Product Details

Uniprot No.

Target Names

Alternative Names

Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18), KRT18, CYK18

Species Reactivity

Human

Immunogen

Recombinant Human KRT18 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

5F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Keratin 18 is a type I intermediate filament protein that pairs with keratin 8 to form the primary cytoskeletal network in simple epithelial cells. This structural protein serves as a valuable marker in cancer research, particularly for identifying carcinomas of epithelial origin, and its cleavage products have emerged as important indicators of apoptosis in various disease contexts. The dynamic regulation of KRT18 expression and its post-translational modifications make it a compelling target for studies investigating epithelial differentiation, cellular stress responses, and tumor biology.

This recombinant monoclonal antibody against human KRT18 offers the reproducibility essential for longitudinal studies and multi-site collaborations. Produced using recombinant technology with a defined sequence, the antibody eliminates the lot-to-lot variability that can compromise experimental consistency. The human IgG1 isotype format, combined with affinity chromatography purification, ensures high specificity for your target while minimizing background interference.

Validation studies demonstrate reliable performance across multiple experimental platforms. Immunofluorescence staining in both MCF-7 breast cancer cells and HepG2 hepatocellular carcinoma cells reveals the characteristic cytoplasmic filamentous network expected for this intermediate filament protein, with recommended dilutions ranging from 1:50 to 1:200. Flow cytometry analysis in HT29 colorectal adenocarcinoma cells confirms robust detection with clear separation from isotype control, supporting quantitative applications in cell population studies.

This antibody serves researchers investigating epithelial biology, cancer diagnostics, apoptosis mechanisms, and cytoskeletal organization. Its validated performance in immunofluorescence and flow cytometry provides flexibility for both imaging-based localization studies and high-throughput cellular analysis across diverse epithelial cancer models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA012519MA1HU
Size	$49.9
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Image	Immunofluorescence staining of MCF-7 cell with CSB-RA012519MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L). Immunofluorescence staining of HepG2 cell with CSB-RA012519MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L). Overlay Peak curve showing HT29 cells stained with CSB-RA012519MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KRT18 Recombinant Monoclonal Antibody

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