Code | CSB-RA998378A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
The production of the HMBS recombinant monoclonal antibody involves a meticulous and standardized process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal, utilizing a synthesized peptide derived from human HMBS as the immunogen. Subsequently, total RNA is extracted from the isolated B cells and converted into cDNA through reverse transcription. The HMBS antibody genes are amplified using PCR with specific primers targeting the antibody constant regions and then inserted into an expression vector. This vector is introduced into host cells, allowing for the production of the HMBS recombinant monoclonal antibody. The antibody is collected from the cell culture supernatant and subjected to affinity chromatography for purification, resulting in a highly purified formulation. Rigorous characterization assays, including ELISA and WB analysis, are performed to confirm the antibody's specificity and functionality, ensuring its precise recognition of human HMBS protein.
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