GUCY2C Monoclonal Antibody

Code CSB-RA010053A2HU
Size US$350
Image
  • The Binding Activity of Huamn GUCY2C with Anti-GUCY2C recombinant Antibody.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C (CSB-MP010053HUd9) at 5 μg/mL can bind Anti-GUCY2C recombinant antibody, the EC50 is 3.049-4.660 ng/mL.
  • T84 cells were stained with Mouse IgG2a (green line) and anti-GUCY2C antibody (red line) (2µg/1*106cells), washed and then followed by FITC-conjugated Goat Anti-Mouse IgG(H+L) antibody and analyzed with flow cytometry.
  • Untransfected CT26 cells (green line) and transfected Human GUCY2C CT26 stable cells (red line) were stained with anti-GUCY2C antibody (2µg/1*106cells), washed and then followed by FITC-conjugated Goat Anti-Mouse IgG(H+L) antibody and analyzed with flow cytometry.
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Product Details

Uniprot No. P25092
Target Names GUCY2C
Alternative Names GC-C antibody; Guanylyl cyclase C antibody; GUC2C antibody; GUC2C_HUMAN antibody; GUCY2C antibody; Heat stable enterotoxin receptor antibody; Heat-stable enterotoxin receptor antibody; hSTAR antibody; Intestinal guanylate cyclase antibody; STA receptor antibody
Species Reactivity Mouse
Immunogen Recombinant Human GUCY2C protein
Immunogen Species Mouse
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2a
Clone No. 5F9
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, FC
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Protocols ELISA Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.
Gene References into Functions
  1. High Expressions of guanylyl cyclase C is associated with rectal cancer. PMID: 28418917
  2. The findings support that the activating mutation in GUCY2C creates an intestinal environment with a major influence on the microbiota, which could contribute to the increased susceptibility to inflammatory bowel disease in patients with Familial GUCY2C diarrhea syndrome. PMID: 28902124
  3. the expression of GCC is maintained throughout the process of tumor progression and formation of metastatic disease. PMID: 29261789
  4. To investigate gut motility and hormones before and after a meal in Familial GUCY2C diarrhea syndrome patients and compare with healthy controls PMID: 28957388
  5. Mutations in GUCY2C indicate a role for this receptor in the pathogenesis of sporadic Congenital secretory diarrhea. PMID: 25994218
  6. these data support Ad5-GUCY2C-PADRE as a safe and effective vaccination strategy in preclinical models and position Ad5-GUCY2C-PADRE for Phase I clinical testing in colorectal cancer patients. PMID: 27903079
  7. Familial GUCY2C diarrhoea syndrome is caused by an activating mutation in the GUCY2C gene, which causes impaired contractility and fluid stagnation in the small bowel PMID: 27338166
  8. Findings show how caloric suppression of the guanylin-GUCY2C signaling axis links obesity to negation of a universal tumor suppressor pathway in colorectal cancer. PMID: 26773096
  9. Data show that individuals affected with meconium ileus (MI) had either homozygous or compound heterozygous variants in enterotoxin receptor GUCY2C. PMID: 25370039
  10. High guanylyl cyclase C expression is associated with colon cancer recurrence. PMID: 24919572
  11. Transmission disequilibrium tests (TDT), case-control studies and quantitative analyses indicated association between GUC2C with Attention-deficit/hyperactivitydisorder and its core symptoms. PMID: 25064385
  12. Intestinal cell proliferation and senescence are regulated by receptor guanylyl cyclase C and p21. PMID: 24217248
  13. GUCY2C signaling constitutes a direct link between the initiation of colorectal cancer and the induction of its associated desmoplastic stromal niche. PMID: 24085786
  14. Data show that lower survival (OS) and disease-free survival (DFS) rates were significantly associated with guanylate cyclase C (GCC) and CK20 mRNA levels. PMID: 23150200
  15. Interaction with VIP36 is dependent on glycosylation at the same sites that allow GC-C to fold and bind ligand. PMID: 23269669
  16. GUCY2C opposes systemic genotoxic tumorigenesis by regulating AKT-dependent intestinal barrier integrity PMID: 22384056
  17. Mutations in GUCY2C were associated with the Meconium ileus phenotype. downregulation of GUCY2C leads to a cystic fibrosis-like intestinal phenotype. PMID: 22521417
  18. We identified a heterozygous missense mutation (c.2519G-->T) in GUCY2C in all family members with familial diarrhea syndrome. PMID: 22436048
  19. High guanylyl cyclase C is associated with colon cancer. PMID: 21533822
  20. Data suggest that monitoring the peripheral blood GCC expressions may allow employing different treatment options to metastatic CRC patients. PMID: 21901559
  21. Lymph nodes (range: 2-159) from 291 prospectively enrolled node-negative colorectal cancer patients were analyzed by histopathology and GUCY2C quantitative RT-PCR. PMID: 21307149
  22. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
  23. A novel PDZ protein regulates the activity of guanylyl cyclase C, the heat-stable enterotoxin receptor. PMID: 11950846
  24. guanylyl cyclase c is ectopically expressed in primary and metastatis adenocarcinoma of the esophagus and stomach PMID: 12163327
  25. SH3 domain -mediated protein-protein interaction with the catalytic domain of guanylyl cyclase C inhibited the cyclase activity and might be pivotal for the desensitization phenomenon of the guanylyl cyclase C receptor PMID: 12649275
  26. gelatinolytic activity of GCC in colorectal tumor tissues may facilitate the hepatic metastatic process in the steps after intravasation but not during or before intravasation PMID: 14581363
  27. a novel role for glycosidic modification of GC-C during its biosynthesis, in imparting subtle conformational changes in the receptor that allow for ligand-mediated activation and perhaps regulation of basal activity PMID: 14748740
  28. Lack of guanylyl cyclase c expression assoeciated with significant reduction in adenomas in Apc(Min/+);Gucy2c(-/-) mice PMID: 15825168
  29. Transformation associated with reflux at the gastroesophageal junction reflects activation by bile acid and acid of a transcriptional program involving NF-kappaB and Cdx2, which mediate intestinal metaplasia and ectopic expression of GC-C. PMID: 16618413
  30. This validated assay is being applied to approximately 10,000 lymph nodes in a prospective trial to define the sensitivity of Guanylyl cyclase C(GCC) qRT-PCR for staging patients with colorectal cancer. PMID: 16899600
  31. docking of the c-src SH2 domain to phosphorylated GC-C results in colocalization and further activation of c-src. PMID: 19620276
  32. the role of the linker region in receptor guanylyl cyclases PMID: 19648115
  33. GCC is a specific marker for differentiating primary and secondary metatstatic ovarian mucinous neoplasms. PMID: 19694825
  34. Results suggest that GUCY2C quantitative reverse transcriptase (qRT)-PCR can be used to detect occult lymph node metastases in pN0 colorectal cancer patients. PMID: 19895223
  35. Expression of the receptor guanylyl cyclase C and its ligands in reproductive tissues of the rat: a potential role for a novel signaling pathway in the epididymis. PMID: 12444076
  36. The protein kinase-like domain in receptor guanylyl cyclase C provides a critical structural link between the extracellular domain and the catalytic domain in regulation of this family of receptors. PMID: 11478887

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Involvement in disease Diarrhea 6 (DIAR6); Meconium ileus (MECIL)
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.
Protein Families Adenylyl cyclase class-4/guanylyl cyclase family
Database Links

HGNC: 4688

OMIM: 601330

KEGG: hsa:2984

STRING: 9606.ENSP00000261170

UniGene: Hs.524278

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