Product Details

Uniprot No.

Target Names

Alternative Names

Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) (TCF-14) (Transcription factor HNF-4), HNF4A, HNF4 NR2A1 TCF14

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from Human HNF4A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10A7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Hepatocyte nuclear factor 4-alpha (HNF4A) serves as a master regulator of liver-specific gene expression and plays essential roles in hepatocyte differentiation, glucose metabolism, and lipid homeostasis. As a member of the nuclear receptor superfamily, this transcription factor has emerged as a critical focus in research spanning metabolic disorders, hepatocellular carcinoma, and developmental biology, making reliable detection tools indispensable for advancing these fields.

This recombinant monoclonal antibody offers the consistency and reproducibility that demanding research requires. Generated through recombinant technology with a defined clone (10A7), it provides sequence-level certainty and eliminates the lot-to-lot variability that can compromise longitudinal studies. The rabbit IgG format, raised against a synthetic peptide derived from human HNF4A, delivers high specificity for your target across multiple experimental platforms.

Validation data demonstrates robust performance across diverse applications. Western blot analysis confirms detection of the expected 52 kDa band in both HL-60 whole cell lysate and rat liver tissue, supporting the antibody's cross-species reactivity with human and rat samples. Immunohistochemistry staining in paraffin-embedded human gastric cancer tissue reveals clear nuclear localization patterns, while immunofluorescence studies in MCF-7 cells provide excellent visualization of HNF4A distribution when counter-stained with DAPI. Flow cytometry analysis in HepG2 cells shows distinct positive population shifts compared to isotype controls, confirming utility for quantitative single-cell applications.

Whether investigating nuclear receptor signaling pathways, exploring metabolic regulation in hepatic models, or examining HNF4A dysregulation in cancer contexts, this antibody delivers the flexibility and reliability needed to move your research forward with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA571090A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HL-60 whole cell lysate, Rat Liver tissue lysate, All lanes: HNF-4-alpha antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa IHC image of CSB-RA571090A0HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB. Immunofluorescence staining of MCF-7 with CSB-RA571090A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA571090A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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HNF4A Recombinant Monoclonal Antibody

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