Product Details

Uniprot No.

Target Names

Alternative Names

Microtubule-associated proteins 1A/1B light chain 3B (Autophagy-related protein LC3 B) (Autophagy-related ubiquitin-like modifier LC3 B) (MAP1 light chain 3-like protein 2) (MAP1A/MAP1B light chain 3 B) (MAP1A/MAP1B LC3 B) (Microtubule-associated protein 1 light chain 3 beta), MAP1LC3B, MAP1ALC3

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human MAP1LC3B

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MAP1LC3B, commonly known as LC3B, serves as one of the most widely used markers for monitoring autophagy, the essential cellular recycling process that maintains homeostasis by degrading damaged organelles and misfolded proteins. This protein plays a central role in autophagosome formation, and its conversion from the cytosolic LC3B-I form to the lipidated LC3B-II form provides researchers with a reliable readout of autophagic flux. Given autophagy's involvement in cancer progression, neurodegeneration, cardiovascular disease, and metabolic disorders, accurate detection of LC3B is fundamental to understanding these pathological processes.

This recombinant monoclonal antibody, clone 7D11, offers the reproducibility that autophagy research demands. Because the antibody sequence is defined and produced recombinantly in rabbit host, you can expect consistent performance across experiments and between lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations.

Western blot validation demonstrates reliable detection of LC3B at the expected 15 kDa molecular weight across a diverse panel of human cell lines, including HepG2 hepatocellular carcinoma, U87 and U251 glioblastoma, HeLa cervical carcinoma, and RAW264.7 macrophages. This broad validation across different cellular contexts confirms the antibody's utility for studying autophagy in cancer biology, immunology, and neuroscience applications. Flow cytometry validation in HeLa cells further extends experimental flexibility, enabling single-cell analysis of autophagy dynamics in mixed populations.

Whether you are investigating autophagy as a tumor survival mechanism, exploring its neuroprotective roles, or examining metabolic stress responses, this antibody provides the specificity and consistency needed for meaningful results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.

