INMT Antibody

Datasheet

Code	CSB-PA011723LA01HU
Size	US$166
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Image	Western blot All lanes: INMT antibody at 2µg/ml + Mouse lung tissue Secondary Goat polyclonal to rabbit lgG at 1/15000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA011723LA01HU at dilution of 1:100 Immunofluorescence staining of A549 cells with CSB-PA011723LA01HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INMT Polyclonal antibody

Uniprot No.

O95050

Target Names

INMT

Alternative Names

Amine N methyltransferase antibody; Amine N-methyltransferase antibody; Aromatic alkylamine N methyltransferase antibody; Aromatic alkylamine N-methyltransferase antibody; Arylamine N methyltransferase antibody; Arylamine N-methyltransferase antibody; Indolamine N-methyltransferase antibody; Indolethylamine N methyltransferase antibody; Indolethylamine N-methyltransferase antibody; Inmt antibody; INMT_HUMAN antibody; nicotine N methyltransferase antibody; TEMT antibody; thioester S methyltransferase like antibody; Thioether S methyltransferase antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Indolethylamine N-methyltransferase protein (1-263AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The INMT Antibody (Product code: CSB-PA011723LA01HU) is Non-conjugated. For INMT Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA011723LB01HU	INMT Antibody, HRP conjugated	ELISA
FITC	CSB-PA011723LC01HU	INMT Antibody, FITC conjugated
Biotin	CSB-PA011723LD01HU	INMT Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds. Catalyzes the N-methylation of tryptamine and structurally related compounds.

Gene References into Functions

study suggests that rs77743549 of INMT may be associated with the risk for Hirschsprung's disease PMID: 26183064
genome-wide association studies in populations in Argentina/Bangladeshi: Three SNPs in INMT (rs6970396, rs1061644, rs4270015) are associated with production of trimethylselenonium (TMSe); urinary Se excretion is up-regulated in TMSe-producing women. PMID: 26537946

Subcellular Location

Cytoplasm.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family

Tissue Specificity

Widely expressed. The highest levels were in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in adult a

Database Links

HGNC: 6069

OMIM: 604854

KEGG: hsa:11185

UniGene: Hs.632629

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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