LMNB2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA266964A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HeLa whole cell lysate(30µg), THP-1 whole cell lysate, PC-3 whole cell lysate(30µg) All lanes: LMNB2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70 kDa Observed band size: 68 kDa Exposure time: 3min IHC image of CSB-RA266964A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA266964A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Jurkat cell with CSB-RA266964A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA266964A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q03252

Target Names

LMNB2

Alternative Names

LAMB 2 antibody; LAMB2 antibody; Lamin-B2 antibody; LMN 2 antibody; LMN B2 antibody; LMN2 antibody; LMNB 2 antibody; LMNB2 antibody; LMNB2_HUMAN antibody; MGC2721 antibody; RGD1563803 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human LMNB2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15C5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Lamin B2 is a key component of the nuclear lamina, the fibrous meshwork underlying the inner nuclear membrane that provides structural support to the nucleus and plays essential roles in chromatin organization, DNA replication, and cell cycle regulation. As a B-type lamin, LMNB2 is expressed constitutively across cell types and has emerged as an important marker in studies of nuclear architecture, cellular senescence, and various malignancies where nuclear envelope integrity is compromised.

This recombinant monoclonal antibody against human LMNB2 offers the reproducibility and consistency that demanding research applications require. Generated through recombinant technology using clone 15C5, it provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis detects LMNB2 at approximately 68 kDa in HeLa, THP-1, and PC-3 cell lysates, with the slight difference from the predicted 70 kDa molecular weight likely reflecting post-translational processing typical of lamin proteins. Immunohistochemistry staining has been validated in human glioma and kidney tissue sections, while immunofluorescence studies in Jurkat cells reveal the characteristic nuclear envelope localization pattern. Flow cytometry analysis in HepG2 cells further confirms reliable detection in suspension-based assays.

Whether investigating nuclear lamina dynamics, exploring lamin alterations in cancer progression, or examining nuclear envelope breakdown during mitosis, this antibody provides a dependable tool for advancing your understanding of LMNB2 biology across diverse experimental contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Gene References into Functions

our studies strongly implicate an overarching role for Lamin B2 in the maintenance of nuclear architecture since loss of Lamin B2 relieves the spatial positional constraints required for maintaining conserved localization of aneuploid chromosome territories in the interphase nucleus. PMID: 26921073
Mutation of the nuclear lamin gene LMNB2 in progressive myoclonus epilepsy with early ataxia. PMID: 25954030
Nuclear envelope remodelling during human spermiogenesis involves somatic B-type lamins and a spermatid-specific B3 lamin isoform. PMID: 25477337
Treating normal human fibroblasts with farnesyltransferase inhibitors causes the accumulation of unprocessed lamin B2 and lamin A and a decrease in mature lamin B1 PMID: 23475125
Mutation in LMNB2 gene is associated with partial lipodystrophy. PMID: 22768673
Studies indicate that the lamin-binding proteins implicated in laminopathies include lamin B2 and nuclear envelope proteins. PMID: 21400569
These findings indicate that a lamin dimer principally has the freedom for a "combinatorial" head-to-tail association with all types of lamins, a property that might be of significant importance for the assembly of the nuclear lamina. PMID: 20004208
analysis of protein-DNA interactions at the human lamin B2 replication origin PMID: 12902329
The proteins bound in vivo at the LMNB2 replication origin were investigated along the cell cycle. PMID: 12912926
This analysis reveals the modular structure of the lamin B2 origin and supports the idea that sequence elements close to the replication start site play an important role in origin activation. PMID: 15024083
the distinctive ensemble of heterotypic lamin interactions in a particular cell type affects the stability of the lamin polymer PMID: 15284226
the lamin B2 origin adopts a structure partly composed of intramolecular TAT triads PMID: 15611042
lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors; propose that lamin B is a structural component of the spindle matrix that promotes microtubule assembly and organization in mitosis PMID: 16543417
Three new rare heterozygous mutations are the first reported for LMNB2 and are the first reported among patients with acquired partial lipodystrophy. PMID: 16826530
human lamin B(2) is indeed synthesized as a longer version than previously reported, because it contains these additional 20 amino acids PMID: 17070523
The human DNA replication origin, located in the lamin B2 gene, interacts with the DNA topoisomerases I and II in a cell cycle-modulated manner. PMID: 17290216

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Involvement in disease

Partial acquired lipodystrophy (APLD); Epilepsy, progressive myoclonic 9 (EPM9)

Subcellular Location

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.

Protein Families

Intermediate filament family

Database Links

HGNC: 6638

OMIM: 150341

KEGG: hsa:84823

STRING: 9606.ENSP00000462730

UniGene: Hs.538286

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Apoptosis

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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