Product Details

Uniprot No.

Target Names

Alternative Names

Epididymis luminal protein 70 antibody; HEL70 antibody; Membrane organizing extension spike protein antibody; Membrane-organizing extension spike protein antibody; MOES_HUMAN antibody; Moesin antibody; Moesin/anaplastic lymphoma kinase fusion protein antibody; Msn antibody; MSN/ALK fusion antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human MSN

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Moesin belongs to the ERM (ezrin-radixin-moesin) protein family, serving as a critical linker between the actin cytoskeleton and the plasma membrane. This membrane-organizing protein plays essential roles in cell morphology, migration, and signal transduction, making it a key target for researchers investigating cytoskeletal dynamics, cancer metastasis, and immune cell function. The MSN/ALK fusion variant has also emerged as a significant focus in oncology research.

This recombinant monoclonal antibody (clone 8G8) offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human moesin and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across studies.

Validation data demonstrates reliable performance across multiple platforms. Western blot analysis detects a clean band at the predicted 68 kDa molecular weight across diverse human cell lines including MCF-7, U-251MG, PC-3, and K562, as well as mouse NIH/3T3 cells, confirming cross-species reactivity between human and mouse samples. Immunohistochemistry staining in paraffin-embedded human lymph node tissue shows specific detection using standard citrate buffer antigen retrieval protocols. Flow cytometry validation in HeLa cells demonstrates clear positive signal separation from isotype controls, supporting intracellular detection applications.

The antibody's versatility across ELISA, Western blot, immunohistochemistry, and flow cytometry provides flexibility for multi-platform experimental designs. Researchers studying cytoskeletal regulation, tumor cell invasion, or immune cell biology will find this antibody particularly valuable for characterizing moesin expression and localization in both fixed tissues and cultured cell systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs. Modulates phagolysosomal biogenesis in macrophages. Participates also in immunologic synapse formation.