Gene References into Functions

Study demonstrated that the expression of LC3B was upregulated in 4nitroquinoline1oxideinduced oral carcinogenesis, accompanied with myeloidderived suppressor cells and regulatory T cells accumulation. PMID: 30272335
High p62 cytoplasmic expression on its own (p PMID: 29897944
Here the authors present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. PMID: 28442745
The results suggest that autophagy-associated proteins LC3A, LC3B, and Beclin-1 might be potential biomarkers for subclassification, differentiation, and local metastasis in primary lung tumor. PMID: 29545906
Simultaneous high expression of LC3B (and ULK1) had a poorer survival rate in hepatocellular carcinoma patients. PMID: 29091866
LC3B and ESRRA might be a useful prognostic factor in patients with Muscle-invasive bladder cancer. The co-expression of LC3B and ESRRA might be a prognostic and therapeutic target for patients with bladder cancer. PMID: 29599373
High levels of LC3B is associated with non-small cell lung cancer. PMID: 28558758
LC3b was significantly overexpressed in malignant compared to benign prostate tissue. However, positive LC3b immunoreactivity in PCa, as a marker of increased autophagy, was independently associated with a reduced disease-specific mortality. PMID: 28423666
Our results suggested that activated Akt/mTOR-autophagy may have a role in the local T cell-mediated immunoregulatory mechanism of oral lichen planus (OLP). LC3B might be a valuable marker to monitor the disease severity of OLP. PMID: 28482233
presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancer PMID: 26979828
the L341V mutation limits the critical step of SQSTM1 recruitment to the phagophore PMID: 27158844
LC3B and p62 have roles in autophagy in esophageal adenocarcinoma PMID: 27250034
Data indicate that tubule-associated protein 1 light chain 3 beta (LC3B) can be potentially useful for identifying autophagosomes and differentiating their developmental stages. PMID: 28506764
analysis of the RavZ and LC3 complex reveals the mechanism for deconjugation of LC3 on the phagophore membrane PMID: 27791457
SQSTM1 is ubiquitinated by NEDD4 while LC3 functions as an activator of NEDD4 ligase activity. PMID: 28085563
Cardiolipin interaction with various Atg8 human orthologs, namely LC3B, GABARAPL2 and GABARAP, was investigated. PMID: 27764541
Insights into links between autophagy and the ubiquitin system showed that LC3B-binding can steer intrinsic NEDD4 E3 ligase activity. PMID: 28470758
Determined is the three-dimensional crystal structures of LC3B in complex with three different LIR motifs of RavZ from Legionella pneumophila, an intracellular pathogen that can manipulate the host autophagy system. PMID: 28668392
We found that 25-epi Ritterostatin GN1N induced cell death in melanoma cells at nanomolar concentrations, and this cell death was characterized by inhibition of GRP78 expression, increased expression of the ER stress marker CHOP, loss of mitochondrial membrane potential, and lipidation of the autophagy marker protein LC3B. PMID: 28393217
Double IF showed the co-localization of AQP5 and LC3B on BafA1-treated heated cells. In conclusion, we demonstrated that heat shock decreased AQP5 on cellular membranes and in the cytoplasm by activating autophagic degradation, and heat shock and AQP5 knockdown exerted similar anticancer effects, suggesting that heat shock exerts anticancer effects via the autophagic degradation of AQP5. PMID: 28358429
structural and biochemical results reveal a working model for the specific recognition of FUNDC1 by LC3B and imply that the reversible phosphorylation modification of mitophagy receptors may be a switch for selective mitophagy. PMID: 27757847
Low expression of MAP1LC3B is associated with lymph node metastasis in gastric cancer. PMID: 27655288
Poly C binding protein 1 represses autophagy through downregulation of LC3B to promote tumor cell apoptosis in starvation PMID: 26880484
BAG3 maintains the basal amount of LC3B protein by controlling the translation of its mRNA in HeLa and HEK293 cells. PMID: 26654586
Among the 101 patients, the frequency of high expression of beclin-1 was 31.7% (32/101) and that of LC3b was 46.5% (47/101). A pathologic complete response was inversely associated with LC3b expression (P = 0.003) and alterations in the expression of autophagy-related proteins PMID: 26965179
Collectively, our findings indicate that MIR494 reduces cell survival in 769-P renal cancer cells which is accompanied by increased lipid droplet formation (which occurs in a LC3B-dependent manner) and mitochondrial changes. PMID: 26794413
Data show CGK733 induced microtubule associated protein LC3B formation upstream of AMP-activated protein kinase and protein kinase RNA-like endoplasmic reticulum kinase/CCAAT-enhancer-binding protein homologous protein pathways and p21 Cip1 expression. PMID: 26486079
the combined positivity for LC3B(+) puncta and nuclear HMGB1 is a positive predictor for longer BC survival PMID: 26506894
In microsatellite stable carcinomas, the level of LC3B-II expression was higher than that in the microsatellite unstable carcinomas. PMID: 26502823
Loss of HPS1 protein results in impaired autophagy that is restored by exogenous LC3B and that defective autophagy might therefore play a critical role in the development and progression of Hermansky-Pudlak syndrome. PMID: 26719147
mRNA levels of MAP1LC3B, an autophagic marker, showed a 5-fold decrease in symptomatic samples. PMID: 25503069
LC3B may promote the migration and invasion of EOC cells by affecting the cytoskeleton via the RhoA pathway. PMID: 25607473
This study unveils that HIV-1 Vif inhibits autophagy via interaction with LC3B independently of its action on APOBEC3G and, therefore, suggest a new function of this viral protein in restricting innate antiviral mechanisms. PMID: 25490467
Data show that interaction between promyelocytic leukemia protein (PML) and microtubule-associated protein light chain 3 (LC3) contributes to cell growth inhibition function of PML. PMID: 25419843
investigated the expression of autophagy-related markers microtubule-associated protein IA/IB light chain 3 (LC3) and p62/sequestosome-1 (p62), in cutaneous squamous cell carcinoma specimens and assessed their correlation to clinicopathological factors PMID: 24690104
When not bound to autophagosomes, LC3B associates with a multicomponent complex with an effective size of ~500 kDa in the cytoplasm. PMID: 24646892
positive fibroblastic LC3B correlates with lower invasion, and low expression of fibroblastic Cav-1 is a novel predictor of poor GC prognosis. PMID: 23203033
high expression of LC3B, correlated with vascular invasion and lymph node metastasis, might be a novel prognostic biomarker and would be a potential therapy target for HCC. PMID: 25256671
High intensity of LC3B staining was predictive of poor prognosis. PMID: 24900981
elevating the levels of TSC1 (tuberous sclerosis complex) and TSC2 and inactivating MTOR and RPS6KB/p70S6K, causing cleaved MAP1LC3B levels to increase PMID: 24113030
High cytoplasmic p62 expression accompanied with either a low or high LC3B expression. PMID: 24983366
Data indicate that high cytoplasmic microtubule-associated protein 1 light chain LC3A, LC3B, Beclin 1 and p62/SQSTM1 expressions were independently linked with the Gleason score. PMID: 23787295
LC3B can be used as a prognostic marker in patients with non-pCR after NCT for breast cancer, which highlights the importance of autophagy in the biologic behavior of chemoresistant cancer cells PMID: 24141623
knockdown of LC3B but not GABARAPs resulted in significant accumulation of p62/Sqstm1, one of the selective substrates for autophagy PMID: 24582747
The results of this study identify a new physiological role for the PSF-LC3B axis as a potential endogenous modulator of colon cancer treatment. PMID: 24288667
these data indicated that LC3B-II deacetylation, which was partly mediated by HDAC6, is involved in autophagic degradation during serum starvation. PMID: 24220335
Beclin-1 and LC3-II are downregulated in hypopharyngeal squamous cell carcinoma patients, and their aberrant expression correlates with poor prognosis. PMID: 23935917
NMR and crystal structures of the autophagy modifier LC3B in complex with the LC3 interaction region of optineurin. PMID: 23805866
These preliminary results demonstrated that high LC3B expression was associated with lymph node and distant metastasis in triple-negative breast cancer PMID: 23371253
Data show that VPRBP (viral protein R-binding protein)-LC3B (light-chain 3B)/p62(SQSTM1) were in the same protein complex. PMID: 22963397

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Subcellular Location

Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor.

Protein Families

ATG8 family

Tissue Specificity

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

Database Links

HGNC: 13352

OMIM: 609604

KEGG: hsa:81631

STRING: 9606.ENSP00000268607

UniGene: Hs.356061

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Code	CSB-RA551427A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,RAW264.7 whole cell lysate,U251 whole cell lysate,Hela whole cell lysate All lanes: LC3B antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa Overlay Peak curve showing Hela cells stained with CSB-RA551427A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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MAP1LC3B Recombinant Monoclonal Antibody

Product Details

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Target Background