Product Details

Uniprot No.

Target Names

Alternative Names

CDABP0042 antibody; CDC 47 antibody; CDC47 antibody; CDC47 homolog antibody; Cdc47; S. cerevisiae; homolog of antibody; DNA replication licensing factor MCM7 antibody; Homolog of S. cerevisiae Cdc47 antibody; MCM 2 antibody; MCM 7 antibody; MCM2 antibody; MCM2; formerly antibody; Mcm7 antibody; MCM7 minichromosome maintenance deficient 7 antibody; MCM7_HUMAN antibody; Minichromosome Maintainence 7 antibody; Minichromosome maintainence; S. cerevisiae; homolog of antibody; Minichromosome maintenance complex component 7 antibody; Minichromosome maintenance deficient 7 antibody; Minichromosome maintenance protein 7 antibody; P1.1 MCM3 antibody; P1.1-MCM3 antibody; P1CDC47 antibody; P85MCM antibody; PNAS 146 antibody; PNAS146 antibody; PPP1R104 antibody; Protein phosphatase 1; regulatory subunit 104 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MCM7

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16D6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MCM7 serves as an essential component of the minichromosome maintenance complex, a hexameric helicase critical for DNA replication licensing and initiation. This protein ensures that genomic DNA is replicated exactly once per cell cycle, making it a key marker for studying cell proliferation, cancer biology, and DNA replication dynamics. Dysregulation of MCM7 has been implicated in various malignancies, positioning it as both a research target and potential diagnostic marker.

This recombinant monoclonal antibody, clone 16D6, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human MCM7 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity with minimal lot-to-lot variation. Affinity chromatography purification ensures high purity for reliable experimental outcomes.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis detects MCM7 in diverse human cell lines including HEK293, HeLa, Jurkat, and COLO205 lysates at dilutions ranging from 1:500 to 1:2000. The observed band at approximately 100 kDa, compared to the predicted 81 kDa molecular weight, likely reflects post-translational modifications such as phosphorylation or other regulatory modifications characteristic of this actively regulated protein. Immunohistochemistry validation in paraffin-embedded human gastric cancer and lymph node tissues confirms utility for tissue-based studies at 1:50 to 1:200 dilutions. Immunofluorescence staining in HeLa cells reveals clear nuclear localization consistent with MCM7's role in DNA replication.

This antibody supports researchers investigating cell cycle regulation, replication stress responses, and tumor proliferation markers, offering flexibility across biochemical, histological, and cell-based experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Gene References into Functions

