INMT Recombinant Monoclonal Antibody

Indolethylamine N-methyltransferase (INMT) catalyzes the methylation of tryptamine and related compounds, playing a central role in the biosynthesis of endogenous trace amines including N,N-dimethyltryptamine. This enzyme's involvement in both metabolic pathways and signal transduction mechanisms has made it an increasingly important target for researchers investigating neuroactive compound metabolism, endogenous psychedelic biosynthesis, and broader methyltransferase biology.

This recombinant monoclonal antibody, clone 31G7, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity against the human INMT peptide immunogen, while affinity chromatography purification delivers a clean preparation suitable for sensitive detection methods.

Validation studies demonstrate robust performance across complementary applications. Immunohistochemistry on paraffin-embedded human lung tissue using a Leica Bond system with citrate buffer antigen retrieval shows clear target detection at dilutions between 1:50 and 1:200. Flow cytometry analysis using the SH-SY5Y neuroblastoma cell line, a model frequently employed in neuroscience research, confirms intracellular INMT detection with distinct signal separation from isotype controls when cells are fixed and permeabilized. This dual-platform validation provides researchers flexibility in experimental design, whether examining tissue architecture or quantifying expression at the single-cell level.

The unconjugated format allows pairing with your preferred secondary detection system, accommodating diverse instrumentation and multiplexing strategies. For investigators exploring methyltransferase function, trace amine metabolism, or related signaling pathways, this antibody provides a sequence-defined, thoroughly validated tool for advancing your research.