Code | CSB-RA256295A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The production of the HIF1AN recombinant monoclonal antibody involves a precise and standardized process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal using the synthesized peptide derived from human HIF1AN as the immunogen. Total RNA is then extracted from these B cells and converted into cDNA through reverse transcription. The HIF1AN antibody genes are amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is subsequently introduced into host cells to facilitate the production of the HIF1AN recombinant monoclonal antibody. After harvesting the antibody from the cell culture supernatant, it undergoes purification using affinity chromatography to obtain a highly purified form. Rigorous characterization assays, including ELISA, WB, IHC, IF, and FC analysis, are conducted to confirm the antibody's specificity and functionality, ensuring its accurate recognition of human HIF1AN protein. Through this meticulous production process, a dependable and effective HIF1AN recombinant monoclonal antibody is generated, well-suited for diverse applications in HIF1AN-related research.
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