Product Details

Uniprot No.

Target Names

FTO

Alternative Names

AlkB homolog 9 antibody; ALKBH9 antibody; Alpha-ketoglutarate-dependent dioxygenase FTO antibody; AW743446 antibody; Fat mass and obesity-associated protein antibody; FATSO; MOUSE; HOMOLOG OF antibody; Fto antibody; FTO_HUMAN antibody; GDFD antibody; KIAA1752 antibody; mKIAA1752 antibody; Protein fatso antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human FTO

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4G9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

FTO, the fat mass and obesity-associated protein, functions as an alpha-ketoglutarate-dependent dioxygenase with a pivotal role in RNA demethylation, particularly the removal of N6-methyladenosine modifications from mRNA. This enzymatic activity positions FTO at the intersection of epitranscriptomic regulation and metabolic signaling, making it a compelling target for researchers investigating obesity, metabolic disorders, and increasingly, neurological processes where RNA modifications influence gene expression dynamics.

This recombinant monoclonal antibody, clone 4G9, offers the reproducibility that demanding research applications require. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, you can expect consistent performance across experiments and between lot numbers, eliminating a common source of variability in long-term studies.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Western blot analysis confirms specific detection of the 59 kDa FTO protein across a diverse panel of human cell lines including 293T, SH-SY5Y, Raji, Colo320, A549, and PC3, providing confidence when working with various cellular models. The observed band size aligns with the predicted molecular weight, indicating clean target recognition. For tissue-based studies, immunohistochemistry has been validated in paraffin-embedded human glioma cancer and skeletal muscle tissue using citrate buffer antigen retrieval, while immunofluorescence staining in HeLa cells reveals the expected nuclear localization pattern characteristic of this demethylase.

Whether you are exploring FTO's emerging roles in neuroscience research or investigating its established connections to metabolic regulation, this antibody provides a reliable tool for protein detection and localization studies across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression.

