Code | CSB-RA010418A02me1HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
The mono-methyl-histone H3.1 (R2) recombinant monoclonal antibody production involves cloning genes encoding for the HIST1H3A antibody and expressing them in mammalian cell expression systems. The genes encoding the heavy and light chains of the HIST1H3A antibody are cloned into expression vectors, which are then introduced into host cells via transfection. The host cells then produce and secrete the antibodies, which can be purified through affinity chromatography. The resulting antibody has been tested for functionality in ELISA, WB, ICC, and IF applications for the detection of human HIST1H3A protein mono-methylated at R2.
Mono-methylation of HIST1H3A at arginine 2 (R2) is an epigenetic modification that can influence gene expression and chromatin structure, particularly promoting gene activation and accessibility. Its dynamic regulation is crucial for maintaining cellular identity and responding to environmental cues, with potential implications in various diseases.
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