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MAP2K3 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA953665A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate(20µg),SH-SY5Y whole cell lysate(20µg), HepG2 whole cell lysate(20µg), NIH/3T3 whole cell lysate(20µg),HEK293 whole cell lysate(20µg),Hela whole cell lysate(20µg),U-251MG whole cell lysate(20µg)
    All lanes: MEK3 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 39 kDa
    Observed band size: 36,39 kDa
    Exposure time：30s
  • Overlay Peak curve showing PC-3 cells stained with CSB-RA953665A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P46734
Target Names
MAP2K3
Alternative Names
AW212142 antibody; dual specificity mitogen activated protein kinase kinase 3 antibody; Dual specificity mitogen-activated protein kinase kinase 3 antibody; MAP kinase kinase 3 antibody; map2k3 antibody; MAPK ERK kinase 3 antibody; MAPK/ERK kinase 3 antibody; MAPKK 3 antibody; MAPKK3 antibody; MEK 3 antibody; MEK3 antibody; Mitogen activated protein kinase kinase 3 antibody; MKK 3 antibody; MKK3 antibody; mMKK3b antibody; MP2K3_HUMAN antibody; PRKMK 3 antibody; PRKMK3 antibody; protein kinase, mitogen-activated, kinase 3 antibody; SAPK kinase 2 antibody; SAPKK 2 antibody; SAPKK2 antibody; Stress activated protein kinase kinase 2 antibody
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human MAP2K3
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
14G8
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

MAP2K3, also known as MEK3 or MKK3, serves as a critical upstream kinase in the p38 MAPK signaling cascade, playing essential roles in cellular stress responses, inflammation, and apoptosis. As a dual-specificity kinase that phosphorylates both threonine and tyrosine residues on p38 MAPK, MAP2K3 has become an important target for researchers investigating stress-activated signaling pathways, inflammatory diseases, and cancer biology.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human MAP2K3, offers the consistency and reliability that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect reproducible results across different lots, eliminating a common source of experimental variability that can complicate long-term studies or multi-site collaborations.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody successfully detects MAP2K3 in a diverse panel of human cell lines including K562, SH-SY5Y, HepG2, HEK293, HeLa, and U-251MG, as well as mouse NIH/3T3 cells, confirming cross-species reactivity with both human and mouse samples. The observed bands at 36 and 39 kDa align with the predicted molecular weight, with the slight size variation likely reflecting post-translational modifications or alternative isoforms. Flow cytometry validation using PC-3 cells demonstrates clear positive signal separation from isotype controls, providing researchers with flexibility for intracellular protein quantification studies.

