Recombinant Human Neprilysin (MME), partial

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Code	CSB-YP014653HU
Abbreviation	Recombinant Human MME protein, partial
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP014653HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) MME. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP014653HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) MME.
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

MME

Uniprot No.

P08473

Research Area

Cancer

Alternative Names

Atriopeptidase; CALLA; CD10; CD10 antigen; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase); Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); Membrane metallo endopeptidase; Membrane metallo endopeptidase variant 1; Membrane metallo endopeptidase variant 2; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 ; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; NEP_HUMAN; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase 24.11; Neutral endopeptidase; Neutral endopeptidase, membrane-associated; SFE; Skin fibroblast elastase

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

52-750aa

Target Protein Sequence

YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

81.8kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Presenting our premium Recombinant Human MME protein, an essential tool for researchers focused on cancer studies. Neprilysin (CD10) is expressed in yeast, and this recombinant protein encompasses the extracellular domain (52-750aa), providing superior performance for a wide range of applications in cancer research.

Engineered with an N-terminal 6xHis-tag, our Recombinant Human MME protein allows for seamless purification and detection. Boasting a purity of greater than 90% as determined by SDS-PAGE, you can confidently rely on the exceptional quality of this protein to enhance your cancer research endeavors. Available in both liquid and lyophilized powder formats, choose the most suitable form to meet your experimental needs and optimize your research workflow with our Recombinant Human MME protein.

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Target Background

Function

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Gene References into Functions

