Code | CSB-RA034362A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
FC | 1:50-1:200 |
The synthesis of the H2AFX recombinant monoclonal antibody entails a meticulously planned process involving recombinant DNA and in vitro cloning. The H2AFX antibody genes are cloned into expression vectors. Subsequently, these vectors are introduced into host cells, creating a conducive environment for the recombinant antibody's expression within a cell culture milieu. After expression, the antibody is subjected to affinity chromatography purification. Through rigorous testing, this antibody can be used in ELISA, IHC, and FC applications to detect human histone H2AX.
Histone H2AX and its phosphorylated form, γ-H2AX, are critical components of the cellular response to DNA damage. They serve as markers of DNA damage, enabling the recruitment of repair proteins to damaged sites and contributing to the maintenance of genomic integrity and cell viability.
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