IL11RA Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA184907A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), Hela whole cell lysate(30µg), HT-29 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: IL 11 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 45 kDa Observed band size: 55 kDa Exposure time：20s IHC image of CSB-RA184907A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

Q14626

Target Names

IL11RA

Alternative Names

Interleukin-11 receptor subunit alpha (IL-11 receptor subunit alpha) (IL-11R subunit alpha) (IL-11R-alpha) (IL-11RA), IL11RA

Species Reactivity

Human

Immunogen

A synthesized peptide from human IL11RA protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Interleukin-11 receptor subunit alpha (IL11RA) serves as the ligand-binding component of the IL-11 receptor complex, playing a central role in cytokine signaling pathways that regulate hematopoiesis, bone metabolism, and inflammatory responses. IL-11 signaling through this receptor has garnered significant research attention for its involvement in fibrotic diseases, cancer progression, and stem cell biology, making reliable detection of IL11RA essential for investigators studying these processes.

This recombinant monoclonal antibody, clone 6B2, offers the consistency and reproducibility that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived antibodies. This reliability is particularly valuable for longitudinal studies or when standardizing protocols across laboratory teams.

Validation testing demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects IL11RA in a range of human cell lines including Jurkat, HeLa, HT-29, SH-SY5Y, and THP-1 whole cell lysates at dilutions between 1:500 and 1:2000. The observed band at approximately 55 kDa runs slightly higher than the predicted 45 kDa molecular weight, a shift consistent with post-translational glycosylation typical of membrane-associated receptor proteins. Immunohistochemistry validation in paraffin-embedded human prostate cancer tissue confirms suitability for tissue-based studies, with effective staining achieved at 1:50 to 1:200 dilutions following citrate buffer antigen retrieval.

This antibody supports researchers investigating IL-11 signaling in oncology, fibrosis, and inflammatory disease contexts, providing a dependable tool for characterizing receptor expression across cell culture and tissue specimens.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Receptor for interleukin-11 (IL11). The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells (Probable). Essential for the normal development of craniofacial bones and teeth. Restricts suture fusion and tooth number.; Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling.; Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling.

Gene References into Functions

The results presented here reveal an additional function in classic IL-11 signaling, highlighting the importance of the IL-11R stalk in IL-11 signaling PMID: 29523682
The D2 domain of the IL-11R contains two N-linked glycans, which are dispensable for its biological activity, but differentially control maturation and intracellular trafficking of the receptor. PMID: 29533934
High IL11RA expression is associated with endometrioid tumours. PMID: 28186993
Report IL11RA and MELK amplification in gastric cancer cell lines and primary gastric adenocarcinomas. PMID: 27920471
These results indicate that IL-11Ralpha is a potential target for the development of molecular targeted therapy and noninvasive tumor imaging in human osteosarcoma. PMID: 25524575
IL11RA promoter polymorphism--rs1061758--may be associated with the risk of papillary thyroid cancer in the Korean population. PMID: 21982075
data suggest that IL-11Ralpha-CAR T cells may represent a new therapy for patients with osteosarcoma pulmonary metastases PMID: 22075555
IL11 as well as IL11RA are likely to play a role in the progression of endometrial carcinoma. PMID: 20553623
IL-11Ralpha was expressed in both epithelial and stromal cells, with epithelial staining being more intense PMID: 12200462
the interleukin-11 receptor alpha-chain has evidence of antiapoptotic effects in human colonic epithelial cells PMID: 14701802
IL-11Ralpha is a candidate target for translational clinical trials against advanced and metastatic prostate cancer. PMID: 14744752
interleukin-11 receptor is expressed in CD38-positive cells from patients with multiple myeloma PMID: 15512823
High expression of interleukin-11 receptor alpha is associated with Hodgkin's lymphoma PMID: 16291580
High interleukin 11 receptor is associated with breast cancer PMID: 16614887

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Involvement in disease

Craniosynostosis and dental anomalies (CRSDA)

Subcellular Location

[Interleukin-11 receptor subunit alpha]: Membrane; Single-pass type I membrane protein.; [Soluble interleukin-11 receptor subunit alpha]: Secreted.; [Isoform HCR2]: Secreted.

Protein Families

Type I cytokine receptor family, Type 3 subfamily

Tissue Specificity

Expressed in a number of cell lines, including the myelogenous leukemia cell line K-562, the megakaryocytic leukemia cell line M-07e, the erythroleukemia cell line TF-1, and the osteosarcoma cell lines, MG-63 and SaOS-2. Also expressed in normal and malig

Database Links

HGNC: 5967

OMIM: 600939

KEGG: hsa:3590

STRING: 9606.ENSP00000326500

UniGene: Hs.591088

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Jak-STAT signaling pathway Hematopoietic cell lineage Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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