HAPLN1 Recombinant Monoclonal Antibody

HAPLN1, also known as cartilage link protein 1, plays a fundamental role in extracellular matrix organization by stabilizing the interaction between hyaluronan and proteoglycans such as aggrecan and versican. This stabilization is essential for maintaining the structural integrity of cartilage and other connective tissues, making HAPLN1 a protein of significant interest in research areas spanning cartilage biology, tissue development, and signal transduction pathways that govern matrix remodeling.

This recombinant rabbit monoclonal antibody, clone 8F4, offers the consistency and reproducibility that demanding experimental workflows require. Because it is generated from a defined sequence rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity, ensuring that results remain comparable across extended studies and collaborative projects. The antibody was raised against a synthetic peptide derived from human HAPLN1 and demonstrates cross-species reactivity with human, mouse, and rat samples, providing flexibility for comparative studies and translational research models.

Validation across multiple platforms confirms the antibody's versatility. Western blot analysis detects HAPLN1 in diverse human cell lines including COLO205, MCF7, U87, SH-SY5Y, and A2780, as well as in mouse and rat brain tissue lysates. The observed band at approximately 48 kDa, slightly higher than the predicted 40 kDa molecular weight, likely reflects post-translational modifications such as glycosylation, which is characteristic of this secreted extracellular matrix protein. Immunohistochemistry validation in human melanoma tissue and flow cytometry analysis in HepG2 cells further demonstrate reliable detection in both fixed tissue and cell-based applications.

Whether investigating matrix biology, cartilage pathology, or signal transduction mechanisms, this antibody provides a dependable tool for characterizing HAPLN1 expression and localization.