Whether investigating MAPK signaling dynamics, screening for pathway modulators, or characterizing stress response mechanisms, this antibody provides a dependable tool for advancing your research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Gene References into Functions
  1. Advanced glycation end products significantly activated ASK1, MKK3, and MKK6, which led to activation of p38 MAPK, resulting in upregulated fibrotic response in human coronary smooth muscle cells. PMID: 30305582
  2. The study identifies MKK3 as a negative regulator of mitochondrial function and inflammatory responses to cigarette smoke and suggests that MKK3 could be a therapeutic target. PMID: 27717867
  3. High MKK3 expression is associated with lung cancer. PMID: 28628118
  4. miR-21 targets MKK3 in vivo and in vitro, inhibiting the downstream factors IL-6 and TNF-alpha, in ischemia pretreatment protection from ischemia-reperfusion induced kidney injury. PMID: 26149640
  5. MKK3 overexpression upregulated the cyclin-dependent kinase inhibitors, p16 INK4A and p15 INK4B in hepatocellular carcinoma cells was Bim1, was downregulated following MKK3 overexpression. PMID: 26573508
  6. Our results suggest that asthma is associated with MKK3 over-expression in CD8+ cells; we have also demonstrated that MKK3 may be critical for airway neutrophilia PMID: 24480516
  7. MicroRNA-21 promotes hepatocellular carcinoma HepG2 cell proliferation through repression of mitogen-activated protein kinase-kinase 3. PMID: 24112539
  8. study detected higher MKK3 activation in isolated peripheral blood mononuclear cells from septic patients compared with nonseptic controls PMID: 24487387
  9. study concludes MAP2K3 is a reproducible obesity locus that may affect body weight via complex mechanisms involving appetite regulation and hypothalamic inflammation PMID: 23825110
  10. miR-20a acts in a feedback loop to repress the expression of MKK3 and to negatively regulate the p38 pathway-mediated VEGF-induced endothelial cell migration and angiogenesis PMID: 22696064
  11. the balance between MKK6 and MKK3 mediates p38 MAPK associated resistance to cisplatin in NSCLC PMID: 22164285
  12. LFA-1-induced stabilization of ARE-containing mRNAs in T cells is dependent on HuR, and occurs through the Vav-1, Rac1/2, MKK3 and p38MAPK signaling cascade PMID: 21206905
  13. MAP2K3 is identified as a protein to promote senescence in human breast epithelial cells. PMID: 21137025
  14. Data provide evidence that p38 Map kinase (MAPK) pathway is activated leading to increased upregulation of mixed lineage kinase 3, MKK3/6, MSK1, and Mapkapk2, upon treatment of BCR/ABL expressing cells with dasatinib. PMID: 19672773
  15. role in activating Mirk protein kinase PMID: 11980910
  16. interacts with phospholipase c-beta 2 PMID: 12054652
  17. role in pathway that promotes urokinase plasminogen activator mRNA stability in invasive breast cancer cells PMID: 12377770
  18. TAK1- and MKK3-mediated activation of p38 are facilitated by Smad7 PMID: 12589052
  19. MKK3 is selectively activated by the new subfamily of Ste20-like kinases. PMID: 13679851
  20. a specific requirement for p150(Glued)/dynein/functional microtubules in activation of MKK3/6 and p38 MAPKs in vivo. PMID: 15375157
  21. MAP kinase kinase3- and 6-dependent activation of the alpha-isoform of p38 MAP kinase is required for the cytoskeletal changes induced by neutrophil adherence and influences subsequent neutrophil migration toward endothelial cell junctions PMID: 15516490
  22. H-Ras-specific activation of Rac-MKK3/6-p38 pathway has a role in invasion and migration of breast epithelial cells PMID: 15677464
  23. MKK3 and MKK6 make individual contributions to p38 activation in fibroblast-like synoviocytes after cytokine stimulation PMID: 15778394
  24. p38 mediates EGF receptor activation after oxidant injury; Src activates MMK3, which, in turn, activates p38; and the EGF receptor signaling pathway plays a critical role in renal epithelial cell dedifferentiation PMID: 15797859
  25. Bax is phosphorylated by stress-activated JNK and/or p38 kinase and phosphorylation of Bax leads to mitochondrial translocation prior to apoptosis PMID: 16709574
  26. Mitogen-activated protein kinase (MAPK) kinase 3 (MKK3) is a key activator of p38 MAPK in glioma; MKK3 activation is strongly correlated with p38 activation in vitro and in vivo. PMID: 17406030
  27. Cytokine activation of MAPK14 and apoptosis is opposed by ACTN4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3. PMID: 17438131
  28. as a mediator of SF- and Src-stimulated NF-kappaB activity. Finally, the Src/Rac1/MKK3/6/p38 and Src/TAK1/NF-kappaB-inducing kinase pathways exhibited cross-talk at the level of MKK3. PMID: 19047046

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Involvement in disease
Defects in MAP2K3 may be involved in colon cancer.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Database Links

HGNC: 6843

OMIM: 602315

KEGG: hsa:5606

STRING: 9606.ENSP00000345083

UniGene: Hs.514012

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Related pathways
MAPK signaling pathway TNF signaling pathway Cellular senescence Fc epsilon RI signaling pathway Toll-like receptor signaling pathway Rap1 signaling pathway
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