showed CD10 positivity in fibroblast-like stromal cells and fibrous material PMID: 29791034
these data indicated under cigarette smoke condensate treatment; losing of membrane p120ctn could upregulate surface NEP protein level and thus facilitate BEAS-2B cell migration. PMID: 30249887
High expression of CD10 is associated with lymph node invasion in colorectal cancer. PMID: 29653092
We observed an increase of VCAM-1 urine levels in diabetic nephropathy-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria PMID: 29854824
Degradation of tropoelastin and skin elastin by neprilysin PMID: 29196110
Xanthorrhizol (Xan) reduced 4-hydroxynonenal (HNE) levels on NEP proteins and preserved enzymatic activities of neprilysin (NEP) in HNE- or oligomeric Abeta42-treated cells. Xan reduced Abeta42 accumulation and protected neurones against oligomeric Abeta42-induced neurotoxicity through preservation of NEP activities PMID: 29330223
These findings support the role of the stromal CD10 expression in breast cancer progression and dissemination, and suggest a relationship with cancer stem cells. PMID: 29306324
Aberrant CD10 and BCL6 expression defines a subset of MCLs with higher mean Ki-67 index and higher prevalence of MUM1 expression PMID: 28628241
Our study firstly confirmed the association of MME miRNA binding site polymorphism with the risk of LOAD. However, the association results warrant further validation. PMID: 28294061
the Ras signaling pathway is involved in HIV-1 Tat-induced changes in ZO-1 and NEP. PMID: 28553432
In this meta-analysis of the Han Chinese population, neprilysin confers genetic susceptibility to Alzheimer's disease in Han Chinese populations. PMID: 26362309
High CD10 expression is associated with lymphoma in Waldeyer ring. PMID: 27616053
Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies PMID: 27588448
CD10 down expression in follicular lymphoma correlates with gastrointestinal lesion involving the stomach and large intestine PMID: 27513891
High CD10 expression is associated with Basal cell carcinoma of the skin. PMID: 27039776
the dynamic behavior of human NEP and NEP2 proteins was monitored by conducting molecular dynamics (MD) simulations. PMID: 26846903
This study reveals some unusual findings in otherwise classical disease entity, like absence of palpable spleen, presence of lymphadenopathy, normal or elevated leukocyte counts, expression of CD10, which at times could be diagnostically challenging. PMID: 26609034
Case Report: endometrial mixed carcinoma with the neuroendocrine component expressing CD10 that showed a long survival. PMID: 26830028
CD10 and Bcl2 expression in tumor cells could give convincing diagnostic value to distinguish squamous cell carcinoma from seborrheic keratosis PMID: 26573127
The peptide qf-Abeta(12-18)C (sequence VHHQKLVC) was cleaved by multiple Abeta-degrading enzymes, including NEP, ACE, and ECE-1, whereas the redesigned peptide qf-Abeta(12-16)AAC (sequence VHHQKAAC) was sensitive to NEP and ACE only. PMID: 27096746
Loss-of-function MME mutations are the most frequent cause of adult-onset autosomal-recessive Charcot-Marie-Tooth disease type 2 in Japan. PMID: 26991897
CD10 positivity is luminal/membranous in most benign apocrine breast lesions, the staining being nonuniversal and sometimes focal; analogous staining in apocrine malignancies seems rarer in DCIS and even rarer in invasive apocrine carcinomas, but atypical cytoplasmic positivity may also occur PMID: 26562027
These findings indicate that CD10 may promote tumor progression by regulating the expression profiles of genes related to cell proliferation, angiogenesis, and resistance to apoptosis. PMID: 26881775
CD10 gene expression plays a role in the pathogenesis of diffuse large B-cell lymphoma. PMID: 26414904
High tumoral CD10 expression correlates with aggressive histology in patients with malignant pleural mesothelioma. PMID: 25608772
In stage I lung adenocarcinoma, tumoral CD10 correlated with high-grade histology and was an independent predictor of recurrence in intermediate-grade tumors. PMID: 26141216
CD10 strongly labelled only the gastrointestinal cells, with a well-defined apical membrane signal PMID: 24754336
High CD10 expression is associated with Phylloides Tumors. PMID: 25921112
The expression of neprilysin is increased in glioma cells following 5-HT2C activation. PMID: 25452160
CD10 expression is related to a distinct gene expression signature in mantle cell lymphoma cases, but is without clinical or biological implications. PMID: 26124315
Correlation between CD10 stromal expression and disease-free survival rate in breast cancer patients. PMID: 23575921
E-cadherin and CD10 in endometrial lesions is not correlated, but reduced expression of both molecules could be critical for progression of endometrial carcinoma PMID: 25282623
Evaulate CD10 and mucin expression in relation to microsatellite instability/mismatch repair proteins in 47 cases of small bowel adenocarcinoma. PMID: 25759539
This meta-analysis indicates that rs3736187 (A/G) polymorphisms may be a potential beneficial single nucleotide polymorphism (SNP), which are associated with a decreased risk in Alzheimer disease. PMID: 25125048
Follicular lymphoma CD10(pos) follicular helper T cells specifically display an IL-4(hi)IFN-gamma(lo) cytokine profile and encompass the malignant B-cell-supportive follicular helper T cells subset. PMID: 25733581
Letter: suggest that CD10 is not a useful marker to differentiate seminoma from non-seminomatous germ cell tumours. PMID: 25713420
This study gives substantial proof to the various models/research papers explaining the role of CD10 in breast cancer pathogenesis. PMID: 25308002
Immunohistochemical distinction of renal cell carcinoma from other carcinomas with clear-cell histomorphology: utility of CD10 and CA-125 in addition to PAX-2, PAX-8, RCCma, and adipophilin. PMID: 25279712
Serum CD10 levels might serve as a useful marker of synchronous and metachronous liver metastasis in colorectal cancer. PMID: 24972738
PKCepsilon activation may have therapeutic efficacy for AD by reducing neurotoxic Abeta accumulation as well as having direct anti-apoptotic and synaptogenic effects PMID: 24848988
High CD10 expression is associated with squamous cell carcinoma. PMID: 24895167
MME expression level was differentially altered in Crohn disease and ulcerative colitis patients. PMID: 23827863
the recombinant brain-targeted neprilysin, ASN12, may be an effective treatment for AD and warrant further investigation in clinical trials. PMID: 24825898
Expression of CD10 is associated with therapeutic resistance and cancer stem cell-like properties of head and neck squamous cell carcinoma. PMID: 24874475
A direct up-regulation of stroma fibroblast MME expression under hypoxia might contribute to enhanced aggressiveness of hypoxic cancers. PMID: 24460801
Data indicate an important, previously neglected, role of NEP for regulation of luminal factors in the epididymis and suggest a novel role for CNP/guanylyl cyclase B in the epididymal epithelium. PMID: 24099862
only seminomas and intratubular germ cell neoplasia, the precursors of germ cell tumours, express CD10 PMID: 23857215
study suggests that the severity of periodontal disease may be associated with the expression of metalloendopeptidase genes, including NEP, ECE1 and ADAM17, in the buccal mucosal epithelium. PMID: 23360525
CD10 might be involved in the development of endometriosis due to its influence on CD44-dependent cell adhesion. PMID: 23653392
Overexpression of membrane metalloendopeptidase inhibits substance P stimulation of cholangiocarcinoma growth. PMID: 24603459