GCSAM Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA440526A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:Raji whole cell lysate(30µg) All lanes: GCSAM antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time：120s IHC image of CSB-RA440526A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Raji cells stained with CSB-RA440526A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q8N6F7

Target Names

GCSAM

Alternative Names

GCAT2 antibody; Gcet antibody; GCET2 germinal center expressed transcript 2 antibody; GCET2 germinal centre expressed transcript 2 antibody; Gcsam antibody; GCSAM_HUMAN antibody; Germinal center associated lymphoma antibody; Germinal center associated lymphoma protein antibody; Germinal center B cell associated protein 2 antibody; Germinal center B-cell-expressed transcript 2 protein antibody; Germinal center expressed transcript 2 antibody; Germinal center-associated lymphoma protein antibody; Germinal center-associated signaling and motility protein antibody; Germinal centre associated lymphoma antibody; Germinal centre associated lymphoma protein antibody; Germinal centre B cell associated protein 2 antibody; Germinal centre expressed transcript 2 antibody; hGAL antibody; M17 antibody; M17 L antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human GCSAM protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

GCSAM (Germinal Center-Associated Signaling and Motility protein), also known as GCET2, plays a specialized role in germinal center B-cell biology, where it contributes to the signaling and motility processes essential for effective humoral immune responses. This protein serves as a valuable marker for studying germinal center dynamics, B-cell differentiation, and lymphoma biology, making it particularly relevant for immunology research focused on adaptive immunity and B-cell malignancies.

This recombinant monoclonal antibody, clone 25B7, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology in rabbit host, it provides sequence-defined specificity against human GCSAM, ensuring reproducible results across experiments and eliminating the lot-to-lot variability that can complicate long-term studies.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis of Raji whole cell lysate reveals a clean band at the predicted 21 kDa molecular weight, confirming accurate target recognition in this germinal center-derived B-cell lymphoma line. Immunohistochemistry testing in paraffin-embedded human gastric cancer tissue shows effective performance in FFPE workflows, while flow cytometry analysis of Raji cells demonstrates clear positive signal separation from isotype controls, supporting intracellular staining applications.

The antibody arrives in a stabilized liquid format with glycerol for convenient long-term storage, and its unconjugated state allows flexibility in secondary antibody selection for your detection system of choice. Whether you are investigating germinal center biology, characterizing B-cell populations, or exploring GCSAM expression patterns in tissue samples, this antibody provides a dependable tool for advancing your immunology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.

Gene References into Functions

Diagnostic Utility of the Germinal Center-associated Markers GCET1, HGAL, and LMO2 in Hematolymphoid Neoplasms. PMID: 25203428
These findings further elucidate the growing and complex role of HGAL in B-cell biology and suggest that membrane-bound and cytoplasmic HGAL protein differently regulates distinct biological processes. PMID: 25381061
HGAL and LMO2 may be useful adjuncts in the identification of follicular lymphoma of the nongastric gastrointestinal tract. PMID: 22914613
the germinal centre protein human germinal centre-associated lymphoma regulates B-cell receptor signalling in B-lymphocytes which, without appropriate control, may lead to B-cell lymphoproliferation. PMID: 23299888
HGAL/GCET2 protein expression may function as a marker for germinal center B-cell type diffuse large B-cell lymphoma. PMID: 22743653
Immunostaining for HGAL was more frequently positive than that for BCL6 and CD10 in follicular lymphomas PMID: 21988858
Data suggest that both HGAL and LMO2 are directly regulated by the transcription repressor PRDM1--overexpression of PRDM1 down-regulates HGAL and LMO2; PRDM1 directly binds to promoters of both HGAL and LMO2 and represses genetic transcription. PMID: 21722313
necessary for germinal center maintenance and confining B-cells to the germinal center microenvironment PMID: 21475040
show that HGAL increases the binding of myosin to F-actin and inhibits the ability of myosin to translocate actin by reducing the maximal velocity of myosin head/actin movement PMID: 21447067
HGAL is sensitive and specific marker for detecting follicular lymphoma in nodal and extranodal sites PMID: 21502424
molecular mechanism underlying the inhibitory effects of germinal center-specific HGAL protein on the motility of GC-derived lymphoma cells PMID: 20844236
Our results show that HGAL and LMO2 are sensitive markers for follicular lymphoma diagnosis. PMID: 20697248
Study confirms and validates findings of a correlation between HGAL expression and outcome in classical HL. PMID: 19883310
gene is induced by IL4 and expression strongly predicts survival in diffuse large B-cell lymphoma PMID: 12509382
Results report the cloning of germinal center B-cell expressed transcripts 1 and 2 (GCET1 and 2), and show that both are expressed in follicular lymphoma and diffuse large B-cell lymphoma with germinal center B-cell differentiation PMID: 12819018
The restricted expression and germinal center specificity of HGAL protein suggest that it may have an important role in the diagnosis of specific lymphomas PMID: 15677569
HGAL is involved in negative regulation of lymphocyte migration, thus constraining lymphocytes to the germinal center PMID: 17823310
The correlation of HGAL expression with the germinal center B-cell phenotype demonstrates the role of this marker in the classification of cutaneous large B-cell lymphomas PMID: 18264083
results report the cloning of HGAL, a germinal center its expression pattern in normal tissues and hematologic malignancies, its induction by IL4, and the prognostic significance of its expression in predicting survival in diffuse large B-cell lymphoma PMID: 12509382

Hide All

Subcellular Location

Cytoplasm. Cell membrane. Note=It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.

Tissue Specificity

Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen.

Database Links

HGNC: 20253

OMIM: 607792

KEGG: hsa:257144

STRING: 9606.ENSP00000419485

UniGene: Hs.49614

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address