Product Details

Uniprot No.

P08727

Target Names

KRT19

Alternative Names

Keratin, type I cytoskeletal 19 (Cytokeratin-19) (CK-19) (Keratin-19) (K19), KRT19

Species Reactivity

Human

Immunogen

A synthesized peptide from human KRT19 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9F12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Keratin 19 serves as a critical intermediate filament protein in simple epithelia and has become one of the most widely utilized markers in cancer research and clinical diagnostics. As the smallest member of the keratin family, KRT19 plays essential roles in maintaining cellular structural integrity and is frequently overexpressed in various carcinomas, making it an invaluable target for studying epithelial-derived malignancies and metastatic disease.

This recombinant monoclonal antibody, generated from clone 9F12, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Western blot analysis confirms robust detection in a diverse panel of human cell lines including MCF-7, A549, HT-29, HepG2, HeLa, and PC-3, with the observed band appearing at approximately 49 kDa rather than the predicted 44 kDa—a shift commonly attributed to post-translational modifications such as glycosylation. Immunohistochemistry staining has been validated in human breast cancer and stomach tissue sections, revealing clear cytoplasmic localization patterns. The antibody also performs reliably in immunofluorescence and flow cytometry applications using MCF-7 cells, providing flexibility for both fixed-cell imaging and quantitative single-cell analysis.

Whether you're investigating epithelial differentiation, characterizing tumor samples, or developing diagnostic assays, this KRT19 antibody delivers the specificity and multi-platform compatibility needed for rigorous cancer biology and pathology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA598178A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg) All lanes: Cytokeratin 19 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 44 kDa Observed band size: 49 kDa Exposure time：10s IHC image of CSB-RA598178A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA598178A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with CSB-RA598178A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA598178A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KRT19 Recombinant Monoclonal Antibody

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