Product Details

Uniprot No.

Target Names

Alternative Names

GBP 1 antibody; GBP-1 antibody; GBP1 antibody; GBP1_HUMAN antibody; GTP binding protein 1 antibody; GTP-binding protein 1 antibody; Guanine nucleotide binding protein 1 antibody; Guanine nucleotide-binding protein 1 antibody; Guanylate binding protein 1 antibody; Guanylate binding protein 1 interferon inducible 67kDa antibody; Guanylate binding protein 1 interferon inducible antibody; HuGBP 1 antibody; HuGBP-1 antibody; HuGBP1 antibody; Interferon induced guanylate binding protein 1 antibody; Interferon-induced guanylate-binding protein 1 antibody; OTTHUMP00000012352 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human GBP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12E12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Guanylate-binding protein 1 (GBP1) serves as a critical effector in interferon-mediated immune responses, playing essential roles in host defense against intracellular pathogens and contributing to inflammatory signaling pathways. As an interferon-inducible GTPase, GBP1 has emerged as an important marker in immunology research, with growing interest in its involvement in tumor microenvironment modulation and cellular stress responses.

This recombinant rabbit monoclonal antibody, clone 12E12, offers the consistency and reliability that demanding experimental workflows require. Generated through recombinant technology, this antibody provides sequence-defined specificity against human GBP1, ensuring reproducible results across experiments and eliminating the lot-to-lot variability that can complicate long-term studies. The affinity-purified IgG format delivers clean signal with minimal background interference.

Validation data demonstrates robust performance across multiple applications. Western blot analysis confirms specific detection of GBP1 at the expected 68 kDa molecular weight across diverse human cell lines, including A431 epidermoid carcinoma, Jurkat T lymphocytes, U251 glioblastoma, and 786-O renal carcinoma cells, providing confidence when working with various cellular models. Immunohistochemistry staining has been successfully performed on paraffin-embedded human thyroid tissue and ovarian cancer specimens, revealing clear target localization suitable for tissue-based investigations. Flow cytometry validation using 786-O cells demonstrates effective intracellular detection with distinct population separation from isotype controls.

Whether investigating interferon signaling mechanisms, characterizing immune cell populations, or examining GBP1 expression patterns in disease contexts, this antibody provides a versatile tool for immunology research. The validated performance across western blotting, immunohistochemistry, flow cytometry, and ELISA applications supports integration into diverse experimental strategies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA986451A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:A431 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U251 whole cell lysate(30µg), 786-O whole cell lysate(30µg) All lanes: GBP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time：120s IHC image of CSB-RA986451A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA986451A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA986451A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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GBP1 Recombinant Monoclonal Antibody

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