Product Details

Uniprot No.

Target Names

Alternative Names

Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18), KRT18, CYK18

Species Reactivity

Human

Immunogen

A synthesized peptide from human KRT18 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Keratin 18 is a type I intermediate filament protein that pairs with keratin 8 to form the primary cytoskeletal network in simple epithelial cells. This structural protein serves as a valuable marker for epithelial differentiation and has gained significant attention in cancer research, where its expression patterns and cleavage products provide insights into tumor biology and apoptotic processes. KRT18 detection is particularly relevant for studies involving carcinomas, liver pathology, and epithelial-to-mesenchymal transition.

This recombinant monoclonal antibody, clone 7G12, offers the consistency and reproducibility that demanding research applications require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human KRT18, making it a reliable tool for quantitative and qualitative analyses.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Western blot analysis confirms detection of the expected 48 kDa band across diverse human cell lines including HepG2, COLO-205, MCF-7, Jurkat, A549, HeLa, and HEK293, providing confidence when working with various epithelial and non-epithelial models. Immunohistochemistry validation in human lung cancer and small intestine tissues shows clear cytoplasmic staining patterns appropriate for tissue-based studies. Additional confirmation through immunofluorescence in HepG2 cells and flow cytometry analysis of HT29 cells expands the range of applications available for your research.

Whether investigating epithelial marker expression in tumor samples, characterizing cell line identity, or studying cytoskeletal dynamics, this antibody provides a dependable foundation for KRT18 detection across immunoassay, imaging, and cell analysis platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA796043A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), A549 whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293 whole cell lysate(30µg) All lanes: Cytokeratin 18 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time：40s IHC image of CSB-RA796043A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA796043A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA796043A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HT29 cells stained with CSB-RA796043A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KRT18 Recombinant Monoclonal Antibody

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