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A recombinant monoclonal antibody targeting HNF4A was generated through a series of steps. Initially, a rabbit was immunized with a synthesized peptide derived from human HNF4A protein. B cells were then isolated from the immunized rabbit, and RNA was extracted from these cells. The RNA was reverse-transcribed into cDNA, which was utilized as a template to extend HNF4A antibody genes using degenerate primers. These engineered HNF4A antibody genes were incorporated into a plasmid vector and introduced into host cells for expression. The resultant HNF4A recombinant monoclonal antibody was subsequently purified from the cell culture supernatant via affinity chromatography and evaluated for its performance in ELISA, WB, IHC, IF, and FC applications, demonstrating specific recognition of human and rat HNF4A protein.
The HNF4A protein is a critical transcription factor that regulates gene expression in various tissues, with a primary focus on the liver. Its functions are essential for maintaining metabolic homeostasis, organ development, and the proper functioning of multiple physiological processes, including glucose and lipid metabolism, detoxification, and pancreatic function.
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