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GPA33 Antibody

Datasheet
Code CSB-RA245537A0HU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No. Q99795
Target Names GPA33
Alternative Names Cell surface A33 antigen (Glycoprotein A33), GPA33
Species Reactivity Human
Immunogen A synthesized peptide derived from human GPA33
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 4H4
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function May play a role in cell-cell recognition and signaling.
Gene References into Functions
  1. A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID: 28226180
  2. A33 expression is directly correlated to colorectal tumor differentiation. PMID: 27272411
  3. Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID: 23230278
  4. Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID: 23294608
  5. The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID: 22623777
  6. Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID: 22623782
  7. cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID: 12114523
  8. Regulation of A33 antigen expression by GKLF. PMID: 12853980
  9. This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID: 18236042

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Database Links

HGNC: 4445

OMIM: 602171

KEGG: hsa:10223

STRING: 9606.ENSP00000356842

UniGene: Hs.651244
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