Product Details

Uniprot No.

Q96DH6

Target Names

MSI2

Alternative Names

RNA-binding protein Musashi homolog 2 (Musashi-2), MSI2

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human MSI2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MSI2 (Musashi-2) is an RNA-binding protein that plays a critical role in post-transcriptional gene regulation, stem cell maintenance, and cell fate determination. Its involvement in hematopoietic stem cell function and aberrant expression in various malignancies, particularly aggressive leukemias, has made MSI2 an important target for researchers investigating stem cell biology, cancer progression, and translational control mechanisms.

This recombinant monoclonal antibody offers the reproducibility and consistency that demanding research applications require. Generated from a defined sequence and produced using recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental conditions remain stable across long-term studies and multi-site collaborations.

Validation studies demonstrate robust performance across multiple experimental platforms. In western blot applications, the antibody reliably detects MSI2 at approximately 36 kDa—slightly above the predicted 35 kDa molecular weight, a difference attributable to post-translational modifications common to RNA-binding proteins. Detection has been confirmed across a diverse panel of human cell lines including SH-SY5Y neuroblastoma, U-251MG glioblastoma, HT-29 colorectal adenocarcinoma, HeLa cervical carcinoma, and K562 chronic myelogenous leukemia cells. Importantly, cross-species reactivity extends to mouse and rat brain tissue lysates, supporting translational research using rodent models. Flow cytometry validation in HeLa cells confirms utility for single-cell analysis of intracellular MSI2 expression.

Whether you are investigating MSI2's role in neural development, characterizing its expression in hematological malignancies, or exploring RNA regulatory networks, this antibody provides the validated performance and experimental flexibility needed for rigorous, reproducible research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

