GPA33 Antibody

Datasheet

Code	CSB-PA857459EA01HU
Size	US$166
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Image	Immunofluorescence staining of MCF-7 cells with CSB-PA857459EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: GPA33 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 50 kDa Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA857459EA01HU at dilution of 1:100
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GPA33 Polyclonal antibody

Uniprot No.

Q99795

Target Names

GPA33

Alternative Names

A33 antibody; Cell surface A33 antigen antibody; Cell surface A33 antigen precursor antibody; Glycoprotein A33 (transmembrane) antibody; Glycoprotein A33 antibody; Gpa33 antibody; GPA33_HUMAN antibody; MGC129986 antibody; MGC129987 antibody; OTTHUMP00000032346 antibody; Transmembrane glycoprotein A33 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cell surface A33 antigen protein (22-235AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GPA33 Antibody (Product code: CSB-PA857459EA01HU) is Non-conjugated. For GPA33 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA857459EB01HU	GPA33 Antibody, HRP conjugated	ELISA
FITC	CSB-PA857459EC01HU	GPA33 Antibody, FITC conjugated
Biotin	CSB-PA857459ED01HU	GPA33 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May play a role in cell-cell recognition and signaling.

Gene References into Functions

A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID: 28226180
A33 expression is directly correlated to colorectal tumor differentiation. PMID: 27272411
Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID: 23230278
Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID: 23294608
The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID: 22623777
Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID: 22623782
cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID: 12114523
Regulation of A33 antigen expression by GKLF. PMID: 12853980
This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID: 18236042

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.

Database Links

HGNC: 4445

OMIM: 602171

KEGG: hsa:10223

STRING: 9606.ENSP00000356842

UniGene: Hs.651244

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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