GORASP1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA223163A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg) All lanes: GORASP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 65 kDa Exposure time: 3min IHC image of CSB-RA223163A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A431 cells stained with CSB-RA223163A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9BQQ3

Target Names

GORASP1

Alternative Names

FLJ23443 antibody; Golgi peripheral membrane protein p65 antibody; Golgi phosphoprotein 5 antibody; Golgi reassembly and stacking protein 1 antibody; Golgi reassembly and stacking protein 65 kDa antibody; Golgi reassembly and stacking protein, 65-kD antibody; Golgi reassembly stacking protein 1 65kDa antibody; Golgi reassembly stacking protein 1 antibody; Golgi reassembly stacking protein of 65 kDa antibody; Golgi reassembly-stacking protein 1 antibody; Golgi reassembly-stacking protein of 65 kDa antibody; GOLPH 5 antibody; GOLPH5 antibody; Gorasp 1 antibody; GORASP1 antibody; GORS1_HUMAN antibody; GRASP 65 antibody; GRASP65 antibody; MGC118894 antibody; MGC118897 antibody; P65 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human GORASP1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

GORASP1, also known as GRASP65, serves as a critical structural component of the Golgi apparatus, where it plays essential roles in maintaining cisternal stacking and facilitating Golgi reassembly following mitotic division. This peripheral membrane protein participates in the intricate regulation of secretory pathway trafficking and has emerged as a significant target in studies examining Golgi dynamics, cell cycle progression, and membrane organization.

This recombinant monoclonal antibody, clone 11D3, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology in rabbit host, it provides sequence-defined specificity against human GORASP1, ensuring that results remain comparable across extended studies and between laboratory groups. The affinity-chromatography purification yields a reagent suitable for quantitative applications where background interference must be minimized.

Validation data demonstrates reliable performance across multiple experimental platforms. In western blot analysis of HEK293 whole cell lysate, the antibody detects a band at approximately 65 kDa at dilutions of 1:500–1:2000. While the predicted molecular weight is 46 kDa, the observed higher mass likely reflects post-translational modifications, particularly phosphorylation events characteristic of this Golgi phosphoprotein. Immunohistochemistry studies in paraffin-embedded human small intestine tissue confirm utility for examining GORASP1 distribution in fixed specimens, while flow cytometry analysis using A431 cells demonstrates clear positive signal separation from isotype controls, supporting intracellular staining applications.

This antibody serves researchers investigating Golgi architecture, membrane trafficking mechanisms, and cellular stress responses affecting organelle integrity. Its validated performance across complementary techniques provides flexibility for studies requiring orthogonal confirmation of GORASP1 expression and localization patterns.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane.

Gene References into Functions

In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells. PMID: 28782625
Results demonstrate a critical role for GRASP55 and GRASP65 in maintaining the stacked structure of the Golgi, which is required for accurate posttranslational modifications in the Golgi. Additionally, the GRASP knockout cell lines developed in this study will be useful tools for studying the role of GRASP proteins in other important cellular processes. PMID: 28814501
The authors determined that Golgi membrane ribbon fragmentation increased during the early cytoplasmic phase of cytomegalovirus virion assembly and that Golgi membrane fragmentation in infected cells was dependent on the phosphorylation of an integral cis-Golgi protein, Grasp65. PMID: 27703074
In cells, Mena and actin were required for Golgi ribbon formation after nocodazole washout; in vitro, Mena and microfilaments enhanced GRASP65 oligomerization and Golgi membrane fusion. Thus Mena interacts with GRASP65 to promote local actin polymerization, which facilitates Golgi ribbon linking. PMID: 26538023
Mutagenesis experiments support these structural observations and demonstrate that they are required for GRASP65-GM130 association. PMID: 26363069
Cisternal-specific functions of GRASP65 and GRASP55 in continuity, compartmentalization, and function of the Golgi ribbon. PMID: 24227884
propose that GRASP55/65 are negative regulators of exocytic transport and that this slowdown helps to ensure more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi network PMID: 23552074
The C-terminal fragments of GRASP65 produced following caspase cleavage are targeted to mitochondria, and ectopic expression of these sensitises HeLa cells to Fas ligand. PMID: 21368855
the mechanism of phosphoinhibition as direct inhibition by PLK1 of the PDZ ligand underlying the GRASP65 self-interaction. PMID: 20937827
GRASP65 has a role in Golgi cisternal stacking and cell cycle progression PMID: 20214750
These results demonstrate that GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism. PMID: 20083603
Data demonstrate that both GRASP55 and 65 are needed for the efficient transport to and through the Golgi complex, thus highlighting a novel role for the GRASPs in membrane trafficking. PMID: 19840934
the GRASP domain alone of GRASP65 inhibits mitotic fragmentation of the Golgi apparatus PMID: 15576368
GRASP65 may function as a signal integrator controlling the cell growth PMID: 15834132
GRASP65 has a role in the regulation of spindle dynamics rather than a direct role in the stacking of Golgi cisternae PMID: 15888544
Ribbon formation requires the Golgi proteins GM130 and GRASP65. PMID: 16489344
Mitochondria bearing GRASP65 became tethered to one another, and this depended on a GRASP65 PDZ domain that was also required for GRASP65 self-interaction. PMID: 19581411
A study showing that GRASP65 binds directly to the coiled-coil vesicle tethering factor GM130, and targets it to Golgi membranes. PMID: 9628863
Identification of GRASP65, and demonstration that it functions in the formation of stacked Golgi cisternae. PMID: 9346242

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Subcellular Location

Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum-Golgi intermediate compartment membrane.

Protein Families

GORASP family

Database Links

HGNC: 16769

OMIM: 606867

KEGG: hsa:64689

STRING: 9606.ENSP00000313869

UniGene: Hs.721946

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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