Human IgG1&Igκ Isotype Recombinant Monoclonal Antibody

Isotype controls serve as essential negative controls in flow cytometry and immunostaining experiments, enabling researchers to distinguish true antigen-specific signals from background fluorescence caused by non-specific antibody binding. This Human IgG1 and Igκ Isotype Recombinant Monoclonal Antibody provides the critical baseline reference needed to validate experimental results and ensure accurate interpretation of data when using human IgG1 primary antibodies.

Developed using recombinant technology with clone 4D2, this isotype control offers the consistency and reproducibility that rigorous experimental design demands. Unlike traditional hybridoma-derived antibodies, recombinant production ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody undergoes affinity chromatography purification, achieving greater than 95% purity as confirmed by SEC-HPLC analysis.

This isotype control has been validated for flow cytometry applications at recommended dilutions of 1:50 to 1:200. Experimental testing demonstrates its utility as a proper negative control alongside TSPAN8-targeting antibodies, with validation performed using both untransfected and transfected MC38 stable cells, as well as HT29 cells. In these experiments, the isotype control established clear baseline fluorescence levels when detected with FITC-conjugated anti-Human IgG Fc secondary antibody, allowing for confident discrimination of specific TSPAN8 staining.

Supplied in a liquid format containing 50% glycerol and PBS at pH 7.4 with Proclin 300 preservative, this antibody maintains stability when stored at -20°C or -80°C. Researchers working in immunology and stem cell biology will find this isotype control indispensable for generating publication-quality flow cytometry data with properly controlled experimental conditions.