Gene References into Functions

Bi-directional relationship between BMI and affective symptoms across the life span, modified by the FTO rs9939609 polymorphism. PMID: 29531329
We showed that MC4R rs17782313 and FTO rs9939609 were positively associated with zBMI, with weak and very weak effects, respectively, suggesting a very scarce contribution to childhood obesity. PMID: 29679223
the minor allele A of the rs9939609 has a significant association with increasing BMI values in the Emirati population PMID: 29343214
There is an association of FTO rs9939609 A/A genotype and impaired fasting glucose and insulin resistance. Homozygous A genotype diabetic patients may be more vulnerable to blood glucose fluctuation. PMID: 30273662
Our results suggested that genetic variants in the FTO gene were strongly associated with BMI in Chinese women PMID: 29657248
rs8050136 A/C and rs1588413 C/T associated with polycystic ovary syndrome susceptibility PMID: 29463151
Mechanistically, FTO enhanced MZF1 expression by reducing m(6)A levels and mRNA stability in MZF1 mRNA transcript, leading to oncogenic functions. PMID: 29842885
Nineteen single nucleotide polymorphisms in the FTO gene were tested. Allelic analysis showed that allele T of SNP rs1121980 was a risk allele. Our study suggests that rs1121980 can become a biomarker for the screening and prognosis of Intervertebral Disc Degeneration PMID: 30099472
FTO rs9939609 variant may not be associated with insulin resistance in Indonesian obese female adolescents. PMID: 29764479
FTO polymorphisms appear to be universally associated with the risk of obesity, and further investigation into this genetic locus may provide clues for potential therapeutic targets PMID: 29466028
The polymorphism of FTO gene rs17817449 and GNB3 gene rs5443 (C825T) may be a genetic determinant of obesity in Saudi population whereas impact of MC4R Asn274Ser change could not be detected. PMID: 29937877
A lack of association between FTO polymorphismsand GDM risk. PMID: 30055308
Our findings demonstrate a relationship between C allele carriers on the FTO gene and a predisposition to a higher fat mass and body fat percentage. PMID: 30124167
FTO variant is not associated with osteoarthritis in the Chinese Han population. PMID: 29606151
This study confirms an association between the FTO gene and adiposity markers in Chilean adults, which is independent of major confounding factors. PMID: 30148903
FTO gene polymorphisms are associated with variability of HDL-cholesterol concentrations which may lead to increased CVD risk in patients with acromegaly who are risk-allele carriers. PMID: 28913579
We observed no evidence for associations of rs1421085 in FTO with FatOx and RQ. This indicates that the rs1421085-C allele in FTO induces obesity likely via other pathways than via reduced FatOx. PMID: 28626215
This study reports interactions between the FTO variant and each of: frequency of alcohol consumption; deviations from mean sleep duration; overall diet, including added salt; and physical activity. PMID: 27596730
FTO rs9939609 Polymorphism is associated with Obesity. PMID: 28566238
Study showed that a haplotype in the first intron of the FTO gene had a strong association with obesity indices among Iranian adolescent males. None of the students with GGC genotypes were underweight, while most of the students with AAT had high body mass. PMID: 29677190
The rs9939609 polymorphism in the FTO gene may be a genetic risk factor for malignant pleural mesothelioma. PMID: 29260910
FTO was highly expressed in motor neurons and is associated with with sporadic amyotrophic lateral sclerosis in Greek patients. PMID: 29216901
A longer duration of exclusive breastfeeding (EXBF) has substantial impact on body mass index (BMI) growth trajectories among children carrying the FTO adverse variant by modulating the age at adiposity peak, age at adiposity rebound and BMI velocities. EXBF acts antagonistically to the FTO rs9939609 risk allele and by the age of 15. PMID: 29040503
FTO rs9939609 is associated with obesity risk and LTL in this study, where this association is only observed at higher, but not lower, FTO methylation levels of participants. Our data suggest association of multiple factors, including FTO methylation level, may be involved in one of several mechanisms underlying the commonly reported obesity risk of this FTO polymorphism. PMID: 28559540
This study indicates that FTO expression may have an important role in promoting the occurrence of gastric cancer (GC), and it may be an vital molecular marker in the diagnosis and prognosis of GC patients PMID: 28849183
Mediterranean Diet adherence can be useful for prevention or treatment of obesity phenotypes in subjects with FTO risk alleles. PMID: 28954439
Two FTO variants, found in 14 affected individuals from three families, were also associated with leanness in these patients with Delayed Puberty. One variant (p.Leu44Val) demonstrated altered demethylation activity of the mutant protein in vitro. Mutations in genes implicated in body mass and timing of puberty in the general population may contribute to the pathogenesis of self-limited delayed puberty. PMID: 29161441
rs9939609 may be a potential biomarker in early diagnosis or gene therapy target of endometrial cancer and pancreatic cancer. [Meta-Analysis] PMID: 26931363
The aggregation analysis revealed a higher correlation between siblings than between parent-offspring pairs representing the role of genetic factors in metabolic syndrome (MetS). In addition, the conditional logistic model with covariates showed that the linkage results between HDL_C and three markers, FTO (rs1558902 and rs7202116) and CETP(rs1864163) were significant. PMID: 29548861
