Mono-methyl-Histone H3.1（K18）Antibody

^Datasheet

Code	CSB-RA010418A18me1HU
Size	US$350
Image	Western Blot Positive WB detected in：MCF-7 whole cell lysate，Hela whole cell lysate，HepG2 whole cell lysate，SH-SY5Y whole cell lysate,Rat brain tissue All lanes：Mono-methyl-Histone H3.1（K18）antibody at 0.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa Immunocytochemistry analysis of CSB-RA010418A18me1HU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-RA010418A18me1HU at 1:43,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Product Details
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Target Data

Product Details

Uniprot No.

P68431

Target Names

HIST1H3A

Alternative Names

Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, AND, HIST1H3J, H3FJ

Species Reactivity

Human

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, ICC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
ICC	1:50-1:500
IF	1:30-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease	Glioma (GLM)
Subcellular Location	Nucleus, Chromosome
Protein Families	Histone H3 family
Database Links	HGNC: 4766 OMIM: 137800 KEGG: hsa:8350 STRING: 9606.ENSP00000444823 UniGene: Hs.132854

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