Gene References into Functions

These results suggested that strong moesin expression by malignant cells may help to determine patients with oral squamous cell carcinoma and poor prognosis. PMID: 29310601
Up-regulation of moesin expression in glioblastoma cells resulted in more aggressive orthotopic glioblastoma growth in nude mice. PMID: 29848673
In lip squamous carcinoma, moesin expression was strong at the invasive tumor front and weak/negative in differentiated cells. There was no association between moesin expression and the clinicopathological variables, but high moesin was associated with lower 5- and 10-year overall and disease-free survival rates. Moesin may participate in oral carcinogenesis. PMID: 29715096
This review focuses on the role of moesin in microvascular permeability and angiogenesis, and on the involvement of Src and ROS in endothelial barrier disruption. PMID: 28129474
CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family. PMID: 27793041
The authors demonstrated that the expression of MSN in glioma specimens were negatively correlated with miR-200c expression, and MSN overexpression rescued the phenotype about cell proliferation and invasion induced by miR-200c. PMID: 28529643
The expression pattern and subcellular localization of ezrin and moesin correlate with clinicopathological variables such as patients' age, tumor grade and hormonal status. PMID: 28624994
Moesin and merlin regulate urokinase receptor-dependent endothelial cell migration, adhesion and angiogenesis PMID: 28473293
The results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site. PMID: 28889652
These results indicate that loss of miR-200c, as a consequence of p53 mutation, can upregulate Moesin oncogene and thus promote carcinogenesis in breast cancer PMID: 28933253
the administration of 10(-6) M retinoic acid (10-20 min) induces the activation of the migration-related proteins Moesin, FAK, and Paxillin in T-47D breast cancer cells. PMID: 27130522
Up-regulation of moesin expression in glioblastoma cells correlated with increases in cell proliferation, invasion and migration, suggesting moesin's role in glioblastoma progression. PMID: 28476784
this study identifies X-linked primary immunodeficiency associated with hemizygous mutations in the moesin gene PMID: 27405666
These results of this study may pave the way for exploiting moesin as a novel target for intervention in muscular dystrophy. PMID: 28082118
The present study showed over-expression of ezrin and moesin in colorectal carcinoma PMID: 27042764
These results indicate that the Thr 558 phosphorylation in moesin mediates endothelial angiogenesis. Advanced glycation end products promoted human umbilical vein endothelial cell angiogenesis by inducing moesin phosphorylation via RhoA/ROCK pathway. PMID: 26956714
Phospho-Ezrin/Radixin/Moesin (ERM) inhibit cell adhesion, and therefore, dephosphorylation of ERM proteins is essential for cell adhesion.Phospho-ERM induce formation and/or maintenance of spherical cell shape. PMID: 26555866
Moesin was required in HMGB1-induced F-actin rearrangement, hyperpermeability, and inflammatory responses. HMGB1 increased Thr558 phosphorylation of moesin. Moesin was elevated in sepsis. PMID: 25947626
results indicate Moesin may regulate cell motility through its interactions with MT1-MMP and E-cadherin/p120-catenin adhesion complex and cytoplasmic expression of Moesin correlates with nodal metastasis and poor prognosis of OSCCs PMID: 26194050
Intracellular sphingosine kinase 2-derived sphingosine-1-phosphate mediates epidermal growth factor-induced ezrin-radixin-moesin phosphorylation and cancer cell invasion. PMID: 26209696
Moesin might be involved in renal fibrosis and its effects could be related to interacting with E-Cadherin. PMID: 25406076
Ezrin, radixin and moesin are unlikely targets for autoantibodies in demyelinating neuropathies. PMID: 25286001
Ezrin and moesin expression is related to LSCC invasion and metastasis, and may be important molecular markers for predicting prognosis and therapeutic targets in LSCC patients PMID: 25299115
Data show that silencing ezrin-radixin-moesin (ERM) protein expression ablates deleted in colorectal carcinoma protein (DCC)-protein kinase A (PKA) interaction and specifically blocks netrin-induced PKA activity and phosphorylation. PMID: 25575591
both moesin-mediated inhibition and its localized deactivation by myosin phosphatase are essential for neutrophil polarization and effective neutrophil tracking of pathogens. PMID: 25601651
STIP1 and moesin may be novel and differential sero-diagnostic markers for psoriasis vulgaris and psoriatic arthritis PMID: 25010044
present findings suggest that moesin is characteristically overexpressed and activated in adenomyosis, and that moesin activation may be related to extension of adenomyosis in the myometrium. PMID: 24698421
Moesin was shown to be directly targeted by miR-200b; in breast cancer cell lines and patient samples, its expression is inversely correlated with miR-200 expression, and high levels of moesin were associated with poor relapse-free survival. PMID: 24037528
Moesin expression and CD44 expression might be useful markers of poor prognosis in gastric adenocarcinoma. PMID: 23889297
VIP regulates CFTR membrane expression and function in Calu-3 cells by increasing its interaction with NHERF1 and P-ERM in a VPAC1- and PKCepsilon-dependent manner. PMID: 24788249
Talin has a role in regulating moesin-NHE-1 recruitment to invadopodia in breast cancer PMID: 24891603
Phosphorylation of moesin at Thr558 by JNK is important for podosome rosette formation. PMID: 24127566
Testosterone induces actin cytoskeleton remodeling by regulating ROCK-2/moesin expression and activation. PMID: 24065547
The aim of this study was to examine the expression of ERM (ezrin, moesin) and Rho (RhoA, RhoB and Cdc42) proteins in breast cancer (BC) patients. PMID: 23420497
important role of ezrin-moesin-radixin proteins during HCV infection at the postentry level and highlight possible novel targets for HCV treatment. PMID: 23703860
Molecular basis for the moesin/l-selectin/CaM ternary complex; an important role for phospholipids in modulating l-selectin function and shedding. PMID: 23796515
ERM proteins play important differential roles in the thrombin-induced modulation of EC permeability, with moesin promoting barrier dysfunction and radixin opposing it. PMID: 23729486
These results suggest moesin protein involved in the genesis and progression of astrocytomas PMID: 23315217
Data suggest that P-glycoprotein associate with the F-actin cytoskeleton through ezrin/radixin/moesin (ERM) in CCR9/CCL25 induced multidrug resistance of acute T-lymphocytic leukemia (T-ALL) cells. PMID: 23326330
Control of adipogenesis by ezrin, radixin and moesin-dependent biomechanics remodeling. PMID: 23116763
Moesin is a glioma progression marker that induces proliferation and Wnt/beta-catenin pathway activation via interaction with CD44. PMID: 23221384
Ezrin/radixin/moesin are required for the purinergic P2X7 receptor (P2X7R)-dependent processing of the amyloid precursor protein. PMID: 22891241
These results offer biological evidence of moesin as an EMT marker, support the association between moesin, Snail and EMT and suggest a role for moesin in breast cancer prognostication. PMID: 22439598
role of CCM3 and ezrin/radixin/moesin family of proteins in cell's response to oxidative stress PMID: 22291017
Ano1, ezrin, and moesin/radixin colocalize apically in salivary gland epithelial cells, and overexpression of moesin and Ano1 in HEK cells alters the subcellular localization of both proteins PMID: 22685202
Both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. PMID: 22076435
increased moesin expression promotes EMT by regulating adhesion and contractile elements for changes in actin filament organization. PMID: 22031288
Activation of moesin, a protein that links actin cytoskeleton to the plasma membrane, occurs by phosphatidylinositol 4,5-bisphosphate (PIP2) binding sequentially to two sites and releasing an autoinhibitory linker. PMID: 22433855
Results confirm the involvement of RhoA/ROCK pathway and subsequent moesin Thr558 phosphorylation in advanced glycation endproduct-mediated endothelial dysfunction. PMID: 22251897
Knockdown of moesin did not influence the expression or function of P-gp PMID: 21837648

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Involvement in disease

Immunodeficiency 50 (IMD50)

Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, microvillus.

Tissue Specificity

In all tissues and cultured cells studied.

Database Links

HGNC: 7373

OMIM: 300988

KEGG: hsa:4478

STRING: 9606.ENSP00000353408

UniGene: Hs.713679

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Code	CSB-RA257452A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), PC-3 whole cell lysate(30µg), K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: Moesin antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 3min IHC image of CSB-RA257452A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA257452A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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MSN Recombinant Monoclonal Antibody

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Target Background