PMID: 17073718
mutants of the D-domain of ERp29 prevent transport of a substrate protein (Pipe) in a manner consistent with the presence of a discrete, conserved peptide binding site in the D-domain PMID: 17296603
ERp29-activated polyomavirus perforates the physiologically relevant ER membrane, an event that likely initiates viral penetration. PMID: 17881435
lactating mammary glands were expressing ERp29 while the resting glands did not PMID: 18395818
there are two putative peptide binding sites of ERp29 and different processing activity of the human and Drosophila proteins in vivo does not stem from differences in peptide binding properties. PMID: 19084538
The level of ER protein 29 (ERp29) was shown to be decreased in the CK19-expressing BT549 breast cancer cell line. PMID: 19265690
This supports a new role for ERp29 as a chaperone that helps stabilize monomeric Cx43 to enable oligomerization to occur in the Golgi apparatus. PMID: 19321666
ERp29 is a novel regulator leading to cell growth arrest and cell transition from a proliferative to a quiescent state. PMID: 19770839

Hide All

Subcellular Location

Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity

Ubiquitous. Mostly expressed in secretory tissues.

Database Links

HGNC: 13799

OMIM: 602287

KEGG: hsa:10961

STRING: 9606.ENSP00000261735

UniGene: Hs.75841

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address