Code | CSB-RA010418A17me1HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
The production of the mono-methyl-Histone H3.1 (R17) recombinant monoclonal antibody is a stepwise procedure that begins with the cloning of genes encoding the HIST1H3A antibody, which includes both the heavy and light chains. These cloned genes are then inserted into expression vectors designed for optimal performance. Following this, the modified expression vectors are introduced into host cells through transfection, where the host cells undertake the task of producing and secreting the antibody. The purified antibody is obtained through affinity chromatography to ensure its purity and functionality. To guarantee its effectiveness, the antibody undergoes a series of rigorous tests across diverse applications, such as ELISA, WB, ICC, and IF, tailored for the precise detection of the human HIST1H3A protein mono-methylated at R17.
Mono-methylation of Histone H3.1 at arginine 17 (R17) is involved in transcriptional regulation, chromatin structure, DNA repair, cellular identity, and epigenetic signaling, and has implications in various diseases.
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