MCM6 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA986091A0HU
Size	US$210
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Image	IHC image of CSB-RA986091A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA986091A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA986091A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q14566

Target Names

MCM6

Alternative Names

DNA replication licensing factor MCM6 antibody; MCG40308 antibody; Mcm6 antibody; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog; S. pombe) antibody; MCM6 minichromosome maintenance deficient 6 antibody; MCM6_HUMAN antibody; Minichromosome maintenance complex component 6 antibody; Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody; Minichromosome maintenance deficient 6 homolog antibody; Minichromosome maintenance protein 6 antibody; Mis5 antibody; MIS5 homolog antibody; p105MCM antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MCM6

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14E12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MCM6 serves as an essential component of the minichromosome maintenance complex, a hexameric helicase critical for DNA replication licensing and elongation. This protein ensures that DNA replication occurs only once per cell cycle by loading onto replication origins during the G1 phase and facilitating origin firing during S phase. Dysregulation of MCM6 has been implicated in various malignancies, making it a valuable marker for studying cell proliferation and cancer biology.

This recombinant monoclonal antibody, clone 14E12, offers the reproducibility and consistency that demanding research applications require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, raised against a synthetic peptide derived from human MCM6, provides reliable target recognition across multiple experimental platforms.

Validation studies demonstrate robust performance in immunohistochemistry, where the antibody successfully detects MCM6 in paraffin-embedded human cervical cancer and lymph node tissues at 1:100 dilution using citrate buffer antigen retrieval. For researchers investigating cell cycle dynamics, flow cytometry analysis in Jurkat cells confirms intracellular detection capability, showing clear positive signal separation from isotype controls when cells are fixed and permeabilized.

The affinity-purified formulation arrives ready for use in ELISA, IHC, and flow cytometry applications, with recommended working dilutions of 1:50-1:200 for tissue and cell-based assays. Whether investigating replication stress responses, characterizing proliferative indices in tumor samples, or exploring MCM complex biology, this antibody provides a dependable tool for advancing your understanding of DNA replication control mechanisms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Gene References into Functions

Associations of the MCM6-rs3754686 proxy for milk intake in Mediterranean and American populations with cardiovascular biomarkers, disease and mortality: Mendelian randomization. PMID: 27624874
MCM6 is a highly reproducible marker of poor prognosis in endometrial cancer PMID: 29243125
genetic association studies in population in Western Europe: Data confirm an SNP in MCM6 (rs4988235) is predictive of milk consumption; lack of association of genetically predicted milk consumption with prevention of osteoporosis, ischemic heart disease, or type 2 diabetes suggests few beneficial effects of milk consumption but is more consistent with milk promoting adiposity/overweight. PMID: 28225053
Methyl(R217)HuR and MCM6 are inversely correlated and are prognostic markers in non small cell lung carcinoma PMID: 26013954
Report presence of homozygous--13910CC genetic variant in the MCM-6 gene upstream from the lactase gene in lactose intolerant patients treated with lactose-restricted diet. PMID: 25651706
Results indicate that the interaction between hCdt1 and hMcm6 through their interacting domains is key for hCdt1 in facilitating the MCM hetero-hexamer to load onto chromatin for replication licensing. PMID: 21099365
The subtype and MCM6 protein expression in craniopharyngiomas are related to the prognosis of tumor. PMID: 17294730
the molecular mechanism of Mcm2-7 chromatin loading and prereplicative complex assembly. PMID: 20202939
The numbers of the three genotypic combinations reveal that the percentage of this population that is genetically lactose intolerant is 8.23% and that the allele frequencies found are consistent with the expected Mendelian distribution. PMID: 20173814
Data show that cells subjected to chronic hypoxia the replicative restart was inhibited along with numerous replication factors including MCM6 and RPA, and cells reoxygenated after acute hypoxia underwent rapid p53-dependent apoptosis. PMID: 20103649
It was found that the MCM6 label index (LI) was significantly higher in adamantine epithelioma than squamous papillary tumor, and in both primary tumor subtypes the LIs of tumors with recurrence were higher than those without recurrence. PMID: 17410335

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Subcellular Location

Nucleus. Chromosome.

Protein Families

MCM family

Database Links

HGNC: 6949

OMIM: 223100

KEGG: hsa:4175

STRING: 9606.ENSP00000264156

UniGene: Hs.444118

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cell cycle

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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