Gene References into Functions

This provides proof of concept for the development of Gn-lip as a molecular therapy for colon cancer with MSI1/MSI2 overexpression. PMID: 30097032
MSI2 expression is increased in epithelial cells adjacent to breast carcinoma, and increases with increasing proximity. PMID: 29093438
MSI2 expression was markedly increased in both PDAC cell lines and human PDAC specimens, and high MSI2 expression was associated with poor prognosis for PDAC. Forced MSI2 expression promoted PDAC proliferation, migration, and invasion in vitro and growth and metastasis in vivo, whereas knockdown of MSI2 expression did the opposite. Dysregulated KLF4/MSI2 signaling promotes PDAC progression and metastasis. PMID: 27449499
MSI1 and MSI2 bind and regulate the mRNA stability and translation of proteins operating in essential oncogenic signaling pathways..This review provides a current overview of Musashi as a cancer driver and novel therapeutic target. PMID: 28143872
High MSI2 expression is associated with esophageal squamous cell carcinoma. PMID: 29054489
High Musashi2 expression is associated with pancreatic cancer. PMID: 27092875
MSI2 regulates myeloid leukemia stem cells gene expression. PMID: 28436985
these findings provide evidence that DBC2 suppresses tumorigenesis in breast cancer by ubiquitinating MSI2. PMID: 27941885
Differential methylation of the MSI2 gene (chr17:55484635) in blood and islet cells is strongly related to hyperglycemia. PMID: 28542303
our findings identified a novel regulatory mechanism of MSI2 as an upstream regulator of ESR1 and revealed the clinical relevance of the RNA-binding protein MSI2 in breast cancer. PMID: 27593929
MSI2 and FLT3 are significantly co-regulated in human AML PMID: 28107692
MSI2 provides essential support for TGFbetaR1/SMAD3 signaling and contributes to invasive adenocarcinoma of the lung and may serve as a predictive biomarker of NSCLC aggressiveness PMID: 27274057
Overexpression of Msi2 significantly increased and silencing of Msi2 reduced the phosphorylation of JAK2 and its downstream effecter STAT3. PMID: 27322953
MSI2 overexpression induces paclitaxel resistance in ovarian cancer. PMID: 27600258
These results indicate that the human MSI2 gene might be a susceptibility gene for schizophrenia and encourage future research on the functional relationship between this gene and schizophrenia. PMID: 27059221
our study indicates that MSI-2 overexpression is associated with an unfavorable prognosis and may be a potential biomarker for liver metastasis in colorectal cancer patients. PMID: 26775684
The notion that regular and close monitoring of MSI2 mRNA levels in chronic myeloid leukemia patients might identify patients at risk of progressing to blast crisis was not supported by this study; an increase in MSI2 transcripts does not precede an increase in BCR-ABL1 mRNA levels. PMID: 27160312
Concomitant loss of function of both MSI1 is sufficient to abrogate the growth of human colorectal cancer cells, and Msi gene deletion inhibits tumorigenesis in several mouse models of intestinal cancer. PMID: 26673327
MSI2 directly attenuates aryl hydrocarbon receptor (AHR) signalling through post-transcriptional downregulation of canonical AHR pathway components in cord blood haematopoietic stem and progenitor cells PMID: 27121842
Up-regulated MSI2 is associated with more aggressive chronic myeloid leukemia. PMID: 25363400
This review highlights both the essential contribution of RBPs to posttranscriptional regulation and the importance of the Musashi family as master regulators of male gamete development--{REVIEW} PMID: 25851660
These results confirm the association of MSI2 expression with outcome in adult B-ALL and demonstrate the utility of MSI2 as a clinical prognostic biomarker. PMID: 25090928
Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. PMID: 25380226
the MSI2 RNA-binding protein has a role in transformation of intestinal epithelium PMID: 25774828
MSI2 controls efficient translation of the oncogenic leukemic stem cell self-renewal program. PMID: 25664853
Acute myeloid leukemia patients have a higher level of MSI2 gene expression. PMID: 25449073
MSI2 correlates with epithelial-mesenchymal transition and has the potential to be a new predictive biomarker of hepatocellular carcinoma prognosis and invasion. PMID: 24305552
Report a statistical difference between the MSI2 gene expression in different gastric tumor grades, of note between grade I and grade II. PMID: 24002004
The Msi2 plays an important role in leukemogenesis involving the MAPK signaling pathway, which indicates that Msi2 may be a novel target for leukemia treatment. PMID: 24076374
a large set of SOX2-associated proteins in DAOY medulloblastoma cells PMID: 23667531
Data suggest that high MSI2 expression could indicate poor prognosis and facilitate risk and treatment stratification in adult BCR-ABL-negative B-cells acute lymphoblastic leukemia (B-ALL). PMID: 23759245
MSI2 is associated with shorter overall survival in acute myeloid leukemia patients. PMID: 23233047
Down-modulation of MSI2 is associated with myelodysplastic syndromes and acute myeloid leukemia. PMID: 22784712
Overexpression of MSI2 resulted in a decrease in MSI1 expression. PMID: 22113197
High MSI2 is associated with acute myeloid leukemia. PMID: 21753187
MSI2 has a crucial role in both normal and malignant hematopoiesis. PMID: 20823875
Msi2 expression is not only highly upregulated during human chronic myeloid leukemia progression but is also an early indicator of poorer prognosis PMID: 20639863
The EVI1 gene locus was rearranged in all 13 myeloid malignancies patients and was associated with EVI1 overexpression. In 9 out of 13 patients, the 17q breakpoints clustered in a 250 kb region on band 17q22 encompassing the MSI2 gene PMID: 18815193

Hide All

Involvement in disease

Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).

Subcellular Location

Cytoplasm.

Protein Families

Musashi family

Tissue Specificity

Ubiquitous; detected at low levels.

Database Links

HGNC: 18585

OMIM: 607897

KEGG: hsa:124540

STRING: 9606.ENSP00000284073

UniGene: Hs.658922

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Code	CSB-RA099573A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate(30µg), U-251MG whole cell lysate(30µg), HT-29 whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: MSI2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 35 kDa Observed band size: 36 kDa Exposure time：2min40s Overlay Peak curve showing Hela cells stained with CSB-RA099573A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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MSI2 Recombinant Monoclonal Antibody

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Target Background