Code | CSB-RA984483A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
FC | 1:50-1:200 |
The process of generating a recombinant monoclonal antibody against MKI67 began with the immunization of a rabbit using a synthesized peptide from human MKI67 protein. B cells were subsequently isolated from the immunized rabbit, and RNA was extracted from these B cells. The extracted RNA was reverse-transcribed into cDNA, which was utilized as a template for amplifying MKI67 antibody genes using degenerate primers. These amplified MKI67 antibody genes were then integrated into a plasmid vector and introduced into host cells for expression. The MKI67 recombinant monoclonal antibody was then purified from the cell culture supernatant through affinity chromatography and subjected to ELISA, IHC, and FC applications, displaying specific reactivity with human MKI67 protein.
The main function of the MKI67 protein, also known as Ki-67, is as a cellular marker for cell proliferation. MKI67 is not directly involved in regulating the cell cycle or cell proliferation itself but serves as a valuable marker for assessing the proliferative activity of cells. Its primary function is to indicate whether a cell is in an active, proliferative state.
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