LY6G6D Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA013246MA1HU
Size	US$210
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Image	The Binding Activity of LY6G6D with anti-LY6G6D antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis LY6G6D(CSB-YP4607MOV) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody, the EC₅₀ is 3.963-7.154 ng/mL. The Binding Activity of LY6G6D with anti-LY6G6D antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human LY6G6D(CSB-YP013246HU) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody, the EC₅₀ is 2.816-3.741 ng/mL. Untransfected HEK293T cells surface (green line) and transfected Human LY6G6D HEK293T stable cells surface(red line) were stained with anti-LY6G6D antibody (CSB-RA013246MA1HU) (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
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Product Details
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Target Background

Product Details

Uniprot No.

O95868

Target Names

LY6G6D

Alternative Names

Lymphocyte antigen 6 complex locus protein G6d; Protein Ly6-D; Megakaryocyte-enhanced gene transcript 1 protein; LY6G6D; C6orf23; G6D; MEGT1; NG25

Species Reactivity

Human, Macaca fascicularis

Immunogen

Recombinant Human LY6G6D protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

8I8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, FC

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The creation of the LY6G6D recombinant monoclonal antibody involves the following steps: 1. LY6G6D antibody generation. Use the recombinant human LY6G6D protein as the immunogen to induce an immune reaction and harvest B cells. 2. Gene cloning. Extract total RNA from the harvested B cells. Convert the RNA into cDNA using reverse transcription. Amplify the LY6G6D antibody genes using PCR with primers specific to the antibody constant regions. Clone the antibody genes into an expression vector. 3. Recombinant antibody expression and purification. Transfect the expression vector containing the LY6G6D antibody genes into host cells for antibody expression. Collect the cell culture supernatant and purify the LY6G6D recombinant monoclonal antibody using affinity chromatography. 4. Antibody characterization and validation. This purified antibody has been tested to recognize and bind to the human and macaca fascicularis LY6G6D protein in ELISA and FC.

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Target Background

Gene References into Functions

Frequencies of the DRB1, DQA1, DQB1 and TNFA alleles in immigrant population of West Siberia PMID: 19807019

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Cell projection, filopodium.

Tissue Specificity

Expressed in the adult lung, and in fetal liver, lung, kidney, brain and spleen.

Database Links

HGNC: 13935

OMIM: 606038

KEGG: hsa:58530

STRING: 9606.ENSP00000364985

UniGene: Hs.591792

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Related pathways

Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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