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The creation of the LY6G6D recombinant monoclonal antibody involves the following steps: 1. LY6G6D antibody generation. Use the recombinant human LY6G6D protein as the immunogen to induce an immune reaction and harvest B cells. 2. Gene cloning. Extract total RNA from the harvested B cells. Convert the RNA into cDNA using reverse transcription. Amplify the LY6G6D antibody genes using PCR with primers specific to the antibody constant regions. Clone the antibody genes into an expression vector. 3. Recombinant antibody expression and purification. Transfect the expression vector containing the LY6G6D antibody genes into host cells for antibody expression. Collect the cell culture supernatant and purify the LY6G6D recombinant monoclonal antibody using affinity chromatography. 4. Antibody characterization and validation. This purified antibody has been tested to recognize and bind to the human and macaca fascicularis LY6G6D protein in ELISA and FC.
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