genetic association studies in population of adolescents in the United States: Data suggest that an SNP in FTO (rs9939609) is associated with adolescent overweight/obesity and obesogenic appetitive traits (decreased satiety responsiveness and increased food responsiveness) in the population studied. PMID: 28712975
This meta-analysis provides additional support for a significant interaction between FTO, depression and BMI, indicating that depression increases the effect of FTO on BMI. PMID: 28642257
children at risk for obesity possessing the obesity risk polymorphism (FTO rs9939609) exhibited stronger responses to food commercials in the nucleus accumbens (NAcc) than children not at risk. Similarly, children at a higher genetic risk for obesity possessing the obesity risk polymorphism (FTO rs9939609) demonstrated larger NAcc volumes. PMID: 27994159
Variations within FTO may be predictors of fatty liver disease in HIV-infected patients independently of metabolic factors. PMID: 28116842
Studied weight loss in obese patients with Perilipin 4 (PLIN4), Fat mass and obesity-associated (FTO), and beta- adrenergic receptor 3 (ADRB3) polymorphisms treated with Garcinia cambogia/Glucomannan. Results suggest weight loss was attenuated in carriers of PLIN4, FTO, ADRB3 polymorphisms. PMID: 29361938
Fat mass and obesity associated (FTO) was the first gene found to be associated with obesity in three independent genome-wide association studies. PMID: 27324062
It has been shown, that presence of one mutant allele of rs9939609 (gene FTO) and rs4994 (gene ADRB3) leads to statistically significant association with obesity. PMID: 29381017
Food advertisement exposure was associated with greater caloric consumption of a recently advertised food, and this effect was modified by an FTO genotype. Future research is needed to understand the neurological mechanism underlying these associations. PMID: 27654143
FTO rs9939609 polymorphism is observed to be associated with obesity. PMID: 29317321
FTO rs9939609 polymorphism in recurrent VTE may differ according to gender. PMID: 29325734
the first intron of the FTO gene is associated with obesity in humans in a mechanism potentially involving the risk allele (rs1421085) PMID: 27544196
The age of diabetes was not affect by the tested FTO polymorphisms (rs9939609 , rs1421085, and rs9930506). PMID: 28585683
Infants carrying the GG genotype of the LEP rs7799039 polymorphism were 2.12 times more likely to be born large for gestational age (LGA) than those carrying the GA + AA genotypes. Newborns carrying the TG or GG genotype of the ADIPOQ rs2241766 polymorphism were 1.88 times more likely to be born LGA than those carrying the TT genotype. No association was found between the FTO gene polymorphism and newborn weight status. PMID: 27392994
FTO obesity risk allele is associated with differential neural processing of food images. PMID: 26797854
Data confirm the association between the FTO first intron polymorphism and the presence of type 2 diabetes mellitus in the Slavonic (Czech) population. The same variant is likely to be associated with development of chronic complications of diabetes mellitus, especially with diabetic neuropathy and diabetic kidney disease in either T2DM or both T1DM and T2DM. PMID: 29154870
This study demonstrated that the rs9939609 (FTO) polymorphism showed a relationship with obesity in the population studied and an interaction with cardiorespiratory fitness (CRF). Students with low levels of CRF and the AA genotype have a higher risk of being overweight/obese. PMID: 26458076
Data show that the splicing effects of alpha-ketoglutarate dependent dioxygenase FTO are dependent on the catalytic activity in vivo and are mediated by N6-methyladenosine (m6A). PMID: 28977517
FTO variants not only were associated with T2DM, but also some variants had a strong association with apelin and androgenic hormones profile. PMID: 29101069
FTO and near MC4R variants are associated with obesity measures in Sri Lankan populations... PMID: 26948330
Results suggest that the hypomorphic FTO p.A134T variant is associated with thiopurine-induced leukopenia in South Korean patients with inflammatory bowel disease. PMID: 27558924
The FTO gene polymorphism, rs9939609, was found to be associated with insulin resistance, insulin, triglyceride and adiponectin levels in obese patients with TT variant PMID: 27759977

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Involvement in disease

Growth retardation, developmental delay, and facial dysmorphism (GDFD); Obesity (OBESITY)

Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

Fto family

Tissue Specificity

Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. Highly expressed in highly expressed in acute myeloid leukemias (AML) with t(11;11)(q23;23) with KMT2A/MLL1 rearrangements, t(15

Database Links

HGNC: 24678

OMIM: 601665

KEGG: hsa:79068

STRING: 9606.ENSP00000418823

UniGene: Hs.528833

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Code	CSB-RA880154A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate All lanes: FTO antibody at 0.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 15, 7, 13 KDa Observed band size: 59 KDa IHC image of CSB-RA880154A0HU diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA880154A0HU diluted at 1:70 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-RA880154A0HU at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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FTO Recombinant Monoclonal Antibody

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