Our results indicate that MCM7 may exert certain functions on spindle formation to prevent cytokinesis during early mitosis by regulating CDK1 activity. PMID: 28588300
MCM7 is helpful for us to make differential diagnosis in pathological grade, MCM7 combination of Ki67 may serve as more sensitive proliferation markers for evaluation of gastric carcinoma and precancerous lesions PMID: 28540486
CDT1, MCM7, and NUDT1 were shown to be up-regulated in hepatocellular carcinoma tissues and provide a more accurate diagnosis than alpha-fetal protein alone. PMID: 29442275
High expression of MCM-7 was reduced in BVP-treated cancer cells. PMID: 28628830
Data indicate that RACK1 increases interactions between Akt and MCM7 and promotes Akt-dependent MCM7 phosphorylation, which in turn increases MCM7 binding to chromatin and miniature chromosome maintenance (MCM) complex formation. PMID: 28465488
our findings suggested that MCM7 promoted tumor cell proliferation, colony formation and migration of ESCC cells via activating AKT1/mTOR signaling pathway PMID: 28498460
Results proved that MCM7 proliferative index is a better method for identifying patients with risk of recurrence in patients surgically resected for meningiomas compared with the traditional methods used in the current clinical practice. PMID: 27868157
MCM7-cyclin D1 pathway may participate in cancer progression. PMID: 28182015
The MCM7 directly binds to the centrosomal linker protein Cep68 in vitro and complexes with Cep68 and VHL in vivo. PMID: 28578000
The results clearly demonstrate 7q21-22 amplification, MCM7, and its intronic miR-25 are the major molecular switches involved in the complex oncogenic circuits of gastric cancer. PMID: 28059478
High MCM7 expression is associated with non-small cell lung cancer. PMID: 27797825
Results show that the expression of MCM7 gene and its microRNA, miR-106b-25 cluster is reduced under hypoxia. PMID: 28668890
Authors found that MCM7 highly expressed in K562 cells rather than that in normal neutrophils. Thus, lentivirus-mediated shRNA targeting MCM7 was used to suppress its endogenous expression in K562 cells and develop a novel therapeutic strategy for leukemia. PMID: 28058629
MCM7 polymorphisms were associated with survival rate and prognosis in acute myeloid leukemia patients. PMID: 27837251
The BCL2, MCM7, and CCNE1 genes might play distinctive roles in cisplatin resistance in bladder cancer. PMID: 26966728
High MCM-7 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
KCTD5 is not involved in polyubiquitylation of MCM7 replication factor.Formation of trimeric complexes of KCTD5/cullin3 with MCM7, ZNF711 and FAM193B. PMID: 26188516
MCM7 and G9a may serve as effective prognostic factors and could also be used as biomarkers for predicting various clinical outcomes of oesophageal squamous cell carcinoma PMID: 24805087
MCM7 is essential for RNA splicing of epidermal growth factor receptor, c-Met, and platelet-derived growth factor receptor PMID: 25425645
MCM7 expression may be a useful prognosis indicator in patients with diffuse-type gastric adenocarcinoma and its knockdown by RNA interference quenches its oncogenic property in gastric cancer PMID: 24647462
We observed that lower levels of MCM7 correlate with reduced cell proliferation, lower autophagic capacity, and higher intracellular protein abundance phenotypes of aged and systemic sclerosis cells. PMID: 24496236
Knockdown of MCM7 expression reduces glioblastoma multiforme cell proliferation and inhibits xenograft tumor growth in mice. PMID: 24166506
expression of MCM7 is an independent risk factor for recurrence-free survival in patients with Dukes C colorectal cancer PMID: 25075101
The A to G base change in SNP rs999885 of MCM7 may provide a protective effect on the prognosis of intermediate or advanced hepatocellular carcinoma in the Chinese. PMID: 24416400
MCM 7, geminin and topo IIalpha can be reliable tools for the differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells. PMID: 23860238
MCM7 is a substrate of cyclin E/Cdk2 and can be phosphorylated on Ser-121. PMID: 23720738
EGFR enhances MCM7 phosphorylation and DNA replication through Lyn phosphorylation in human cancer cells. PMID: 23764002
MCM7 interacts with the receptor for activated protein kinase C 1 (RACK1), a protein kinase C (PKC) adaptor, in vivo and in vitro. PMID: 23313748
Protein levels of MCM7 and p63 were associated significantly with high-grade cervical lesion, and aberrant p63 protein level may distinguish different histopathologic types of cervical carcinoma. PMID: 23318911
the expression of cyclin D1, MCM7, TRIM29, and UBE2C was found to be significantly associated with progression to muscle-invasive bladder cancer. PMID: 23201130
MCM7 expression may be a useful predictor of prognosis in patients with hepatocellular carcinoma after resection. PMID: 22784096
MCM7 and Ki67 in tumor tissues may be potential markers of a poor prognosis for non-small cell lung cancer patients PMID: 22456526
elevated expression of MCM7 to be associated with poor prognosis for patients with non-small cell lung cancer PMID: 21619671
low level of MCM7 of proteins expression which may explain commonly known low mitotic activity of desmoid tumour cells PMID: 21478101
MCM7 expression was significantly higher in high-grade serous carcinomas than in serous borderline tumors or other histological subtypes of ovarian cancer. PMID: 21076460
Results demonstrate that interaction with Mcm7 is essential for the function of cyclin A in promoting S-phase entry. PMID: 21078875
distribution pattern is similar to Ki-67;can be used as proliferation marker in experimental neoplasms PMID: 20048674
Transcriptional activation of Mcm7 is mediated by HAT recruitment to the promoter region upon C. sinensis excretory-secretory products treatment. PMID: 20236609
uncovered a proto-oncogenic miRNA-dependent network for PTEN regulation and defined the MCM7 locus as a critical factor in initiating prostate tumorigenesis PMID: 20388916
MCM7 , critical for cell proliferation and maintenance of genome stability, are markedly down-regulated, Data hypothesized that their DNA-related functions could be partially limited in TRAIL-resistant HL-60 cells. PMID: 19834905
Minichromosome maintenance protein MCM7 is a direct target of the MYCN transcription factor in neuroblastoma. PMID: 11861392
Overexpressed MCM7 protein supports efficient DNA replication of Epstein-Barr virus oriP and rapid formation of tumors in nude mice without altering the activity of cellular DNA replication. PMID: 14550576
MCM7 may be a highly informative biomarker for cervical cancer. PMID: 14678972
hMCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells. PMID: 15538388
interaction between endogenous Plk1 and Mcm7 was detected in a soluble chromatin fraction. These findings suggest a new function for Plk1 in coordination of DNA replication and mitotic events. PMID: 15654075
MCM7 was found to be a more reliable and useful marker than Ki67 in assessing tumour proliferation and in the prognosis of patients. PMID: 15720416
MCM7 amplification is responsible for markedly increased DNA synthesis, cell proliferation and an increased cell invasion in prostate cancer. PMID: 16247466
p27Kip1 binds the conserved minichromosome maintenance domain of MCM7 proteins in a growth factor-dependent manner, such that the carboxyl terminal domain of p27Kip1 inhibits DNA replication independent of its function as a cyclin-dependent kinase PMID: 16289477
MCM7, the c-myc-regulated gene is involved in genotype-C-HBV-related HCC, suggesting that c-myc is related to the hepatocarcinogenic activity of genotype-C HBV. PMID: 16703398
Increase of the MCM7 is associated with tumor aggressiveness in astrocytoma PMID: 17094475

Hide All

Subcellular Location

Nucleus. Chromosome.

Protein Families

MCM family

Database Links

HGNC: 6950

OMIM: 600592

KEGG: hsa:4176

STRING: 9606.ENSP00000307288

UniGene: Hs.438720

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Code	CSB-RA237021A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), HeLa whole cell lysate(30µg), Jurkat whole cell lysate, COLO205 whole cell lysate(30µg) All lanes: MCM7 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 3min IHC image of CSB-RA237021A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA237021A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA237021A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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MCM7 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background