GPA33 Recombinant Monoclonal Antibody

Code CSB-RA245537A0HU
Size US$210
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Image
  • IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Target Names
GPA33
Alternative Names
Cell surface A33 antigen (Glycoprotein A33), GPA33
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human GPA33
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
4H4
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

GPA33 has high homology with CD2 and possesses a V-type Ig-like domain at its N terminus. It's also linked to T cell activation inhibitory proteins including CD276 (B7-H3) and VSIG4, as well as many cell adhesion molecules like CEACAM, ICAM, and NCAM (3D-Protein BLAST, NCBI). Immune dysfunction has been linked to GPA33. Polymorphisms in the gene encoding GPA33 have been linked to the autoimmune condition eosinophilic granulomatosis with polyangiitis. Furthermore, the GPA33 gene is found in a Sjögren's Syndrome susceptibility locus. Hypersensitivity to dietary allergens and increased inflammatory responses to experimentally caused colitis was seen in a GPA33 knockout mice model.

The preparation of the recombinant GPA33 antibody involves the mammalian cell lines expression of plasma vectors containing GPA33 antibody genes. B cells isolated from immunized animals' blood were treated to obtain RNA, which underwent reverse transcription to yield DNA genes. Antibody genes were sequenced and screened from the DNA. After transient expression, cell supernatant was collected and then purified using Affinity-chromatography to obtain the recombinant GPA33 antibody. This recombinant GPA33 antibody is recommended to use in the GPA33 for the detection of GPA33 protein from Human.

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Target Background

Function
May play a role in cell-cell recognition and signaling.
Gene References into Functions
  1. A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID: 28226180
  2. A33 expression is directly correlated to colorectal tumor differentiation. PMID: 27272411
  3. Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID: 23230278
  4. Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID: 23294608
  5. The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID: 22623777
  6. Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID: 22623782
  7. cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID: 12114523
  8. Regulation of A33 antigen expression by GKLF. PMID: 12853980
  9. This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID: 18236042

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Database Links

HGNC: 4445

OMIM: 602171

KEGG: hsa:10223

STRING: 9606.ENSP00000356842

UniGene: Hs.651244

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