GPA33 Antibody

Datasheet

Code	CSB-PA857459EA01HU
Size	US$166
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Image	Immunofluorescence staining of MCF-7 cells with CSB-PA857459EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: GPA33 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 50 kDa Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA857459EA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GPA33 Polyclonal antibody

Uniprot No.

Q99795

Target Names

GPA33

Alternative Names

A33 antibody; Cell surface A33 antigen antibody; Cell surface A33 antigen precursor antibody; Glycoprotein A33 (transmembrane) antibody; Glycoprotein A33 antibody; Gpa33 antibody; GPA33_HUMAN antibody; MGC129986 antibody; MGC129987 antibody; OTTHUMP00000032346 antibody; Transmembrane glycoprotein A33 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cell surface A33 antigen protein (22-235AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GPA33 Antibody (Product code: CSB-PA857459EA01HU) is Non-conjugated. For GPA33 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA857459EB01HU	GPA33 Antibody, HRP conjugated	ELISA
FITC	CSB-PA857459EC01HU	GPA33 Antibody, FITC conjugated
Biotin	CSB-PA857459ED01HU	GPA33 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May play a role in cell-cell recognition and signaling.

Gene References into Functions

A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID: 28226180
A33 expression is directly correlated to colorectal tumor differentiation. PMID: 27272411
Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID: 23230278
Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID: 23294608
The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID: 22623777
Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID: 22623782
cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID: 12114523
Regulation of A33 antigen expression by GKLF. PMID: 12853980
This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID: 18236042

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.

Database Links

HGNC: 4445

OMIM: 602171

KEGG: hsa:10223

STRING: 9606.ENSP00000356842

UniGene: Hs.651244

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