|Have Questions?||Leave a Message or Start an on-line Chat|
In the quest to produce the histone H3.1 recombinant monoclonal antibody, the initial phase involves the extraction of genes encoding the HIST1H3A antibody from rabbits that have been previously exposed to a synthesized peptide derived from the human HIST1H3A protein. These HIST1H3A antibody genes are then seamlessly integrated into specialized expression vectors. Following this genetic modification, the modified vectors are introduced into host suspension cells, which are carefully cultured to stimulate the expression and secretion of antibodies. Subsequently, the Histone H3.1 recombinant monoclonal antibody is subjected to a meticulous purification process utilizing affinity chromatography techniques, effectively isolating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through a diverse range of assays, including ELISA, WB, IHC, IF, and FC tests, unequivocally confirming its ability to interact effectively with the human histone H3.1.
Histone H3.1, a variant of the histone H3 protein family, along with other histone variants and post-translational modifications, plays a fundamental role in shaping the epigenetic landscape of the genome, influencing gene expression, and maintaining genomic integrity. Its dynamic interactions with DNA and various proteins are critical for the proper functioning of the cell.
There are currently no reviews for this product.
Hi, we study DNA and chromatin replication and are intrigued by the IF staining pattern of your “H3.1” antibody (CSB-RA010418A0HU). We have 3 questions.
Q1: Could you explain why the pattern is heterogeneous among single cells?
Q2: Does it only recognize newly incorporated histone H3.1?
Q3: I believe it is a bit of a strange pattern for H3.1 but it might recognize a modified version of the histone.
A1: Heterogenicity usually exists in DNA, RNA, and all other levels of proteins, while Histone H3.1 plays the key role in transcriptional control, DNA repair, DNA replication, and chromosome stabilization, which owns the Heterogenicity among single cells as well. The CSB-RA010418A0HU antibody is specific for Histone H3.1, so the IF pattern of CSB-RA010418A0HU also shows the Heterogenicity among single cells.
A2: The antibody CSB-RA010418A0HU can not only recognize newly incorporated Histone H3.1 but also specifically recognize all Histone H3.1 in the samples.
A3: Theoretically speaking, no matter whether the Histone H3.1 is modified or not, as long as the binding site to CSB-RA010418A0HU has not been changed, then it can be recognized by CSB-RA010418A0HU.