Code | CSB-RA187479A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The creation of the MME recombinant monoclonal antibody involves a meticulous process to ensure its exceptional quality and specificity. It begins with the isolation of B cells from the spleen of an immunized animal, where the synthesized peptide derived from human CD10 serves as the immunogen. RNA is then extracted from the B cells and converted into cDNA through reverse transcription. The MME antibody genes are amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells to facilitate the production of the MME recombinant monoclonal antibody. Following an appropriate incubation period, the antibody is harvested from the cell culture supernatant and undergoes meticulous purification using affinity chromatography, resulting in a highly purified form suitable for various applications. Rigorous characterization assays, including ELISA and IHC analysis, are conducted to confirm the antibody's specificity and functionality in detecting human MME protein. Through this rigorous production process, a reliable and effective MME recombinant monoclonal antibody is obtained, serving as a valuable tool in diverse research related to MME.
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